Abstract:
:A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that the amino acid sequence of Na(+)-ATPase alpha-subunit is highly homologous to the corresponding sequences of large (alpha) subunits of vacuolar (archaebacterial) type H(+)-ATPases, supporting our proposal [Kakinuma, Y. and Igarashi, K. (1990) FEBS Lett. 271, 97-101] that the Na(+)-ATPase of this organism belongs to the vacuolar-type ATPase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kakinuma Y,Igarashi K,Konishi K,Yamato Idoi
10.1016/0014-5793(91)80835-qsubject
Has Abstractpub_date
1991-11-04 00:00:00pages
64-8issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80835-Qjournal_volume
292pub_type
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