Abstract:
:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-4218]. Alternate cDNA clones were isolated which code for a protein slightly different within the aminoterminal part from the known amino acid sequence. These alternate cDNAs contain the sequence of a peptide identified in the isoform I beta of cGMP-dependent protein kinase. Northern blot analysis of poly(A)+ RNA from bovine trachea smooth muscle indicated the presence of two different mRNA species of about 6.2 kb.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wernet W,Flockerzi V,Hofmann Fdoi
10.1016/0014-5793(89)81453-xsubject
Has Abstractpub_date
1989-07-17 00:00:00pages
191-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81453-Xjournal_volume
251pub_type
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