Autocatalytic processes in cooperative mechanisms of prion diseases.

abstract：:Serine/threonine phosphatases such as PP1, PP2A, and PP2B are well known to regulate the transition phase of the cell cycle. However, the function of PP2Cgamma in cell cycle progression is still unclear. In the present study, we report the characterization of PP2Cgamma in mammalian cells during the cell cycle. After r...

journal_title：FEBS letters

authors： Suh EJ,Kim TY,Kim SH

更新日期：2006-11-13 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...

journal_title：FEBS letters

authors： Pellicone C,Nullans G,Virmaux N

更新日期：1985-02-11 00:00:00

abstract：:Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...

journal_title：FEBS letters

authors： Sugihara K,Shibata TK,Takata K,Kimura T,Kanayama N,Williams R,Hatakeyama S,Akama TO,Kuo CW,Khoo KH,Fukuda MN

更新日期：2013-10-01 00:00:00

abstract：:Water-soluble amyloid beta-peptides (sA beta), ending at residue 42, precede amyloid plaques in Down's syndrome (DS). Here we report that sA beta consists of the full-length A beta(1-42) and peptides truncated and modified by cyclization of the N-terminal glutamates, A beta[3(pE)-42] and A beta[11(pE)-42]. The A beta[...

journal_title：FEBS letters

authors： Russo C,Saido TC,DeBusk LM,Tabaton M,Gambetti P,Teller JK

更新日期：1997-06-16 00:00:00

abstract：:Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...

journal_title：FEBS letters

authors： Kryukov VM,Ksenzenko VN,Kaliman AV,Bayev AA

更新日期：1983-07-11 00:00:00

abstract：:The neopterin derivatives, neopterin and 7,8-dihydroneopterin, modulate the cellular oxidant-antioxidant balance as well as the expression of the inducible nitric oxide synthase (iNOS) gene. Since apoptosis can be induced by reactive oxygen intermediates and nitric oxide (NO) we investigated whether these neopterin de...

journal_title：FEBS letters

authors： Schobersberger W,Hoffmann G,Hobisch-Hagen P,Bock G,Volkl H,Baier-Bitterlich G,Wirleitner B,Wachter H,Fuchs D

更新日期：1996-11-18 00:00:00

abstract：:It was recently shown that there is a predominance of phase 1 introns near the cleavage site of signal peptides encoded by human genes. It was suggested that this biased distribution was due to intron insertion at AGmid R:G proto-splice sites. However, we found that there is no disproportional excess of AGmid R:G that...

journal_title：FEBS letters

authors： Vibranovski MD,Sakabe NJ,de Souza SJ

更新日期：2006-03-06 00:00:00

abstract：:A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secr...

journal_title：FEBS letters

authors： Vogel LK,Suske G,Beato M,Norén O,Sjöström H

更新日期：1993-09-20 00:00:00

abstract：:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

journal_title：FEBS letters

authors： Shuvaev VV,Siest G

更新日期：1996-03-25 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:A cDNA encoding a novel plant type III polyketide synthase (PKS) was cloned from rhubarb (Rheum palmatum). A recombinant enzyme expressed in Escherichia coli accepted acetyl-CoA as a starter, carried out six successive condensations with malonyl-CoA and subsequent cyclization to yield an aromatic heptaketide, aloesone...

journal_title：FEBS letters

authors： Abe I,Utsumi Y,Oguro S,Noguchi H

更新日期：2004-03-26 00:00:00

abstract：:Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as sugg...

journal_title：FEBS letters

authors： Knorre DG,Buneva VN,Baram GI,Godovikova TS,Zarytova VF

更新日期：1986-01-01 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presenc...

journal_title：FEBS letters

authors： Leboulle G,Müller U

更新日期：2004-10-08 00:00:00

abstract：:The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...

journal_title：FEBS letters

authors： Eichenberger W,Grob EC

更新日期：1970-12-01 00:00:00

abstract：:The Escherichia coli twin-arginine translocation (Tat) system serves to export fully folded protein substrates across the bacterial cytoplasmic membrane. Respiratory [NiFe] hydrogenases are synthesised as precursors with twin-arginine signal peptides and transported as large, cofactor-containing, multi-subunit complex...

journal_title：FEBS letters

authors： Dubini A,Sargent F

更新日期：2003-08-14 00:00:00

abstract：:Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak, a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having beta (beta I and beta II)-sequence and type III having alpha-sequence, respe...

journal_title：FEBS letters

authors： Hashimoto K,Kishimoto A,Aihara H,Yasuda I,Mikawa K,Nishizuka Y

更新日期：1990-04-09 00:00:00

abstract：:Pleckstrin homology (PH) domains are protein modules found in proteins involved in many cellular processes. The majority of PH domain-containing proteins require membrane association for their function. It has been shown that most PH domains interact directly with the cell membrane by binding to phosphoinositides with...

journal_title：FEBS letters

authors： Maffucci T,Falasca M

更新日期：2001-10-12 00:00:00

abstract：:The interaction between two codon-anticodon duplexes of the ribosomal A- and P-site-bound tRNAs is the key feature of the proposed model. This interaction prohibits non-canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the...

journal_title：FEBS letters

authors： Lim VI,Venclovas C

更新日期：1992-11-23 00:00:00

abstract：:Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...

journal_title：FEBS letters

authors： Olins GM,Mehta PP,Blehm DJ,Patton DR,Zupec ME,Whipple DE,Tjoeng FS,Adams SP,Olins PO,Gierse JK

更新日期：1987-11-30 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

journal_title：FEBS letters

authors： Hesketh JE

更新日期：1984-04-24 00:00:00

abstract：:vp165 (or gp160) is an aminopeptidase that has been identified as one of the major proteins of the GLUT4-containing vesicles. In the present study we have determined the degree of co-localization between vp165 and GLUT4 in rat adipose cells and used perturbation by wortmannin to assess the exocytic and endocytic steps...

journal_title：FEBS letters

authors： Malide D,St-Denis JF,Keller SR,Cushman SW

更新日期：1997-06-16 00:00:00

abstract：:We have characterized the cell surface of zebrafish stratified epithelium using a combined approach of light and atomic force microscopy under conditions which simulate wound healing. Microridges rise on average 100 nm above the surface of living epithelial cells, which correlate to bundles of cytochalasin B-insensiti...

journal_title：FEBS letters

authors： Sharma A,Anderson KI,Müller DJ

更新日期：2005-03-28 00:00:00

abstract：:Fas ligand (FasL) binds Fas (CD95) to induce apoptosis or activate other signaling pathways. In addition, FasL transduces bidirectional or 'reverse signals'. The intracellular domain of FasL contains consensus sequences for phosphorylation and an extended proline rich region, which regulate its surface expression thro...

journal_title：FEBS letters

authors： Thornhill PB,Cohn JB,Drury G,Stanford WL,Bernstein A,Desbarats J

更新日期：2007-09-18 00:00:00

abstract：:The exposure of cells for 60 min to a serum free medium at ice temperature followed by a return to normal culture conditions (30 min at 37 degrees C) caused a dramatic decrease in NAD+ levels. This decrease in NAD+ was prevented by 3-aminobenzamide. Alkaline elution analysis of DNA from cultures that were sisters to t...

journal_title：FEBS letters

authors： Cantoni O,Sestili P,Cattabeni F,Stocchi V

更新日期：1986-08-18 00:00:00

abstract：:We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovir...

journal_title：FEBS letters

authors： Toyama R,Okayama H

更新日期：1990-07-30 00:00:00

abstract：:The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzym...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV,Brown GC

更新日期：1994-07-25 00:00:00

abstract：:Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...

journal_title：FEBS letters

authors： Demeshkina N,Jenner L,Westhof E,Yusupov M,Yusupova G

更新日期：2013-06-27 00:00:00