Abstract:
:Copper(II) was substituted for manganese(III) in Bacillus stearothermophilus Mn-superoxide dismutase. The (EPR) spectrum of the Cu(II) is distinctly rhombic, overlapping that of Cu(II) replacing zinc in copper/zinc superoxide dismutase. The copper-cyanide complex of the bacterial enzyme gives an EPR spectrum nearly identical to the cyanide-copper complex of the Cu/Zn enzyme, indicating three nitrogens as metal ligands. These results support the suggestion that metal coordination in B. stearothermophilus Mn-dismutase is a tetrahedral arrangement of three imidazoles and a carboxylate group and indicate copper as a good spectroscopic probe for studying the active site of Mn- and Fe-superoxide dismutases.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bannister JV,Desideri A,Rotilio Gdoi
10.1016/0014-5793(85)80881-4subject
Has Abstractpub_date
1985-08-19 00:00:00pages
91-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)80881-4journal_volume
188pub_type
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