Abstract:
:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as strong as that of tea (-)-epigallocatechin gallate and causes a significant S-phase cell-cycle arrest in both cell lines at doses higher than 100 microM. The other cysteinyl compounds do not affect normal cell cycle distribution. The gallate derivative also induces apoptosis in melanoma cells more strongly than the other derivatives and the parent (-)-epicatechin do. The gallate compound seems to trigger nuclear condensation and fragmentation, which is confirmed by DNA laddering. Interestingly, they do not induce apoptosis in keratinocytes (HaCaT).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante Mdoi
10.1016/j.febslet.2005.06.051keywords:
subject
Has Abstractpub_date
2005-08-15 00:00:00pages
4219-25issue
20eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00796-9journal_volume
579pub_type
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