Protective roles for ATM in cellular response to oxidative stress.

abstract：:The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI ...

journal_title：FEBS letters

authors： Larocque S,Brisson JR,Thérisod H,Perry MB,Caroff M

更新日期：2003-01-30 00:00:00

abstract：:A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...

journal_title：FEBS letters

authors： Christmanson L,Betsholtz C,Gustavsson A,Johansson B,Sletten K,Westermark P

更新日期：1991-04-09 00:00:00

abstract：:Members of the Trp protein family have been suggested as the structural basis of store-operated cation conductances. With this study, we provide evidence for the expression of three isoforms of Trp (hTrp1, 3 and 4) in human umbilical vein endothelial cells (HUVEC). The role of Trp proteins in store regulation of endot...

journal_title：FEBS letters

authors： Groschner K,Hingel S,Lintschinger B,Balzer M,Romanin C,Zhu X,Schreibmayer W

更新日期：1998-10-16 00:00:00

abstract：:The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physi...

journal_title：FEBS letters

authors： Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen P

更新日期：1994-03-14 00:00:00

abstract：:The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact w...

journal_title：FEBS letters

authors： Bier M,Fath S,Tschochner H

更新日期：2004-04-23 00:00:00

abstract：:Phospholipase C (PLC) was purified from squid retina. Soluble Gq alpha, membrane Gq alpha and G beta gamma were isolated from GTP gamma S-treated and light-illuminated photoreceptor membranes. The membrane Gq alpha stimulated phosphatidyl inositol-phospholipase C (PI-PLC) activity in a dose-dependent manner. Soluble G...

journal_title：FEBS letters

authors： Suzuki T,Terakita A,Narita K,Nagai K,Tsukahara Y,Kito Y

更新日期：1995-12-27 00:00:00

abstract：:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

journal_title：FEBS letters

authors： Dash D,Aepfelbacher M,Siess W

更新日期：1995-04-24 00:00:00

abstract：:The polyketide synthase associated with the biosynthesis of enediyne-containing calicheamicin contains a putative phosphopantetheinyl transferase (PPTase) domain. By cloning and expressing the C-terminal region of the polyketide synthase and in vitro phosphopantetheinylation assay, we found that the PPTase domain exhi...

journal_title：FEBS letters

authors： Murugan E,Liang ZX

更新日期：2008-04-02 00:00:00

abstract：:Regulated permeability changes have been detected in mitochondria across species. We review here their key features, with the goal of assessing whether a "permeability transition" similar to that observed in higher eukaryotes is present in other species. The recent discoveries (i) that treatment with cyclosporin A (Cs...

journal_title：FEBS letters

authors： Azzolin L,von Stockum S,Basso E,Petronilli V,Forte MA,Bernardi P

更新日期：2010-06-18 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:Different hydrophobic glycoproteins are associated to native biliary vesicles, which are the major carrier of biliary cholesterol. Some of these proteins promote cholesterol crystallization, a key step in cholesterol gallstone formation. This study was specifically conducted to identify the 130 kDa biliary vesicle-ass...

journal_title：FEBS letters

authors： Núñez L,Amigo L,Rigotti A,Puglielli L,Mingrone G,Greco AV,Nervi F

更新日期：1993-08-23 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied by pulse-chase and continuous labeling experiments in organ culture from 15-day-old rabbits in the presence of glycosylation and processing inhibitors. Monensin and brefeldin A inhibited the two proteolytic cleavages of the p...

journal_title：FEBS letters

authors： Rossi M,Maiuri L,Salvati VM,Russomanno C,Auricchio S

更新日期：1993-12-27 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...

journal_title：FEBS letters

authors： Jezek P,Modrianský M,Garlid KD

更新日期：1997-05-19 00:00:00

abstract：:The assembly of the Sm-class of uridine-rich small nuclear ribonucleoproteins (U snRNPs), albeit spontaneous in vitro, has recently been shown to be dependent on the aid of a large number of assisting factors in vivo. These factors are organized in two interacting units termed survival motor neuron (SMN)- and protein ...

journal_title：FEBS letters

authors： Neuenkirchen N,Chari A,Fischer U

更新日期：2008-06-18 00:00:00

abstract：:The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

journal_title：FEBS letters

authors： Galietta LJ,Haggie PM,Verkman AS

更新日期：2001-06-22 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:Two-photon scanning laser and confocal microscopies were used to image metabolic dynamics of single or cell populations of Saccharomyces cerevisiae strain 28033. Autofluorescence of reduced nicotinamide nucleotides, and mitochondrial membrane potential (DeltaPsim), were simultaneously monitored. Spontaneous, large-sca...

journal_title：FEBS letters

authors： Aon MA,Cortassa S,Lemar KM,Hayes AJ,Lloyd D

更新日期：2007-01-09 00:00:00

abstract：:Telomerase maintains the ends of eukaryotic chromosomes, and its activity is an important parameter correlating with the proliferative capacity of cells. We have investigated cell cycle-specific changes in telomerase activity using cultures of Desmodesmus quadricauda, a model alga naturally synchronized by light/dark ...

journal_title：FEBS letters

authors： Ševčíková T,Bišová K,Fojtová M,Lukešová A,Hrčková K,Sýkorová E

更新日期：2013-03-18 00:00:00

abstract：:The distribution of flux control coefficients of a glycolytic system in starved rat liver has been determined. The flux control coefficient profile in starved conditions is compared with normal fed conditions showing that in the former phosphofructokinase enhances more than 2-fold its flux control coefficient while gl...

journal_title：FEBS letters

authors： Torres NV,Mateo F,Meléndez-Hevia E

更新日期：1988-06-06 00:00:00

abstract：:The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

journal_title：FEBS letters

authors： Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

更新日期：1999-07-02 00:00:00

abstract：:Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separa...

journal_title：FEBS letters

authors： Dobos GJ,Wu XR,Traynor-Kaplan A

更新日期：1993-06-14 00:00:00

abstract：:Abstract Hyperhomocysteinemia has recently been identified as an important risk factor for Alzheimer's disease (AD). One of the potential mechanisms underlying harmful effects of homocysteine (Hcy) is site-specific acylation of proteins at lysine residues by homocysteine thiolactone (HCTL). The accumulation of amyloid...

journal_title：FEBS letters

authors： Khodadadi S,Riazi GH,Ahmadian S,Hoveizi E,Karima O,Aryapour H

更新日期：2012-01-20 00:00:00

abstract：:A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the '50 kDa' domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive m...

journal_title：FEBS letters

authors： Griffiths AJ,Trayer IP

更新日期：1989-01-02 00:00:00

abstract：:Ca(2+)-dependent protein kinases (CDPKs) play an essential role in plant Ca(2+)-mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress sti...

journal_title：FEBS letters

authors： Wan B,Lin Y,Mou T

更新日期：2007-03-20 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:Activated B61.SF.1 and CTLL-2 T lymphocyte clones which are strictly dependent on interleukin-2 (IL-2) for growth were used to study the activation of Na+/K+-ATPase. 50% of [3H]thymidine maximal incorporation was obtained when the extracellular concentration of Na+ or K+ was reduced to 50 or 2 mM, respectively. 'Quies...

journal_title：FEBS letters

authors： Redondo JM,López Rivas A,Fresno M

更新日期：1986-10-06 00:00:00

abstract：:Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...

journal_title：FEBS letters

authors： Heindel U,Schmidt MF,Veit M

更新日期：2003-06-05 00:00:00

abstract：:Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...

journal_title：FEBS letters

authors： Moore GA,McConkey DJ,Kass GE,O'Brien PJ,Orrenius S

更新日期：1987-11-30 00:00:00