Abstract:
:The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. The endogenous gene of yukD in B. subtilis was disrupted without an obvious phenotypic effect and an inducible copy encoding a C-Myc and His-tagged version of the protein was introduced at the ectopic locus amyE. Conjugation assays performed both in vitro and in vivo indicate that YukD lacks the capacity for covalent bond formation with other proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
van den Ent F,Löwe Jdoi
10.1016/j.febslet.2005.06.002keywords:
subject
Has Abstractpub_date
2005-07-04 00:00:00pages
3837-41issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00711-8journal_volume
579pub_type
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