Abstract:
:The 36 kDa fragment of actin molecule obtained with the protease from E. coli A2 strain [(1988) FEBS Lett. 228, 172] was shown to begin with Val-43 and retain the COOH-terminal amino acid residues of the parent molecule. The E. coli protease split actin preserves the NH2-terminal part of the polypeptide chain as well as the native conformation of actin molecule. However, the E. coli protease split actin failed to polymerize in 0.1 M KCl, suggesting that integrity of actin molecule between Gly-42 and Val-43 is crucial for actin polymerization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Khaitlina SYu,Collins JH,Kuznetsova IM,Pershina VP,Synakevich IG,Turoverov KK,Usmanova AMdoi
10.1016/0014-5793(91)80247-zsubject
Has Abstractpub_date
1991-02-11 00:00:00pages
49-51issue
1eissn
0014-5793issn
1873-3468journal_volume
279pub_type
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