Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin.


:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) from man, mouse and chicken and recognises a quite different part of the proposed triple-helical region of dystrophin (amino acids 1750-2248). The results suggest that structural homologies between actinin and dystrophin occur more than once in their central helical regions and provide experimental support for an actinin-like central rod model for dystrophin.




FEBS letters


Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE




Has Abstract


1990-10-15 00:00:00












  • Amiloride at pH 7.0 inhibits the Na(+)-driven flagellar motors of Vibrio alginolyticus but allows cell growth.

    abstract::Amiloride, a specific inhibitor for the Na(+)-driven flagellar motors of alkalophilic Bacillus, is known to inhibit secondarily the growth of alkalophiles. The motility of a marine Vibrio, V. alginolyticus, was almost completely inhibited by 2 mM amiloride either at pH 7.0 or 8.5. We found that this concentration of a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Atsumi T,Maekawa Y,Tokuda H,Imae Y

    更新日期:1992-12-14 00:00:00

  • Fluorescence ratiometric detection of ligand-induced receptor internalization using extracellular coiled-coil tag-probe labeling.

    abstract::We report a new method for the detection of ligand-induced receptor internalization by fluorescence ratiometric imaging of pH in endosomes in combination with a recently developed posttranslational labeling system based on the formation of a heterodimeric coiled-coil structure. The N-terminus of the β2-adrenergic rece...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yano Y,Matsuzaki K

    更新日期:2011-07-21 00:00:00

  • Tertiary structural models for human interleukin-6 and evaluation by a sequence-structure compatibility method and NMR experimental information.

    abstract::Tertiary structure models of Interleukin-6 were constructed using a routine prediction method based on the X-ray crystal structures of granulocyte colony-stimulating factor (GCSF) and leukemia inhibitory factor (LIF). The models were evaluated with the aid of the sequence-structure compatibility (3D-1D) method program...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sumikawa H,Fukuhara K,Suzuki E,Matsuo Y,Nishikawa K

    更新日期:1997-03-10 00:00:00

  • Tubulin and microtubule are potential targets for brain hexokinase binding.

    abstract::The metabolite-modulated association of a fraction of hexokinase to mitochondria in brain is well documented, however, the involvement of other non-mitochondrial components in the binding of the hexokinase is controversial. Now we present evidence that the hexokinase binds both tubulin and microtubules in brain in vit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wágner G,Kovács J,Löw P,Orosz F,Ovádi J

    更新日期:2001-11-30 00:00:00

  • A genetic approach to elucidating eukaryotic iron metabolism.

    abstract::Studies of mutants of the yeast Saccharomyces cerevisiae have led to the identification of genes required for high affinity iron uptake. Reduction of iron (III) outside the cell is accomplished by means of reductases encoded by FRE1 and FRE2, homologues of the gp91-phox component of the oxygen reductase of human granu...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Klausner RD,Dancis A

    更新日期:1994-11-28 00:00:00

  • Deprivation of nerve growth factor rapidly increases purine efflux from cultured sympathetic neurons.

    abstract::The efflux of [3H]purines from cultured sympathetic neurons prelabelled with [3H]adenine is accelerated 2-3-fold within hours of nerve growth factor (NGF) withdrawal and is reduced by readdition of NGF. Addition of 8-(4-chlorphenyl-thio) cAMP, which delays neurite degeneration, reduced the enhanced efflux of purines, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tolkovsky AM,Buckmaster EA

    更新日期:1989-09-25 00:00:00

  • The three-dimensional solution structure of a constrained peptidomimetic in water and in chloroform. Observation of solvent induced hydrophobic cluster.

    abstract::A large number of protein-protein interactions involved turn or loop regions. The excised linear peptides from these regions reveal complex conformational averaging. To circumvent this motional averaging and to stabilize the beta-turn conformation, extensive effort has been devoted to the design of constrained peptido...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lee MS,Gardner B,Kahn M,Nakanishi H

    更新日期:1995-02-13 00:00:00

  • Alternating purine-pyrimidine tract activates transcription from the Rouse sarcoma virus LTR lacking promoter and enhancer elements.

    abstract::The transcriptional control region of the Rouse sarcoma virus long terminal repeats (LTR) was shown to contain enhancer and promoter elements located within 200 base pairs upstream from the transcription initiation site [Cullen et al. (1985) Mol. Cell. Biol. 5, 438-447]. Deletion of these elements results in significa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krajewski WA

    更新日期:1995-01-16 00:00:00

  • AtCYP20-2 is an auxiliary protein of the chloroplast NAD(P)H dehydrogenase complex.

    abstract::AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sirpiö S,Holmström M,Battchikova N,Aro EM

    更新日期:2009-07-21 00:00:00

  • Effects of acrolein, a natural occurring aldehyde, on the anticoagulant serpin antithrombin.

    abstract::We studied the effect of acrolein, an alpha,beta-unsaturated aldehyde that causes adduct-modification of lysine, cysteine, and histidine residues, on antithrombin, a key anticoagulant serpin. Intrinsic fluorescence, functionality (anti-FXa and anti-IIa activity), heparin affinity and conformational features of plasma ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Martínez-Martínez I,Ordóñez A,Guerrero JA,Pedersen S,Miñano A,Teruel R,Velázquez L,Kristensen SR,Vicente V,Corral J

    更新日期:2009-10-06 00:00:00

  • Molecular evolution of the calmodulin gene.

    abstract::On the basis of the intron/exon organization and the intramolecular homology of DNA sequences, I propose a novel model for genesis of the calmodulin gene. A primordial calmodulin gene consisting of 51 base pairs (17 amino acids) was subjected to three-fold duplication to create modern calmodulin with four calcium-bind...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nojima H

    更新日期:1987-06-15 00:00:00

  • hOLF44, a secreted glycoprotein with distinct expression pattern, belongs to an uncharacterized olfactomedin-like subfamily newly identified by phylogenetic analysis.

    abstract::Secreted proteins are indispensable for the development and differentiation of multicellular organisms. Cloning and characterization of novel or hypothetical genes encoding these proteins are therefore inviting great incentives. Using bioinformatics tools and experimental approaches, we isolated and characterized a hu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zeng LC,Liu F,Zhang X,Zhu ZD,Wang ZQ,Han ZG,Ma WJ

    更新日期:2004-07-30 00:00:00

  • Single molecule approaches to transcription factor kinetics in living cells.

    abstract::Quantitative modeling of intracellular processes often requires information about intracellular rate constants as well as the concentrations of low abundance species in individual cells. Single molecule imaging techniques offer not only new ways for obtaining such information but also the possibilities to test model-b...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Li GW,Elf J

    更新日期:2009-12-17 00:00:00

  • Zymosan induces selective release of arachidonic acid from rabbit alveolar macrophages via stimulation of a beta-glucan receptor.

    abstract::Zymosan, which is composed primarily of alpha-mannan and beta-glucan polymers, is a well recognized activator of macrophages. The type receptor by which unopsonized zymosan induces arachidonic acid release was investigated. It was found that particulate beta-glucan and zymosan stimulated an identical dose-dependent re...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Daum T,Rohrbach MS

    更新日期:1992-09-07 00:00:00

  • Analogs of human epidermal growth factor which partially inhibit the growth factor-dependent protein-tyrosine kinase activity of the epidermal growth factor receptor.

    abstract::Three site-directed mutants of human epidermal growth factor, Leu-26----Gly, Leu-47----Ala, and Ile-23----Thr, were examined for their ability to stimulate the protein-tyrosine kinase activity of the epidermal growth factor receptor. The receptor binding affinities of the mutant growth factors were 20- to 50-fold lowe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsunami RK,Campion SR,Niyogi SK,Stevens A

    更新日期:1990-05-07 00:00:00

  • Phospholipase C and phospholipase A2 are involved in the antiviral activity of human interferon-alpha.

    abstract::Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Premecz G,Markovits A,Bagi G,Farkas T,Földes I

    更新日期:1989-06-05 00:00:00

  • A specific immunological probe for the major myelin proteolipid. Confirmation of a deletion in DM-20.

    abstract::Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Trifilieff E,Luu B,Nussbaum JL,Roussel G,Espinosa de los Monteros A,Sabatier JM,Van Rietschoten J

    更新日期:1986-03-31 00:00:00

  • Structure of chicken calcitonin predicted by partial nucleotide sequence of its precursor.

    abstract::DNA complementary to chicken ultimobranchial gland mRNA was cloned into the Pst I site of plasmid vector pBR322. A plasmid was selected by DNA-mRNA hybridization. We report here the partial nucleotide sequence of chicken calcitonin mRNA and the deduced complete amino acid sequence of chicken calcitonin. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lasmoles F,Jullienne A,Desplan C,Milhaud G,Moukhtar MS

    更新日期:1985-01-21 00:00:00

  • DNA polyintercalation: comparison of DNA binding properties of an acridine dimer and trimer.

    abstract::The DNA binding characteristics of a mono-, di- and trimeric derivative of 9-aminoacridine were studied. The length of the linking carboxamidoalkyl chains was selected to allow bis- or tris-intercalation according to the excluded-site model. Measurements of DNA unwinding angle using closed circular DNA showed that the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaugain B,Markovits J,Le Pecq JB,Roques BP

    更新日期:1984-04-24 00:00:00

  • N-acetylcysteine suppresses TNF-induced NF-kappaB activation through inhibition of IkappaB kinases.

    abstract::Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF recept...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oka S,Kamata H,Kamata K,Yagisawa H,Hirata H

    更新日期:2000-04-28 00:00:00

  • Specific binding of protein kinase CK2 catalytic subunits to tubulin.

    abstract::Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Faust M,Schuster N,Montenarh M

    更新日期:1999-11-26 00:00:00

  • Interaction between human amphipathic apolipoproteins and amyloid beta-peptide: surface plasmon resonance studies.

    abstract::Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shuvaev VV,Siest G

    更新日期:1996-03-25 00:00:00

  • The role of protein glycosylation in the control of cellular N-sialyltransferase activity.

    abstract::Protein glycosylation, which is a key post-translational event, is catalysed by the glycosyltransferase family of enzymes. There is an increasing body of evidence to suggest that these enzymes may themselves be glycosylated, possibly as an autocatalytic event. Using a novel in vitro system, we have investigated the ro...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Breen KC

    更新日期:2002-04-24 00:00:00

  • Evidence for a phosphorylation-induced conformational change in phospholamban cytoplasmic domain by CD analysis.

    abstract::Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum (SR), is described as the regulator of the Ca(2+)-ATPase pump, via its phosphorylation-dephosphorylation of Ser-16. Recently it has been shown that a direct interaction between the N-terminal hydrophilic domain of PLB and Ca(2+)-ATPase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Terzi E,Poteur L,Trifilieff E

    更新日期:1992-09-14 00:00:00

  • Torus-shaped particles formed due to intermolecular condensation of circular DNA upon interaction with synthetic tripeptide.

    abstract::The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vengerov YY,Semenov TE,Streltsov SA,Makarov VL,Khorlin AA,Gursky GV

    更新日期:1985-01-21 00:00:00

  • Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates.

    abstract::The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marais RM,Nguyen O,Woodgett JR,Parker PJ

    更新日期:1990-12-17 00:00:00

  • Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells.

    abstract::The aim of this work was to study the effect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constituti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meisse D,Van de Casteele M,Beauloye C,Hainault I,Kefas BA,Rider MH,Foufelle F,Hue L

    更新日期:2002-08-28 00:00:00

  • Electrochemical characteristics of platinum electrodes coated with cytochrome b5-phospholipid monolayers.

    abstract::Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zaba BN,Wilkinson MC,Taylor DM,Lewis TJ,Laidman DL

    更新日期:1987-03-09 00:00:00

  • Muscarinic receptor modulation of glucose-induced electrical activity in mouse pancreatic B-cells.

    abstract::Acetylcholine (1-10 microM) depolarized the membrane and stimulated glucose-induced bursts of electrical activity in mouse pancreatic B-cells. The acetylcholine effects were mimicked by muscarine while nicotine had no effect on membrane potential. Pirenzepine, an antagonist of the classical M1-type muscarinic receptor...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Santos RM,Rojas E

    更新日期:1989-06-05 00:00:00

  • Protein kinase inhibitors block the stimulation of the AMP-activated protein kinase by 5-amino-4-imidazolecarboxamide riboside.

    abstract::The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fryer LG,Parbu-Patel A,Carling D

    更新日期:2002-11-06 00:00:00