Abstract:
:The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), using electron spin resonance (ESR) and ESR spin trapping methodology. The spin trap used was 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). It is found that the oxidation of these thiols by vanadium(V) generates the corresponding thiyl radicals and vanadium- (IV) complexes. The results suggest that free radical reactions play a significant role in the depletion of cellular thiols by vanadium(V) and hence in vanadium(V) toxicity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Shi XL,Sun XY,Dalal NSdoi
10.1016/0014-5793(90)80402-5subject
Has Abstractpub_date
1990-10-01 00:00:00pages
185-8issue
1-2eissn
0014-5793issn
1873-3468journal_volume
271pub_type
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