Abstract:
:The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied by pulse-chase and continuous labeling experiments in organ culture from 15-day-old rabbits in the presence of glycosylation and processing inhibitors. Monensin and brefeldin A inhibited the two proteolytic cleavages of the precursor indicating that they are post-Golgi events as previously reported for the unique cleavage of LPH in man. The inhibition was not related to a concomitant alteration glycosylation; in fact, if trimming was blocked by MDNM the abnormal glycosylated precursor was proteolytically processed normally. Finally the use of the anti-microtubular drug colchicine strongly inhibited both cleavages and caused accumulation of the complex-glycosylated precursor form the brush border fraction indicating that proteolytic events depend on intact microtubule (transport).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rossi M,Maiuri L,Salvati VM,Russomanno C,Auricchio Sdoi
10.1016/0014-5793(93)80824-esubject
Has Abstractpub_date
1993-12-27 00:00:00pages
299-303issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80824-Ejournal_volume
336pub_type
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