Abstract:
:Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demonstrates that a single missense mutation at the level of the gene encoding the peripheral 49-kDa/NUOD subunit of Complex I is definitely associated with this resistance. Based on this original observation, we propose a model locating the binding site for piericidin (and quinone) at the interface between the hydrophilic and hydrophobic domains of Complex I.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Darrouzet E,Issartel JP,Lunardi J,Dupuis Adoi
10.1016/s0014-5793(98)00719-4subject
Has Abstractpub_date
1998-07-10 00:00:00pages
34-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00719-4journal_volume
431pub_type
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