Abstract:
:The contents of sterol derivatives (sterols + esters, glycosides and acylated glycosides) have been determined using thin layer chromatography in leaves and bulky and organs from carrots (Daucus carota L.) potato (Solanum tuberosum L.), onion (Allium cepa L.) two varieties of radish (Raphanus sativus L.) and Gentiana purpurea L. plants. Ether-soluble lipids of leaves contain 2.8-5.2%, those of storage tissue 3.1-8.9% sterol derivatives. The fractionation of lettuce (Lactuca sativa L., Burpee's Ruby Red) leaf homogenates indicates steryl glycosides to be accumulated in a microsomal fraction.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Eichenberger W,Grob ECdoi
10.1016/0014-5793(70)80522-1keywords:
subject
Has Abstractpub_date
1970-12-01 00:00:00pages
177-180issue
3eissn
0014-5793issn
1873-3468pii
0014579370805221journal_volume
11pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The present study reported the isolation and characterization of a novel human secreted protein, named as hPAP21 (human protease-associated domain-containing protein, 21 kDa), encoded by the hypothetical gene chromosome 2 open reading frame 7 (C2orf7) that contains signal peptide in its N-terminus, without transmembra...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.039
更新日期:2004-10-22 00:00:00
abstract::The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81341-i
更新日期:1992-10-05 00:00:00
abstract::Antigen-induced B cell activation requires mobilization of the Ca(2+) second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.03.057
更新日期:2012-05-21 00:00:00
abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00
abstract::Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12059
更新日期:2016-02-01 00:00:00
abstract::Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80934-m
更新日期:1993-08-16 00:00:00
abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.07.036
更新日期:2013-09-17 00:00:00
abstract::Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.03.017
更新日期:2014-05-02 00:00:00
abstract::Bromodomains present in Brd4 and other chromatin proteins interact with acetylated histones to regulate transcription and cell growth. To study Brd4-chromatin interactions in vivo, histone H4 tail peptides were fused to a synthetic protein transduction domain (PTD) derived from the human immunodeficiency virus Tat and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.044
更新日期:2008-04-30 00:00:00
abstract::Activities of Escherichia coli DNA polymerase-I were examined in the presence of the anti-tumor drug cis-diaminedichloroplatinum(II) and its inactive geometric isomer trans-diaminedichloroplatinum(II). The trans-isomer did not inhibit the enzyme activity. The anti-tumor drug, on the other hand, retarded the enzyme in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00800-5
更新日期:1999-07-16 00:00:00
abstract::Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.084
更新日期:2004-11-05 00:00:00
abstract::Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80777-e
更新日期:1992-09-14 00:00:00
abstract::We studied the effect of acrolein, an alpha,beta-unsaturated aldehyde that causes adduct-modification of lysine, cysteine, and histidine residues, on antithrombin, a key anticoagulant serpin. Intrinsic fluorescence, functionality (anti-FXa and anti-IIa activity), heparin affinity and conformational features of plasma ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.07.062
更新日期:2009-10-06 00:00:00
abstract::Low plasma homoarginine has emerged as a risk marker for cardiovascular disease. We exploited cells of a patient with a rare inborn error of metabolism to explore potential pathways of homoarginine synthesis, using stable isotopes and mass spectrometry. Control lymphoblasts, as opposed to lymphoblasts from an arginine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.020
更新日期:2012-10-19 00:00:00
abstract::The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81229-8
更新日期:1988-08-01 00:00:00
abstract::The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.08.003
更新日期:2015-11-14 00:00:00
abstract::The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80233-8
更新日期:1987-07-27 00:00:00
abstract::Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12620
更新日期:2017-04-01 00:00:00
abstract::Mutations of the cysteines (positions 668 and 714) were generated in the IF2 C domain of Bacillus stearothermophilus translation initiation factor IF2. The corresponding proteins were characterized functionally and structurally. Most (yet not all) amino acid replacements at both positions resulted in severe reduction ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01280-6
更新日期:1999-10-15 00:00:00
abstract::PGI is a housekeeping gene encoding phosphoglucose isomerase (PGI) a glycolytic enzyme that also functions as a cytokine (autocrine motility factor (AMF)/neuroleukin/maturation factor) upon secretion from the cell and binding to its 78 kDa seven-transmembrane domain receptor (gp78/AMF-R). PGI contains a CXXC motif, ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-6793(02)03773-0
更新日期:2003-01-16 00:00:00
abstract::In several reactions catalyzed by highly purified peptide elongation factor 1 from rabbit reticulocytes, GTP may be fully replaced by CTP but not by ATP or UTP. This holds true for the factor-dependent binding of aminoacyl-tRNA to ribosomes, GTPase activity, GTP-dependent autophosphorylation of the factor protein and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80992-8
更新日期:1984-11-05 00:00:00
abstract::The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81247-j
更新日期:1992-04-20 00:00:00
abstract::We have expressed in E. coli segments of the rod portion of rabbit skeletal fast muscle myosin and compared physical properties of two different species, LMM-30 and LMM-30C'. LMM-30 consists of 263 amino acids including the original C-terminus of myosin heavy chain. LMM-30C' is colinear with LMM-30, but is devoid of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80349-8
更新日期:1991-04-09 00:00:00
abstract::By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00975-2
更新日期:1998-08-28 00:00:00
abstract::The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80720-n
更新日期:1991-07-08 00:00:00
abstract::Retinoic acid receptor-related orphan receptor-alpha (RORalpha) is a nuclear orphan receptor. Adenovirus-mediated overexpression of RORalpha1 and RORalpha4 suppressed tumor necrosis factor-alpha (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01502-3
更新日期:2004-01-16 00:00:00
abstract::The small GTPase Rho1 plays an essential role in controlling the organization of the actin cytoskeleton and synthesis of the cell wall in the fission yeast Schizosaccharomyces pombe. Here we studied the role of Rho5 whose primary structure is very similar to that of Rho1. It was found that elevated expression of Rho5 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.031
更新日期:2005-09-26 00:00:00
abstract::Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80374-5
更新日期:1987-08-31 00:00:00
abstract::Anillin is a type of actin filament cross-linking protein that stabilizes the actin-based contractile ring during cytokinesis. To elucidate the underlying intermolecular interactions between actin filaments and anillin, we utilized total internal reflection fluorescence microscopy (TIRFM) and high-speed atomic force m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13720
更新日期:2020-04-01 00:00:00
abstract::EVH1 (enabled VASP (vasodilator-stimulated protein) homology 1)/WH1 (WASP (Wiskott-Aldrich syndrome protein) homology 1) domains, present in Ena VASP and WASP, are protein interaction modules specialised in binding proline-rich ligands. An EVH1/WH1 domain is here identified in the recently cloned SMIF protein, a key p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02751-5
更新日期:2002-05-22 00:00:00