The C-terminal domain of the human EP4 receptor confers agonist-induced receptor desensitization in a receptor hybrid with the rat EP3beta receptor.

abstract：:The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

journal_title：FEBS letters

authors： He X,Bell AF,Tonge PJ

更新日期：2003-08-14 00:00:00

abstract：:Phospholamban (PLB), an integral membrane protein of cardiac sarcoplasmic reticulum (SR), is described as the regulator of the Ca(2+)-ATPase pump, via its phosphorylation-dephosphorylation of Ser-16. Recently it has been shown that a direct interaction between the N-terminal hydrophilic domain of PLB and Ca(2+)-ATPase...

journal_title：FEBS letters

authors： Terzi E,Poteur L,Trifilieff E

更新日期：1992-09-14 00:00:00

abstract：:The relationship between glutathione deficiency, glycogen metabolism and ascorbate synthesis was investigated in isolated murine hepatocytes. Glutathione deficiency caused by various agents increased ascorbate synthesis with a stimulation of glycogen breakdown. Increased ascorbate synthesis from UDP-glucose or gulonol...

journal_title：FEBS letters

authors： Braun L,Csala M,Poussu A,Garzó T,Mandl J,Bánhegyi G

更新日期：1996-06-17 00:00:00

abstract：:In photosystem II (PSII) the probability that energy absorbed by core antenna chlorophyll (Chl) is transferred to the reaction center (RC) is extremely high. Although close proximity between antenna Chl ensures a high transfer efficiency, relative pigment orientation can fractionally modify it. This level of refinemen...

journal_title：FEBS letters

authors： Vasil'ev S,Shen JR,Kamiya N,Bruce D

更新日期：2004-03-12 00:00:00

abstract：:Retention of intron 8 in alternative HER-2 mRNA generates an inhibitory secreted ligand, Herstatin, with a novel receptor-binding domain (RBD) encoded by the intron. This study examines binding interactions with several receptors and investigates sequence variations in intron 8. The RBD, expressed as a peptide, binds ...

journal_title：FEBS letters

authors： Shamieh LS,Evans AJ,Denton MC,Clinton GM

更新日期：2004-06-18 00:00:00

abstract：:Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...

journal_title：FEBS letters

authors： Seitz C,Ameres S,Forkmann G

更新日期：2007-07-24 00:00:00

abstract：:The stomach form of alcohol dehydrogenase has been structurally evaluated by peptide analysis covering six separate regions of the rat enzyme. Overall, this new structure differs widely (32-40% residue differences) from the structures of three classes of alcohol dehydrogenase characterized before from the same species...

journal_title：FEBS letters

authors： Parés X,Moreno A,Cederlund E,Höög JO,Jörnvall J

更新日期：1990-12-17 00:00:00

abstract：:Methane monooxygenase (MMO) catalyses the biological transformation of methane to methanol at a binuclear iron site. Guided by the three-dimensional structure of the R2 protein of E. coli ribonucleotide reductase (RNR), we have aligned the sequences of two different MMOs with the sequences of the iron coordinating fou...

journal_title：FEBS letters

authors： Nordlund P,Dalton H,Eklund H

更新日期：1992-08-03 00:00:00

abstract：:In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

journal_title：FEBS letters

authors： El Sayegh TY,Kapus A,McCulloch CA

更新日期：2007-01-23 00:00:00

abstract：:A human insulinoma cDNA (PC2) that encodes a protein homologous to the Kex2/subtilisin-like proteinases has recently been described [1990, J. Biol. Chem. 265, 2997-3000]. In order to characterise the associated proteinase activity, mRNA encoding PC2 was synthesised in vitro and microinjected into Xenopus oocytes. The ...

journal_title：FEBS letters

authors： Shennan KI,Smeekens SP,Steiner DF,Docherty K

更新日期：1991-06-24 00:00:00

abstract：:We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...

journal_title：FEBS letters

authors： Xu W,Ozdener F,Li JG,Chen C,de Riel JK,Weinstein H,Liu-Chen LY

更新日期：1999-03-26 00:00:00

abstract：:This work describes the molecular cloning of a variant isoform of the low-affinity levocabastine-sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS-7 cells, this non-functional neu...

journal_title：FEBS letters

authors： Botto JM,Sarret P,Vincent JP,Mazella J

更新日期：1997-01-03 00:00:00

abstract：:WW domains harbor substrates containing proline-rich motifs, but the substrate specificity and binding mechanism remain elusive for those WW domains less amenable for structural studies, such as human WWP2 (hWWP2). Herein we have employed multiple techniques to investigate the second WW domain (WW2) in hWWP2. Our resu...

journal_title：FEBS letters

authors： Jiang J,Wang N,Jiang Y,Tan H,Zheng J,Chen G,Jia Z

更新日期：2015-07-08 00:00:00

abstract：:The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...

journal_title：FEBS letters

authors： Torda AE,Mabbutt BC,van Gunsteren WF,Norton RS

更新日期：1988-11-07 00:00:00

abstract：:The unfolded protein response (UPR)--the endoplasmic reticulum stress response--is found in various pathologies including ischemia-reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods--a method based on ordinary differential equations (Time S...

journal_title：FEBS letters

authors： Le Pape S,Dimitrova E,Hannaert P,Konovalov A,Volmer R,Ron D,Thuillier R,Hauet T

更新日期：2014-08-25 00:00:00

abstract：:The vasopressin precursor is composed of 3 domains in line, namely vasopressin, MSEL-neurophysin and a glycopeptide referred to as copeptin, which are separated during the processing. In guinea pig neurohypophysis, the precursor is partially processed so that a two-domain fragment, MSEL-neurophysin--copeptin, can be f...

journal_title：FEBS letters

authors： Chauvet MT,Chauvet J,Acher R

更新日期：1986-03-03 00:00:00

abstract：:Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily...

journal_title：FEBS letters

authors： Kostyukova AS,Pyatibratov MG,Filimonov VV,Fedorov OV

更新日期：1988-12-05 00:00:00

abstract：:The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

journal_title：FEBS letters

authors： Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

更新日期：1985-01-01 00:00:00

abstract：:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...

journal_title：FEBS letters

authors： Law PY,Liu YM,Geng H,Kwan KH,Waye MM,Ho YY

更新日期：2006-06-26 00:00:00

abstract：:The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very...

journal_title：FEBS letters

authors： Mohr E,Bahnsen U,Kiessling C,Richter D

更新日期：1988-12-19 00:00:00

abstract：:The dynamics of chromatin structure are tightly regulated by multiple epigenetic mechanisms such as histone modifications and incorporation of histone variants. In the current work, differentiation of an embryonal carcinoma cell line, NT2, was induced by retinoic acid, and total histone proteins were compared througho...

journal_title：FEBS letters

authors： Shahhoseini M,Favaedi R,Baharvand H,Sharma V,Stunnenberg HG

更新日期：2010-11-19 00:00:00

abstract：:Muscle phosphofructokinase interacts with microtubule-associated protein-free microtubules resulting in a reduction of the overall activity of the enzyme [Lehotzky et al. (1993) J. Biol. Chem. 268, 10888-10894] and periodical cross-linking of the tubules [Lehotzky et al. (1994) Biochem. Biophys. Res. Commun. 204, 585-...

journal_title：FEBS letters

authors： Vértessy BG,Kovács J,Ovádi J

更新日期：1996-01-29 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was l...

journal_title：FEBS letters

authors： Temmerman W,Ritsema T,Simón-Mateo C,Van Montagu M,Mironov V,Inzé D,Goethals K,Holsters M

更新日期：2001-03-09 00:00:00

abstract：:Selenium-dependent glutathione peroxidase activity is documented for the first time in insects. Reduction in glutathione peroxidase activity in the cytosol of adult house flies by lowering selenium in the diet results in significant increases in peroxidative injury. Catalase activity, while higher in low-selenium flie...

journal_title：FEBS letters

authors： Simmons TW,Jamall IS,Lockshin RA

更新日期：1987-06-29 00:00:00

abstract：:Replacements of Asp-295 by Asn (D295N) and Glu (D295E) decreased the catalytic center activity of Leuconostoc mesenteroides sucrose phosphorylase to about 0.01% of the wild-type level (k(cat)=200s(-1)). Glucosylation and deglucosylation steps of D295N were affected uniformly, approximately 10(4.3)-fold, and independen...

journal_title：FEBS letters

authors： Mueller M,Nidetzky B

更新日期：2007-04-03 00:00:00

abstract：:We have studied the pharmacological properties of genetically engineered human NK1 tachykinin receptors in which residues at the extracellular surface of the fourth transmembranal domain were substituted with the corresponding amino acids from the NK2 receptor. We show that substitution of G166C:Y167F in the human NK1...

journal_title：FEBS letters

authors： Ciucci A,Palma C,Riitano D,Manzini S,Werge TM

更新日期：1997-10-27 00:00:00

abstract：:The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

journal_title：FEBS letters

authors： Yamaguchi N,Yamaguchi N

更新日期：2015-05-22 00:00:00

abstract：:The bi-functional protein dimerization cofactor of HNF1 (DCoH)/pterin-4 alpha-carbinolamine dehydratase (PCD) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 (HNF1) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system. Deficiency...

journal_title：FEBS letters

authors： Ficner R,Sauer UH,Ceska TA,Stier G,Suck D

更新日期：1995-01-02 00:00:00