Abstract:
:By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes in their single channel conductance. Besides this effect on the main, long-lived open state, lowering the pH also suppressed the spontaneous transitions of channels to another distinct short-lived open state. The inhibitory effect on the opening kinetics was particularly visible in two mutants (K16Q and E109Q) in which transitions to the short-lived open state are enhanced by the mutations themselves at pH 7.2. On the other hand, the R124Q mutant responded to acidic pH by an increased gating to the short-lived open state. The results suggest that acidic pH stabilizes a closed state of OmpC porin, and that the pH sensitivity might be conferred in part by R124, but not by K16 or E109.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Liu N,Delcour AHdoi
10.1016/s0014-5793(98)00975-2subject
Has Abstractpub_date
1998-08-28 00:00:00pages
160-4issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00975-2journal_volume
434pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81055-x
更新日期:1988-12-05 00:00:00
abstract::Tumor suppressor WW domain-containing oxidoreductase (WWOX) is depleted in various cancer types. Here we report that WWOX is modified by small ubiquitin-like modifier (SUMO) proteins and represses DU145 prostate cancer tumorigenesis in a SUMOylation-dependent manner. Ectopic WWOX was shown to associate with SUMO2/3 or...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.028
更新日期:2015-12-21 00:00:00
abstract::A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00962-9
更新日期:1997-08-25 00:00:00
abstract::Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03832-2
更新日期:2003-01-16 00:00:00
abstract::cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81453-x
更新日期:1989-07-17 00:00:00
abstract::The red cell membrane is comprised of a lipid bilayer studded with transmembrane proteins, and laminated by a protein network, the membrane skeleton, at the surface of the inner monolayer. The erythrocyte owes its mechanical properties to the membrane skeleton. Hereditary spherocytosis, hereditary elliptocytosis or po...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(95)00460-q
更新日期:1995-08-01 00:00:00
abstract::We have reconstituted thin filaments comprising pyrene-labelled actin (pyr-actin), tropomyosin (Tm) and cardiac troponin (cTn). cTn was isolated in two defined phosphorylation states; completely dephosphorylated on all subunits and with only the cTnI subunit bisphosphorylated. The thin filament was saturated with cTn ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00274-8
更新日期:1996-04-08 00:00:00
abstract::2-thiouracil (TU), an established antithyroid drug and melanoma-seeker, was found to selectively inhibit neuronal nitric oxide synthase (nNOS) in a competitive manner (K(i)=20 microM), being inactive on the other NOS isoforms. The drug apparently interfered with the substrate- and tetrahydrobiopterin (BH(4))-binding t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02194-3
更新日期:2000-11-24 00:00:00
abstract::Magainins are a family of antimicrobial peptides present in the skin extracts of Xenopus laevis. Both magainin-1 and -2 do not have any significant effect on the activity of protein kinase C (PKC). Magainin-2 was found to be readily phosphorylated by PKC to 0.5 mol 32P/mol of peptide. Neither magainin-1, which has a s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80307-5
更新日期:1990-07-02 00:00:00
abstract::Platelet-activating factor (PAF) is a phospholipid, intermediate in its structure between diacyl- and lysophospholipids. The reorientation of this highly bioactive compound to the inner membrane layer of human erythrocytes after its primary incorporation into the outer membrane layer has been studied. Reorientation wa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80427-6
更新日期:1986-03-31 00:00:00
abstract::To elucidate hydroxyapatite-protein interaction, mutant human lysozymes in which the surface charge was modified by site-directed mutagenesis were used. Five mutant human lysozymes (K1A, K13A, K33A, R10A, R14A) were expressed in yeast. The chromatographic behavior of these lysozymes was studied with a HPLC hydroxyapat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01621-9
更新日期:1998-01-30 00:00:00
abstract::The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.03.020
更新日期:2013-05-02 00:00:00
abstract::An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.04.043
更新日期:2011-06-06 00:00:00
abstract::Galanin mediates diverse physiological functions in digestive, endocrine, and central nervous systems through G-protein-coupled receptors. Two galanin receptors have been cloned but the gene structures are unknown. We report genomic and cDNA cloning of the mouse GalR1 galanin receptor and demonstrate that the coding s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00695-9
更新日期:1997-07-14 00:00:00
abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12247
更新日期:2016-07-01 00:00:00
abstract::The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01309-7
更新日期:2003-12-18 00:00:00
abstract::Whether all inflammatory agents activate nuclear transcription factors NF-kappaB and activated protein-1 (AP-1) through the same mechanism is not known. We examined the effect of the phosphatidylinositol-3-kinase (PI-3K) inhibitor wortmannin on the activation of NF-kappaB and AP-1 by different inflammatory agents. Wor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01501-5
更新日期:2000-05-04 00:00:00
abstract::The 5 alpha-reductase activities in human prostatic nuclei and microsomes were compared. The activities in both subcellular fractions were identical with respect to pH dependence, heat inactivation and Michaelis constants for NADPH and testosterone. Subcellular distribution studies using DNA and nicotinamide mononucle...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80912-1
更新日期:1985-06-17 00:00:00
abstract::In isolated rat liver perfused at constant flow with erythrocyte-free Krebs-Henseleit bicarbonate buffer containing 5 mM glucose and 2 mM lactate, perivascular stimulation of the hepatic nerves caused a rapid decrease of oxygen uptake, a decrease of the periportal and, after a transient rise, of the perivenous tissue ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80844-3
更新日期:1984-02-13 00:00:00
abstract::The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80758-b
更新日期:1990-05-07 00:00:00
abstract::Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00990-2
更新日期:1994-10-03 00:00:00
abstract::Expression of calcyclin in different cell lines and mouse tissues was determined with polyclonal antibodies raised against calcyclin from Ehrlich ascites tumour (EAT) cells. The protein was detected in mouse skeletal and cardiac muscle, in lung, kidney and spleen, and was especially enriched in mouse smooth muscle as ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81026-9
更新日期:1989-08-28 00:00:00
abstract::Mitochondrial dihydroorotate dehydrogenase (DHOdehase; EC 1.3.99.11) is a target of anti-proliferative, immunosuppressive and anti-parasitic agents. Here, redoxal, (2,2'-[3,3'-dimethoxy[1, 1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid, was studied with isolated mitochondria and the purified recombinant human and rat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01117-0
更新日期:2000-02-04 00:00:00
abstract::The predominance of oxidative stress in many tumour cell environments provides a means to selectively target these cells via protein oxidation. The zinc fingers of transcription factors utilise cysteine thiols for structural zinc coordination. Redox control of DNA binding regulates transcription and therefore the over...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03890-5
更新日期:2003-01-30 00:00:00
abstract::The YukD protein in Bacillus subtilis was identified in a hidden Markov model (HMM) search as being related in sequence to ubiquitin. By solving the crystal structure we show that YukD adopts a fold that is most closely related to ubiquitin, yet has the shortest C-terminal tail of all known ubiquitin-like proteins. Th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.002
更新日期:2005-07-04 00:00:00
abstract::A unique Cu,Zn-SOD was found and isolated from plaice Paralichthys olivaceus skin. Surprisingly, the properties of purified fish skin SOD were very different from those of SOD from other sources reported so far. The purified SOD was composed of four same subunits of 16 kDa and the molecular weight of the native SOD wa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00084-m
更新日期:1995-02-27 00:00:00
abstract::Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.034
更新日期:2010-06-03 00:00:00
abstract::The control of physiological processes requires the regulation and coordination of many different signals and is determined in part by the activation and repression of expression of specific target genes. RIP140 is a ligand dependent coregulator of many nuclear receptors that influence such diverse processes as muscle...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.11.017
更新日期:2008-01-09 00:00:00
abstract::The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00974-0
更新日期:1998-09-04 00:00:00
abstract::Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80006-g
更新日期:1993-03-01 00:00:00