Monoclonal antibody evidence for structural similarities between the central rod regions of actinin and dystrophin.

Abstract:

:A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) from man, mouse and chicken and recognises a quite different part of the proposed triple-helical region of dystrophin (amino acids 1750-2248). The results suggest that structural homologies between actinin and dystrophin occur more than once in their central helical regions and provide experimental support for an actinin-like central rod model for dystrophin.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Nguyen TM,Ellis JM,Ginjaar IB,van Paassen MM,van Ommen GJ,Moorman AF,Cartwright AJ,Morris GE

doi

10.1016/0014-5793(90)80460-z

subject

Has Abstract

pub_date

1990-10-15 00:00:00

pages

109-12

issue

1-2

eissn

0014-5793

issn

1873-3468

journal_volume

272

pub_type

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