Abstract:
:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP(C) and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP(C) interacts with ZAP-70, suggesting that PrP(C) is a component of the multimolecular signaling complex within microdomains involved in T cell activation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice Mdoi
10.1016/S0014-5793(04)00029-8keywords:
subject
Has Abstractpub_date
2004-02-27 00:00:00pages
14-8issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000298journal_volume
560pub_type
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