Abstract:
:A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' terminus of the poly(A) tail. Among these proteins, we focused on La motif-related protein 1 (LARP1) and found that LARP1 specifically recognizes the 3' termini of normal poly(A) tails. We also reveal that LARP1 stabilizes multiple mRNAs carrying 5' terminal oligopyrimidine tract (5'TOP). Our findings suggest that LARP1 may be involved in the post-transcriptional regulation of gene expression, at least in several 5'TOP mRNAs, through the binding to 3' terminus of the poly(A) tail.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Aoki K,Adachi S,Homoto M,Kusano H,Koike K,Natsume Tdoi
10.1016/j.febslet.2013.05.035subject
Has Abstractpub_date
2013-07-11 00:00:00pages
2173-8issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(13)00389-Xjournal_volume
587pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80549-v
更新日期:1992-02-10 00:00:00
abstract::A stable complex of the chaperonins, cpn60 and cpn10 (Escherichia coli GroEL and GroES homologues), from the extremely thermophilic bacterium Thermus thermophilus has been isolated and crystallized. The crystals have dimensions up to 30 x 200 x 200 microns. Ultra-thin sections of the crystals estimated by electron mic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81357-r
更新日期:1992-10-12 00:00:00
abstract::The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81229-8
更新日期:1988-08-01 00:00:00
abstract::Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00997-7
更新日期:1999-08-20 00:00:00
abstract::Subclinical inflammation is a candidate etiological factor in the pathogenesis of metabolic syndrome and in the progression of atherosclerosis. A central role for activated macrophages has been elucidated recently as important regulators of the inflammatory process in atherosclerosis. Macrophage differentiation and fu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.11.020
更新日期:2008-01-09 00:00:00
abstract::Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.022
更新日期:2006-02-06 00:00:00
abstract::The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80437-x
更新日期:1988-06-20 00:00:00
abstract::Vacuolar tandem-pore channels could not be analysed in Xenopus oocytes so far, due to misguided translocation. Owing to the conservation of their pore regions, we were able to prepare functional pore-chimeras between the plasma membrane localised TPK4 and vacuolar TPKs. Thereby, we found evidence that TPK2, TPK3 and T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.038
更新日期:2010-06-03 00:00:00
abstract::A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81239-k
更新日期:1990-09-17 00:00:00
abstract::We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.028
更新日期:2009-01-05 00:00:00
abstract::The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.01.051
更新日期:2010-03-05 00:00:00
abstract::The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80554-t
更新日期:1992-02-10 00:00:00
abstract::Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance sp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.016
更新日期:2008-12-24 00:00:00
abstract::A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80774-b
更新日期:1992-07-28 00:00:00
abstract::Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.038
更新日期:2010-03-19 00:00:00
abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.034
更新日期:2007-05-15 00:00:00
abstract::Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.036
更新日期:2015-12-21 00:00:00
abstract::We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80576-o
更新日期:1991-05-20 00:00:00
abstract::Auxin increases phospholipase A(2) activity within 2min (Paul, R., Holk, A. and Scherer, G.F.E. (1998) Fatty acids and lysophospholipids as potential second messengers in auxin action. Rapid activation of phospholipase A(2) activity by auxin in suspension-cultured parsley and soybean cells. Plant J. 16, 601-611) and t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.059
更新日期:2007-09-04 00:00:00
abstract::Coeliac disease is a chronic enteropathy caused by the ingestion of wheat gliadin and other cereal prolamines derived from rye and barley. In the present work, we investigated the mechanisms underlying altered barrier function properties exerted by gliadin-derived peptides in human Caco-2 intestinal epithelial cells. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.066
更新日期:2005-08-29 00:00:00
abstract::A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.044
更新日期:2009-04-02 00:00:00
abstract::Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.003
更新日期:2007-06-12 00:00:00
abstract::Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03249-0
更新日期:2002-09-11 00:00:00
abstract::Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K(+) to cause a marked enhancement of ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01115-1
更新日期:1999-10-01 00:00:00
abstract::Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.012
更新日期:2006-05-15 00:00:00
abstract::Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12794
更新日期:2017-09-01 00:00:00
abstract::A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80390-1
更新日期:1983-02-21 00:00:00
abstract::We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00876-0
更新日期:1994-09-05 00:00:00
abstract::Monocyte chemoattractant protein-1 (MCP-1) is a member of the beta chemokine family which acts through specific seven transmembrane receptors to recruit monocytes, basophils, and T lymphocytes to sites of inflammation. To identify regions of the human MCP-1 protein which are important for its biological activity, we h...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00637-1
更新日期:1998-07-03 00:00:00
abstract::In addition to their g = 1.94 EPR signal, nitrogenase Fe-proteins from Azotobacter vinelandii, Azotobacter chroococcum and Klebsiella pneumoniae exhibit a weak EPR signal with g approximately equal to 5. Temperature dependence of the signal was consistent with an S = 3/2 system with negative zero-field splitting, D = ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81033-4
更新日期:1985-09-23 00:00:00