Prion protein is a component of the multimolecular signaling complex involved in T cell activation.

Abstract:

:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipitates with GM3 and the raft marker GM1. A strict association between PrP(C) and Fyn was revealed by scanning confocal microscopy and coimmunoprecipitation experiments. The phosphorylation protein ZAP-70 was immunoprecipitated by anti-PrP after T cell activation. These results demonstrate that PrP(C) interacts with ZAP-70, suggesting that PrP(C) is a component of the multimolecular signaling complex within microdomains involved in T cell activation.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M

doi

10.1016/S0014-5793(04)00029-8

keywords:

subject

Has Abstract

pub_date

2004-02-27 00:00:00

pages

14-8

issue

1-3

eissn

0014-5793

issn

1873-3468

pii

S0014579304000298

journal_volume

560

pub_type

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