Intracellular distribution of DNA methyltransferase during the cell cycle.

Abstract:

:The intracellular distribution of DNA methyltransferase has been analyzed in synchronously proliferating human cells. The localization of DNA methyltransferase was determined immunocytochemically using monoclonal antibodies directed against this enzyme. DNA methyltransferase was found to accumulate predominantly in nuclei with weak cytoplasmic staining. The DNA methyltransferase antigen was absent in early G1 phase, appeared in late G1 prior to the onset of DNA synthesis and persisted throughout S and G2 phases of the cell cycle. Mitotic cells showed a particularly strong staining intensity. These results show that DNA methyltransferase levels fluctuate during the cell cycle. This has possible implications on the stability of the DNA methylation pattern.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Vogel MC,Papadopoulos T,Müller-Hermelink HK,Drahovsky D,Pfeifer GP

doi

10.1016/0014-5793(88)80275-8

subject

Has Abstract

pub_date

1988-08-15 00:00:00

pages

9-13

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(88)80275-8

journal_volume

236

pub_type

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