Abstract:
:Temporary inhibition of the cysteine proteinases papain and cathepsin L was observed with several hairpin loop mutants of recombinant chicken cystatin at enzyme concentrations above nanomolar. Kinetic modelling of inhibition data, gel electrophoresis and amino acid sequencing revealed that reappearance of papain activity is due to selective cleavage of the Gly(9)-Ala10 bond in the N-terminal binding area of the chicken cystatin variants, resulting in truncated inhibitors of lower affinity. Cleavage of the same bond by contaminating papaya proteinase IV was ruled out by previous purification of papain and suitable control experiments. According to the proposed kinetic model, cleavage occurs within the enzyme-inhibitor complex with first order rate constants ktemp of 2.3 x 10(-3) up to 5 x 10(-1) s-1. A similar ktemp/Km ratio was found for all mutants (0.7 x 10(6)-2.1 x 10(6) s-1.M-1); it is almost identical with the kcat/Km ratio of the peptide substrate Z-Phe-Arg-NHMec. These results suggest that distorted contacts of one of the hairpin loops affect binding of the N-terminal contact area in a way that covalent interaction of the Gly(9)-Ala10 bond with the active-site Cys residue of papain can occur and the bond is cleaved in a substrate-like manner.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Machleidt W,Nägler DK,Assfalg-Machleidt I,Stubbs MT,Fritz H,Auerswald EAdoi
10.1016/0014-5793(95)00174-8subject
Has Abstractpub_date
1995-03-20 00:00:00pages
185-90issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00174-8journal_volume
361pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.028
更新日期:2009-01-05 00:00:00
abstract::The lateral diffusion of BK(Ca) channels was previously shown to be highly 'confined' in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BK(Ca) channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.05.069
更新日期:2011-07-21 00:00:00
abstract::Nitrogenase is a two-component metalloenzyme that catalyzes a MgATP hydrolysis driven reduction of substrates. Aluminum fluoride plus MgADP inhibits nitrogenase by stabilizing an intermediate of the on-enzyme MgATP hydrolysis reaction. We report here the redox properties and electron paramagnetic resonance (EPR) signa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00827-8
更新日期:1998-07-31 00:00:00
abstract::Three single-stranded RNA transcripts synthesized in vitro by a virion-associated RNA polymerase of bacteriophage phi 6 were sequenced at their 5'- and 3'-termini. The sequences agreed with those of the + strands of the 3 double-stranded RNA segments [FEBS Lett. (1982) 141,111-115]. The results show that the transcrip...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80815-6
更新日期:1982-11-08 00:00:00
abstract::Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.028
更新日期:2015-12-21 00:00:00
abstract::In this study, we have identified 876 polymorphism sites in 145 complete or partial genomes of SARS-CoV available in the NCBI GenBank. One hundred and seventy-four of these sites existed in two or more SARS-CoV genome sequences. According to the sequence polymorphism, all SARS-CoVs can be divided into three groups: (I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.075
更新日期:2005-09-12 00:00:00
abstract::Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80723-3
更新日期:1988-04-11 00:00:00
abstract::The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3mM hydrogen peroxide (H(2)O(2)) under non-growth conditions. H(2)O(2)-induced protein carbonylation w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.030
更新日期:2006-12-11 00:00:00
abstract::The determination of protein structure by NMR is restricted at molecular masses above 10 kDa by overlapping resonances. One way of overcoming this problem is to label the protein with 15N. The conventional way to record 15N spectra is to use heteronuclear multiple-quantum coherence. We present here an alternative appr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81124-x
更新日期:1989-09-25 00:00:00
abstract::The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80753-5
更新日期:1985-06-03 00:00:00
abstract::The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions ap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)80622-4
更新日期:1983-05-08 00:00:00
abstract::Caloric restriction (CR) is the most compelling example of lifespan extension by external manipulation. Although the molecular mechanisms remain unknown, the theory of hormesis has been invoked to explain the life promoting effects of CR. Hormesis is defined as the beneficial effects of low intensity stressor on a cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.017
更新日期:2005-12-19 00:00:00
abstract::Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.09.051
更新日期:2006-10-30 00:00:00
abstract::Calpain, a Ca2+-dependent biomodulator, alters the properties of substrate proteins by cleaving them at a limited number of specific sites. Recent studies of the structure-function relationship of calpain and X-ray analysis of its Ca2+-binding domain have revealed hitherto unknown features of the regulation of calpain...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(98)00856-4
更新日期:1998-08-14 00:00:00
abstract::We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00926-0
更新日期:1998-08-28 00:00:00
abstract::The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01013-3
更新日期:1999-08-20 00:00:00
abstract::Cellular homeostasis is linked tightly to mitochondrial functions. Some damage to mitochondrial proteins and nucleic acids can lead to the depolarization of the inner mitochondrial membrane, thereby sensitizing impaired mitochondria for selective elimination by autophagy. Mitochondrial dysfunction is one of the key as...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.02.060
更新日期:2010-04-02 00:00:00
abstract::Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using N...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01506-3
更新日期:1998-12-04 00:00:00
abstract::The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80557-j
更新日期:1991-05-20 00:00:00
abstract::H/D isotope exchange effects on P680+. reduction by Yz and electron abstraction from the water oxidizing complex (WOC) in redox state S3 by YZOX were analyzed in PS II core complexes from spinach by measurements of laser flash induced absorption changes at 820 nm and 355 nm. The results obtained reveal: (1) the rate o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01433-0
更新日期:1996-01-08 00:00:00
abstract::By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00975-2
更新日期:1998-08-28 00:00:00
abstract::Regulatory protein kinases are involved in various cellular processes such as proliferation, differentiation, and apoptosis. Using cDNA differential display, we identified MOK, a member of the mitogen-activated protein kinase superfamily, as one of the genes induced by a caudal-related homeobox transcription factor, C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.070
更新日期:2004-08-27 00:00:00
abstract::Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02191-3
更新日期:2001-03-09 00:00:00
abstract::The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80522-1
更新日期:1970-12-01 00:00:00
abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01032-1
更新日期:1998-09-11 00:00:00
abstract::It is increasingly clear that microRNAs play a crucial role in tumorigenesis. Recently, emerging evidence suggested that miR-26a is aberrantly expressed in tumor tissues. In our study, frequent down-regulation of miR-26a was observed in 10 human bladder cancer tissues. Forced expression of miR-26a in the bladder cance...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.06.021
更新日期:2013-08-02 00:00:00
abstract::There is considerable epidemiological evidence that tea drinking lowers the risk of heart disease. However, the mechanism by which tea can be protective is unknown. Hamsters were fed a normal or high cholesterol diet for 2 weeks and drank green or black tea ad libitum. The plasma lipid profile was significantly improv...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00880-1
更新日期:1998-08-14 00:00:00
abstract::The results of the present investigation relate the effects of the nutritional state and administration of clofibric acid (CLA), a hypolipidaemic drug and peroxisomal proliferator, on phosphatidylethanolamine (PE) synthesis in rat liver and fatty acid metabolism. Fasting and CLA treatment of animals causes an increase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00960-0
更新日期:1998-08-28 00:00:00
abstract::The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.05.015
更新日期:2015-07-08 00:00:00
abstract::We identified cDNAs coding for homologues to tetrapod prion proteins (PrPs) in Atlantic salmon (Salmo salar) and Japanese pufferfish (Fugu rubripes), which were termed 'similar to PrPs' (stPrPs). Besides significant sequence homologies the fish stPrPs display characteristic structural features in common with tetrapod ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00149-2
更新日期:2003-03-13 00:00:00