Prion protein is a component of the multimolecular signaling complex involved in T cell activation.


:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP(C) and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP(C) interacts with ZAP-70, suggesting that PrP(C) is a component of the multimolecular signaling complex within microdomains involved in T cell activation.




FEBS letters


Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M





Has Abstract


2004-02-27 00:00:00














  • In vivo glycosylation suppresses the aggregation of amyloidogenic hen egg white lysozymes expressed in yeast.

    abstract::The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Song Y,Azakami H,Hamasu M,Kato A

    更新日期:2001-02-23 00:00:00

  • Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.

    abstract::Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indica...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Despons L,Walter P,Senger B,Ebel JP,Fasiolo F

    更新日期:1991-09-09 00:00:00

  • Protein kinase C activity in rat skeletal muscle. Apparent relation to body weight and muscle growth.

    abstract::Protein kinase C (PKC) may be involved in growth regulation. In the present study the relationship between body weight, and thereby age, and the activity of PKC in muscle as well as in rapidly growing overloaded muscle were investigated. PKC activity in music was linearly inversely correlated to rat weight in both sol...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Richter EA,Nielsen NB

    更新日期:1991-09-02 00:00:00

  • The association of pp125FAK, pp60Src, CDC42Hs and Rap1B with the cytoskeleton of aggregated platelets is a reversible process regulated by calcium.

    abstract::The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dash D,Aepfelbacher M,Siess W

    更新日期:1995-04-24 00:00:00

  • Treatment with LXR agonists after focal cerebral ischemia prevents brain damage.

    abstract::Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sironi L,Mitro N,Cimino M,Gelosa P,Guerrini U,Tremoli E,Saez E

    更新日期:2008-10-15 00:00:00

  • Modulation of the alpha 1A Ca2+ channel by beta subunits at physiological Ca2+ concentration.

    abstract::The class A Ca2+ channel alpha 1 subunit (alpha 1A) was expressed in Xenopus oocytes alone or in combination with the beta 1b, beta 2a, beta 3, or beta 4 subunit. Analysis of voltage-dependent activation and inactivation in the presence of 1.8 mM external Ca2+ showed an hyperpolarising shift of both relations when com...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cens T,Mangoni ME,Nargeot J,Charnet P

    更新日期:1996-08-12 00:00:00

  • Expression of synaptotagmin and syntaxin associated with N-type calcium channels in small cell lung cancer.

    abstract::The presence of synaptic proteins involved in excitation/secretion coupling was examined in ten small cell lung cancer lines. N-Type calcium channels (omega-conotoxin receptors), synaptotagmin (p65) and syntaxin (HPC-1) were detected in eight. Co-immunoprecipitation experiments indicated that syntaxin can form a compl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: David P,el Far O,Martin-Mouto N,Poupon MF,Takahashi M,Seagar MJ

    更新日期:1993-07-12 00:00:00

  • Identification of a wheat germ agglutinin-sensitive ATPase in yeast nuclei.

    abstract::We have found that wheat germ agglutinin (WGA), a lectin that specifically binds to N-acetylglucosamine residues inhibits the in vitro transport of plasmid DNA, pJDB219, into yeast nuclei. Histochemical staining of the isolated nuclei with biotinylated WGA and streptavidin-biotinylated peroxidase complex revealed the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuchiya E,Hiraga K,Fukui S,Miyakawa T

    更新日期:1989-07-03 00:00:00

  • Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence.

    abstract::A novel gene for advanced red-shifted protein with an emission maximum at 593 nm was cloned from Discosoma coral. The protein, named dsFP593, is highly homologous to the recently described GFP-like protein drFP583 with an emission maximum at 583 nm. Using the remarkable similarity of the drFP583 and dsFP593 genes, we ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fradkov AF,Chen Y,Ding L,Barsova EV,Matz MV,Lukyanov SA

    更新日期:2000-08-18 00:00:00

  • Synergistic activation of insect cAMP-dependent protein kinase A (type II) by cyclicAMP and cyclicGMP.

    abstract::The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presenc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Leboulle G,Müller U

    更新日期:2004-10-08 00:00:00

  • Uteroglobin, an apically secreted protein of the uterine epithelium, is secreted non-polarized form MDCK cells and mainly basolaterally from Caco-2 cells.

    abstract::A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vogel LK,Suske G,Beato M,Norén O,Sjöström H

    更新日期:1993-09-20 00:00:00

  • Thrombin-induced inhibition of myoblast differentiation is mediated by Gbetagamma.

    abstract::Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagao M,Kaziro Y,Itoh H

    更新日期:2000-04-28 00:00:00

  • Stable insertion of Alzheimer Abeta peptide into the ER membrane strongly correlates with its length.

    abstract::Alzheimer's disease is characterized by the deposition of amyloid beta-peptide (Abeta) plaques in the brain. Full-length amyloid-beta precursor protein (APP) is processed by alpha- and beta-secretases to yield soluble APP derivatives and membrane-bound C-terminal fragments, which are further processed by gamma-secreta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lundin C,Johansson S,Johnson AE,Näslund J,von Heijne G,Nilsson I

    更新日期:2007-08-07 00:00:00

  • NAD(P)H- and superoxide-dependent nitric oxide degradation by rat liver mitochondria.

    abstract::Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oliveira HC,Saviani EE,Salgado I

    更新日期:2009-07-07 00:00:00

  • Ultraweak photoemission from dark-adapted leaves and isolated chloroplasts.

    abstract::Dark-adapted isolated spinach chloroplasts and leaves, unlike sub-chloroplast fractions, are capable of emitting ultraweak light spontaneously (50-125 counts/s per cm2). The emission of leaves is due to two processes with activation energies of 97 and 25 kJ/mol while in isolated chloroplasts, it is the result of a sin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hideg E,Kobayashi M,Inaba H

    更新日期:1990-11-26 00:00:00

  • The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site.

    abstract::Three proteins of the mitochondrial inner membrane are known that are essential for the viability of yeast and seem to be involved in import of preproteins; the integral membrane proteins MIM17 and MIM23 and the peripheral membrane protein MIM44, MIM17 and MIM23 are homologous to each other in their hydrophobic domain...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kübrich M,Keil P,Rassow J,Dekker PJ,Blom J,Meijer M,Pfanner N

    更新日期:1994-08-01 00:00:00

  • Mapping of binding sites for Mu repressor and ner product within the left-end EcoRI. C fragment of the Mu genome.

    abstract::Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Barlach S,Schumann W

    更新日期:1983-06-27 00:00:00

  • Differential regulation of the STARD1 subfamily of START lipid trafficking proteins in human macrophages.

    abstract::The STARD1 subfamily of 'START' lipid trafficking proteins can reduce macrophage lipid content and inflammatory status (STARD1; StAR), and traffic cholesterol from endosomes (STARD3/MLN64). During macrophage differentiation, STARD1 mRNA and protein increase with sterol content, while the reverse is true for STARD3. St...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borthwick F,Taylor JM,Bartholomew C,Graham A

    更新日期:2009-04-02 00:00:00

  • Beta-glucosidase activity towards a bile acid glucoside in human liver.

    abstract::Human liver contains hydrolytic activity toward 3 beta-glucosido-chenodeoxycholic acid. This beta-glucosidase activity, localized predominantly in the microsomal fraction, was optimally active in the presence of divalent metal ions close to pH 5.0 and was inhibited by EDTA. Kinetic parameters and other catalytic prope...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matern H,Gartzen R,Matern S

    更新日期:1992-12-14 00:00:00

  • Kinetics of inhibition of sperm beta-acrosin activity by suramin.

    abstract::Sperm beta-acrosin activity is inhibited by suramin, a polysulfonated naphthylurea compound with therapeutic potential as a combined antifertility agent and microbicide. A kinetic analysis of enzyme inhibition suggests that three and four molecules of suramin bind to one molecule of ram and boar beta-acrosins respecti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hermans JM,Haines DS,James PS,Jones R

    更新日期:2003-06-05 00:00:00

  • Induction and peroxisomal appearance of gulonolactone oxidase upon clofibrate treatment in mouse liver.

    abstract::Various antihyperlipemic peroxisome proliferators are known to be carcinogenic in rodents but not in human, other primates and guinea pig, which species lost their ability to synthesize ascorbate due to mutations in the gulonolactone oxidase gene. Ascorbate synthesis is accompanied by H2O2 production, consequently its...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Braun L,Mile V,Schaff Z,Csala M,Kardon T,Mandl J,Bánhegyi G

    更新日期:1999-09-24 00:00:00

  • How the protonation state of a phosphorylated amino acid governs molecular recognition: insights from classical molecular dynamics simulations.

    abstract::Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kawade R,Kuroda D,Tsumoto K

    更新日期:2020-03-01 00:00:00

  • On the substrate specificity of nitric oxide synthase.

    abstract::Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hecker M,Walsh DT,Vane JR

    更新日期:1991-12-09 00:00:00

  • Purification and characterization of ubiquitin from mammalian testis.

    abstract::Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved meth...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Loir M,Caraty A,Lanneau M,Menezo Y,Muh JP,Sautiere P

    更新日期:1984-04-24 00:00:00

  • Codon reading properties of an unmodified transfer RNA.

    abstract::We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Claesson C,Samuelsson T,Lustig F,Borén T

    更新日期:1990-10-29 00:00:00

  • Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique.

    abstract::Dynein is a minus end-directed microtubule motor that serves multiple cellular functions. We have performed a fine mapping of the 8 kDa dynein light chain (LC8) binding sites throughout the development of a library of consecutive synthetic dodecapeptides covering the amino acid sequences of the various proteins known ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rodríguez-Crespo I,Yélamos B,Roncal F,Albar JP,Ortiz de Montellano PR,Gavilanes F

    更新日期:2001-08-17 00:00:00

  • Activation of snake venom metalloproteinases by a cysteine switch-like mechanism.

    abstract::The cDNAs of several snake venom zinc endopeptidases code for a putative propeptide, which includes the conserved cysteine-containing sequence PKMCGVT. It has been suggested that binding of the cysteine thiol function to the active-site zinc, resulting in inactivation of the catalytic domain, occurs in a mode similar ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Grams F,Huber R,Kress LF,Moroder L,Bode W

    更新日期:1993-11-29 00:00:00

  • 5-Azacytidine increases the synthesis of embryonic hemoglobin (E2) in murine erythroleukemic cells.

    abstract::The addition of 5-azacytidine to erythroleukemic cells which were induced to differentiate with DMSO or BA altered the expression of the hemoglobins. After the addition of 5-azacytidine there was an increase in hemoglobin synthesis especially in the embryonic E2 band. The beta-globin increased in synthesis after 5-aza...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zucker RM,Decal DL,Whittington KB

    更新日期:1983-10-17 00:00:00

  • Identification and characterization of homologues of the Exocyst component Sec10p.

    abstract::The SEC10 gene product is a member of the Exocyst complex essential for exocytosis in the budding yeast Saccharomyces cerevisiae. We report here the cloning and characterization of human Sec10p (hSec10p; GenBank accession number U85946). hSec10p is a 77-kDa protein with 23% amino acid identity to yeast Sec10p and 37% ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guo W,Roth D,Gatti E,De Camilli P,Novick P

    更新日期:1997-03-10 00:00:00

  • The 5'-untranslated region of the human muscle acylphosphatase mRNA has an inhibitory effect on protein expression.

    abstract::The cDNA of the human muscle type acylphosphatase was isolated and characterized. The mRNA presents a very long 5'-untranslated region, covering the first half of the molecule: 175 bases of this part were cloned and prediction of the possible secondary structure showed that a very stable stem-loop structure could be f...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fiaschi T,Marzocchini R,Raugei G,Veggi D,Chiarugi P,Ramponi G

    更新日期:1997-11-03 00:00:00