Abstract:
:We report a nonenzymatic reaction which results in the radiolabeling of proteins by 32P-containing nucleoside triphosphates. The labeling reaction does not require any cofactors, but is greatly enhanced by the presence of alcohols. Even under optimal conditions, less than 1% of the protein molecules undergo modification. This nonspecific labeling represents a serious artifact which may become significant in systems involving low levels of specific labeling, such as photoaffinity labeling. Since the reaction is not limited to specific proteins, this may, however, provide a simple and rapid procedure for the preparation of labeled proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schmidt MC,Hanna MMdoi
10.1016/0014-5793(86)80106-5subject
Has Abstractpub_date
1986-01-06 00:00:00pages
305-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80106-5journal_volume
194pub_type
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