Abstract:
:By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumping clones, J21-045 traverses 210 kbp and links NL2-252 to NL26 (D3S1632), a NotI linking clone containing highly polymorphic sequences. The TFRC gene was mapped to 3q29, close to the telomeric marker D3S2344, by linkage analysis, a panel of hybrid cell lines, GeneBridge 4 panel and FISH. Clone NLM-007 (D3S4302) was found to contain ras-homologous gene RAB7. By FISH and a panel of hybrid cell lines this gene was mapped to 3q21. This region is of particular interest due to frequent rearrangements in different types of leukemia. Clone L2-081 (D3S4283) containing new member of ubiquitin-specific proteases (HAUSP gene) was localized in 3p21 inspiring further investigation of involvement of this gene in development of lung and renal carcinomas.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kashuba VI,Gizatullin RZ,Protopopov AI,Allikmets R,Korolev S,Li J,Boldog F,Tory K,Zabarovska V,Marcsek Z,Sumegi J,Klein G,Zabarovsky ER,Kisselev Ldoi
10.1016/s0014-5793(97)01449-xsubject
Has Abstractpub_date
1997-12-15 00:00:00pages
181-5issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01449-Xjournal_volume
419pub_type
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