Abstract:
:The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independent of external Ca2+. This rise in [Ca2+]i triggered little secretion. Single cell measurements of [Ca2+]i showed that most cells responded with a large (greater than 200 nM) rise in [Ca2+]i, whereas a minority failed to respond. The latter, whose caffeine-sensitive store was empty, buffered a Ca2+ load induced by a depolarizing stimulus more effectively than those whose store was full. The caffeine-sensitive store in bovine chromaffin cells may be involved in Ca2+ homeostasis rather than in triggering exocytosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Cheek TR,O'Sullivan AJ,Moreton RB,Berridge MJ,Burgoyne RDdoi
10.1016/0014-5793(90)81514-osubject
Has Abstractpub_date
1990-06-18 00:00:00pages
91-5issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81514-Ojournal_volume
266pub_type
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