Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin.

abstract：:The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

journal_title：FEBS letters

authors： Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

更新日期：1993-07-05 00:00:00

abstract：:The lactase-phlorizin hydrolase gene was assigned to chromosome 2 by analysis of Southern blots of DNA from a panel of human-rodent cell hybrids containing characteristic sets of human chromosomes The hybridization probe used was a recently isolated cDNA clone of the human lactase-phlorizin hydrolase gene. ...

journal_title：FEBS letters

authors： Kruse TA,Bolund L,Grzeschik KH,Ropers HH,Sjöström H,Norén O,Mantei N,Semenza G

更新日期：1988-11-21 00:00:00

abstract：:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

journal_title：FEBS letters

authors： Jin H,Yang X,Liu K,Gu Q,Xu X

更新日期：2011-11-04 00:00:00

abstract：:Atg18 and Atg21 are homologous S. cerevisiae autophagy proteins. Atg18 is essential for biogenesis of Cvt vesicles and autophagosomes, while Atg21 is only essential for Cvt vesicle formation. We found that mutated Atg18-(FTTGT), which lost almost completely its binding to PtdIns3P and PtdIns(3,5)P(2), is non-functiona...

journal_title：FEBS letters

authors： Krick R,Tolstrup J,Appelles A,Henke S,Thumm M

更新日期：2006-08-21 00:00:00

abstract：:Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functio...

journal_title：FEBS letters

authors： Krause K,Kilbienski I,Mulisch M,Rödiger A,Schäfer A,Krupinska K

更新日期：2005-07-04 00:00:00

abstract：:A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...

journal_title：FEBS letters

authors： Zlatanov IV,Foley M,Birmingham J,Garland PB

更新日期：1987-09-28 00:00:00

abstract：:All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

journal_title：FEBS letters

authors： Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

更新日期：1996-10-21 00:00:00

abstract：:Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...

journal_title：FEBS letters

authors： Paumi CM,Pickin KA,Jarrar R,Herren CK,Cowley ST

更新日期：2012-03-23 00:00:00

abstract：:Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell-free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli beta-galactosidase containing the last four sense codons of Drosophila hsp70 at its 3'-end was constructed. This mRNA is efficiently translated ...

journal_title：FEBS letters

authors： Denisenko ON,Yarchuk OB

更新日期：1989-04-24 00:00:00

abstract：:Upon in vitro translation of mRNAs from slow (soleus) muscles of the mouse a hitherto undescribed translation product has been detected that was absent from fast skeletal muscles and was termed p19/6.8 according to its position in 2-dimensional gels. mRNA for p19/6.8 was also found in the ventricle of the heart. p19/6...

journal_title：FEBS letters

authors： Kluxen FW

更新日期：1988-03-28 00:00:00

abstract：:A stabilization of both the precursor and the mature beta 1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt-4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differential effects of protein synthesis inhibitors are reported since the slow degradat...

journal_title：FEBS letters

authors： Vekeman S,Jaspers M,Cassiman JJ

更新日期：1993-07-26 00:00:00

abstract：:MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...

journal_title：FEBS letters

authors： Miao LJ,Huang SF,Sun ZT,Gao ZY,Zhang RX,Liu Y,Wang J

更新日期：2013-05-02 00:00:00

abstract：:Human kallikrein 8 (KLK8) is a member of the human kallikrein gene family of serine proteases, and its protein, hK8, has recently been suggested to serve as a new ovarian cancer marker. To gain insights into the physiological role of hK8, the active recombinant enzyme was obtained in a pure state for biochemical and e...

journal_title：FEBS letters

authors： Rajapakse S,Ogiwara K,Takano N,Moriyama A,Takahashi T

更新日期：2005-12-19 00:00:00

abstract：:The function of the amino- and carboxyl-terminal domains of the yeast plasma membrane H+-ATPase have been investigated by constructing deletions in vitro and selectively expressing the mutant enzymes in vivo. The first 27 amino acids are dispensable but deletion of a further 33 amino acids greatly decreases the appear...

journal_title：FEBS letters

authors： Portillo F,de Larrinoa IF,Serrano R

更新日期：1989-04-24 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...

journal_title：FEBS letters

authors： Holck A,Kleppe K

更新日期：1985-06-03 00:00:00

abstract：:Translocation of folded proteins by the Tat system of Escherichia coli is believed to rely on the presence of phosphatidylethanolamine (PE) and the negatively charged phospholipids cardiolipin (CL) and phosphatidylglycerol (PG). Here, we show that while PE is indeed essential for activity, the Tat system is fully func...

journal_title：FEBS letters

authors： Rathmann C,Schlösser AS,Schiller J,Bogdanov M,Brüser T

更新日期：2017-09-01 00:00:00

abstract：:Red blood cells from patients homozygotes for hemoglobin S (HbS) have been studied using a computer-controlled microspectrophotometer, which allows measurements of spectra and dynamics to be undertaken in a single erythrocyte. Complete photodissociation of HbCO results in polymerization of intracellular deoxyhemoglobi...

journal_title：FEBS letters

authors： Coletta M,Alayash AI,Wilson MT,Benedetti PA,Evangelista V,Brunori M

更新日期：1988-08-15 00:00:00

abstract：:Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to ...

journal_title：FEBS letters

authors： Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaM

更新日期：1991-03-11 00:00:00

abstract：:A covalent complex formed by bacterial tRNAs and prothymosin alpha, an abundant acidic nuclear protein involved in proliferation of mammalian cells, upon production of the recombinant rat protein in Escherichia coli cells was studied. Several tRNA attachment sites were identified in the prothymosin alpha molecule usin...

journal_title：FEBS letters

authors： Lukashev DE,Chichkova NV,Vartapetian AB

更新日期：1999-05-21 00:00:00

abstract：:The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independe...

journal_title：FEBS letters

authors： Cheek TR,O'Sullivan AJ,Moreton RB,Berridge MJ,Burgoyne RD

更新日期：1990-06-18 00:00:00

abstract：:Activities of Escherichia coli DNA polymerase-I were examined in the presence of the anti-tumor drug cis-diaminedichloroplatinum(II) and its inactive geometric isomer trans-diaminedichloroplatinum(II). The trans-isomer did not inhibit the enzyme activity. The anti-tumor drug, on the other hand, retarded the enzyme in ...

journal_title：FEBS letters

authors： Duman RK,Heath RT,Bose RN

更新日期：1999-07-16 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

journal_title：FEBS letters

authors： Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

更新日期：1998-07-24 00:00:00

abstract：:While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation u...

journal_title：FEBS letters

authors： Wurm S,Paar C,Sonnleitner A,Sonnleitner M,Höglinger O,Romanin C,Wechselberger C

更新日期：2004-05-21 00:00:00

abstract：:Cyclin B is a central regulator of transition from the G(2) phase of the cell cycle to mitosis. In mammalian cells two B-type cyclins have been characterised, cyclin B1 and B2. Both are expressed with a maximum in G(2) and their synthesis is mainly regulated on the transcriptional level. We show that a single cell cyc...

journal_title：FEBS letters

authors： Wasner M,Tschöp K,Spiesbach K,Haugwitz U,Johne C,Mössner J,Mantovani R,Engeland K

更新日期：2003-02-11 00:00:00

abstract：:It has long been known from fluorescence recovery after photobleaching experiments that the mobility of most cell surface receptors is much smaller than expected for free diffusion of proteins in a fluid lipid bilayer. Single-particle tracking experiments are currently revealing the complexity of the constraints to fr...

journal_title：FEBS letters

authors： Cherry RJ,Smith PR,Morrison IE,Fernandez N

更新日期：1998-06-23 00:00:00

abstract：:A variety of plant tissues contain an uncoupling mitochondrial protein (PUMP), recently described and characterized by our group. In this study we show that the inhibition of PUMP activity in potato tuber mitochondria significantly increases mitochondrial H2O2 generation, while PUMP substrates, such as linoleic acid, ...

journal_title：FEBS letters

authors： Kowaltowski AJ,Costa AD,Vercesi AE

更新日期：1998-03-27 00:00:00

abstract：:We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...

journal_title：FEBS letters

authors： Carrì MT,Ferri A,Battistoni A,Famhy L,Gabbianelli R,Poccia F,Rotilio G

更新日期：1997-09-08 00:00:00