Abstract:
:Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is detectable by Northern analysis. The other two genes are transcribed at basal levels detectable only by reverse transcription PCR. One of the other two genes (rti-2) encodes a polypeptide with a glutamic residue in the P1 position, characteristic of glutamyl proteinase inhibitors. The recombinant RTI-2 protein strongly inhibits (Ki=44 nM) a glutamyl proteinase from Streptomyces griseus.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
De Leo F,Volpicella M,Sciancalepore M,Gallerani R,Ceci LRdoi
10.1016/j.febslet.2006.01.022keywords:
subject
Has Abstractpub_date
2006-02-06 00:00:00pages
948-54issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00062-7journal_volume
580pub_type
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