Abstract:
:The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE N1 replacing the counter part of Escherichia coli GBE) mutants were constructed. The truncation mutants synthesized branched products with a greatly reduced proportion of short chains. The swapping mutant exhibited a branching pattern of the short chain region similar to that of VvGBE. We conclude that the N1-domain of VvGBE has a crucial role in the determination of the branching pattern of glycogen.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jo HJ,Park S,Jeong HG,Kim JW,Park JTdoi
10.1016/j.febslet.2015.03.011subject
Has Abstractpub_date
2015-04-28 00:00:00pages
1089-94issue
10eissn
0014-5793issn
1873-3468pii
S0014-5793(15)00187-8journal_volume
589pub_type
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