Abstract:
:A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have an important influence on glycosylation. The expression system was used to screen mycobacterial sequences selected using a neural network (NetOglyc) trained on eukaryotic O-glycoproteins. Evidence of glycosylation was obtained for eight of 11 proteins tested. The results suggest that sites involved in O-glycosylation of mycobacterial and eukaryotic proteins share similar structural features.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Herrmann JL,Delahay R,Gallagher A,Robertson B,Young Ddoi
10.1016/s0014-5793(00)01553-2keywords:
subject
Has Abstractpub_date
2000-05-19 00:00:00pages
358-62issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01553-2journal_volume
473pub_type
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