The environments of Trp-248 and Trp-330 in tryptophan indole-lyase from Escherichia coli.

abstract：:Some pathogenic mutations associated with Alzheimer's disease are thought to affect structural-dynamic properties and the lateral dimerization of amyloid precursor protein (APP) in neuron membrane. Dimeric structure of APP transmembrane fragment Gln(686)-Lys(726) was determined in membrane-mimicking dodecylphosphochol...

journal_title：FEBS letters

authors： Nadezhdin KD,Bocharova OV,Bocharov EV,Arseniev AS

更新日期：2012-06-12 00:00:00

abstract：:Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...

journal_title：FEBS letters

authors： van Walraven HS,Krab K,Hagendoorn MJ,Kraayenhof R

更新日期：1985-05-06 00:00:00

abstract：:Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...

journal_title：FEBS letters

authors： Carson SD

更新日期：2000-11-03 00:00:00

abstract：:Several of the recently described leucocyte surface (glyco)-proteins with significant amino acid sequence similarity (human CD9, CD37, CD53, CD63, TAPA-1, CO-029 and R2 and several homologues of other species) are distinguished by the polypeptide chain apparently four times crossing the membrane. Although the biologic...

journal_title：FEBS letters

authors： Horejsí V,Vlcek C

更新日期：1991-08-19 00:00:00

abstract：:Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

journal_title：FEBS letters

authors： Kawade R,Kuroda D,Tsumoto K

更新日期：2020-03-01 00:00:00

abstract：:Unstimulated human fibrosarcoma cells (HT1080) constitutively secrete matrix metalloproteinase 2 (MMP 2) as a proenzyme requiring proteolytic cleavage by membrane type-1 MMP (MT1 MMP) for activation. Physiological and pharmacological stimuli induce clustering of MT1 MMP/tissue inhibitor of MMP 2 "receptors", promoting...

journal_title：FEBS letters

authors： Atkinson SJ,English JL,Holway N,Murphy G

更新日期：2004-05-21 00:00:00

abstract：:We have previously described a partial cDNA sequence encoding a RhoGAP protein, GAP25 that is homologous to the recently reported ArhGAP9 and ArhGAP12. We now describe a related new member ArhGAP15 that shares a number of domain similarities, including a pleckstrin homology (PH) domain, a RhoGAP domain and a novel mot...

journal_title：FEBS letters

authors： Seoh ML,Ng CH,Yong J,Lim L,Leung T

更新日期：2003-03-27 00:00:00

abstract：:Treatment of Chinese hamster ovary K1 cells with phosphatidylserine (PS) caused typical apoptosis with distinct morphological and biochemical features in a dose- and time-dependent manner. However, unlike camptothecin-induced apoptosis, changes in mitochondrial transmembrane potential were not observed. In addition, c...

journal_title：FEBS letters

authors： Miyato Y,Ibuki Y,Ohyama H,Yamada T,Goto R

更新日期：2001-08-24 00:00:00

abstract：:The cardiovascular system consists of many cell types with distinct embryonic origins. Cells from an Islet1 (Isl1)-expressing progenitor population make a substantial contribution to the developing heart. We reasoned that cells derived from Isl1-expressing progenitors might contribute more widely to the cardiovascular...

journal_title：FEBS letters

authors： Keenan ID,Rhee HJ,Chaudhry B,Henderson DJ

更新日期：2012-06-21 00:00:00

abstract：:Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

journal_title：FEBS letters

authors： Quest AF,Eppenberger HM,Wallimann T

更新日期：1990-03-26 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:In this report, we show for the first time that ceramide-1-phosphate (C1P) stimulates the phosphatidylinositol 3-kinase (PI3-K)/protein kinase B (PKB) pathway, which is a major mechanism whereby growth factors promote cell survival. Also, C1P induced IkappaB phosphorylation, and enhanced the DNA binding activity of th...

journal_title：FEBS letters

authors： Gómez-Muñoz A,Kong JY,Parhar K,Wang SW,Gangoiti P,González M,Eivemark S,Salh B,Duronio V,Steinbrecher UP

更新日期：2005-07-04 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arac...

journal_title：FEBS letters

authors： Krishnan LK,Jamaluddin MP

更新日期：1987-02-23 00:00:00

abstract：:Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

journal_title：FEBS letters

authors： Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

更新日期：1988-06-20 00:00:00

abstract：:We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...

journal_title：FEBS letters

authors： Miura D,Kida Y,Nojima H

更新日期：2007-05-29 00:00:00

abstract：:Periplasmic adaptor proteins are essential components of bacterial tripartite multidrug efflux pumps. Here we report the 2.35 Å resolution crystal structure of the BesA adaptor from the spirochete Borrelia burgdorferi solved using selenomethionine derivatized protein. BesA shows the archetypal linear, flexible, multi-...

journal_title：FEBS letters

authors： Greene NP,Hinchliffe P,Crow A,Ababou A,Hughes C,Koronakis V

更新日期：2013-09-17 00:00:00

abstract：:Targeting gene therapy vectors to abundant receptors on airway epithelia may allow a significant enhancement of gene delivery and thereby be of particular importance for the gene therapy of cystic fibrosis. Alpha9beta1-integrins are highly expressed throughout the human airway epithelia in vivo, irrespective of any pa...

journal_title：FEBS letters

authors： Schneider H,Harbottle RP,Yokosaki Y,Kunde J,Sheppard D,Coutelle C

更新日期：1998-06-16 00:00:00

abstract：:A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

journal_title：FEBS letters

authors： Palomäki A,Malminiemi K,Metsä-Ketelä T

更新日期：1997-06-30 00:00:00

abstract：:HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

journal_title：FEBS letters

authors： Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

更新日期：1993-10-18 00:00:00

abstract：:Particulate drug carriers, including liposomes, are rapidly removed from blood by cells of the reticuloendothelial system (RES) with resulting adverse effects on this important host defense system. In order to overcome this and other major disadvantages of liposomes, we have altered liposome composition in an effort t...

journal_title：FEBS letters

authors： Allen TM,Chonn A

更新日期：1987-10-19 00:00:00

abstract：:Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. He...

journal_title：FEBS letters

authors： Nakashima K,Yanagisawa M,Arakawa H,Taga T

更新日期：1999-08-20 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...

journal_title：FEBS letters

authors： Ghazi A,Delamourd L,Shechter E

更新日期：1986-12-15 00:00:00

abstract：:We have found that N-acetylneuraminic acid (NANA) consumes toxic hydrogen peroxide (H(2)O(2)) under physiological conditions. Close investigation of this finding revealed that NANA was oxidized by an equimolar amount of H(2)O(2) to provide its decarboxylated product, 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-oct...

journal_title：FEBS letters

authors： Iijima R,Takahashi H,Namme R,Ikegami S,Yamazaki M

更新日期：2004-03-12 00:00:00

abstract：:Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...

journal_title：FEBS letters

authors： Herrera F,Outeiro TF

更新日期：2012-01-02 00:00:00

abstract：:Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...

journal_title：FEBS letters

authors： Kiss AL,Szeltner Z,Fülöp V,Polgár L

更新日期：2004-07-30 00:00:00

abstract：:The morula and the mesenchyme blastula nuclei contained approx. 30 nuclear proteins which were preferentially released by limited digestion with DNase I, but no proteins were released from sperm nuclei. While most of the proteins released by DNase I digestion were common to the two embryonic stages, 2 and 6 proteins w...

journal_title：FEBS letters

authors： Takahashi K

更新日期：1984-04-24 00:00:00

abstract：:A single protein from human leukocytes possesses both 5-lipoxygenase and leukotriene A4 (LTA4) synthase activities. It has been reported that LTA4 production is more efficient when the enzyme utilizes arachidonic acid, than when 5-HPETE is exogenously supplied as substrate. In the present study, human leukocyte homoge...

journal_title：FEBS letters

authors： Puustinen T,Scheffer MM,Samuelsson B

更新日期：1987-06-15 00:00:00

abstract：:Homocysteine thiolactone is a product of an error-editing reaction, catalyzed by Escherichia coli and Saccharomyces cerevisiae methionyl-tRNA synthetases, which prevents incorporation of homocysteine into tRNA and protein both in vitro and in vivo. Here, homocysteine thiolactone is also shown to be synthesized by cult...

journal_title：FEBS letters

authors： Jakubowski H,Goldman E

更新日期：1993-02-15 00:00:00