Abstract:
:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of electrogenic stages, which is probably associated with Cl- translocation in the cytoplasmic direction. Cl- translocation efficiency of these purple forms is much lower than that of the proton transport by the wild-type bacteriorhodopsin. The values of the efficiency do not exceed 15, 8 and 3% for the D85T, D85S and wild-type acid purple form, respectively. Cl- induces an additional electrogenic phase in the photovoltage responses of the D85S mutant and the wild-type acid purple form. This phase is supposed to be associated with the reversible Cl- movement in the extracellular direction. It is interesting that this component is absent in the photovoltage response of the D85T mutant which has, like halorhodopsin, a threonine residue at position 85.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kalaidzidis IV,Kaulen ADdoi
10.1016/s0014-5793(97)01390-2subject
Has Abstractpub_date
1997-12-01 00:00:00pages
239-42issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01390-2journal_volume
418pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Most geckos are nocturnal forms and possess rod retinas, but some diurnal genera have pure-cone retinas. We isolated cDNAs encoding the diurnal gecko opsins, dg1 and dg2, similar to nocturnal gecko P521 and P467, respectively. Despite the large morphological differences between the diurnal and nocturnal gecko photorec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00089-7
更新日期:1999-02-19 00:00:00
abstract::On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80033-d
更新日期:1992-02-24 00:00:00
abstract::Cyclic AMP-mediated phosphorylation of calcium channel subunits was studied in vitro and in vivo in preparations from dog heart. Calcium channels in native cardiac membranes were phosphorylated by cAMP-dependent protein kinase (PKA) solubilized with digitonin and subsequently immunoprecipitated using a polyclonal anti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80733-b
更新日期:1993-12-06 00:00:00
abstract::Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80478-7
更新日期:1987-11-30 00:00:00
abstract::Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01429-0
更新日期:2000-04-21 00:00:00
abstract::Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80134-0
更新日期:1988-07-18 00:00:00
abstract::The aim of this work was to provide an overview of the genetics of human ageing to gain novel insights about the mechanisms involved. By incorporating findings from model organisms to humans, such as mutations that either delay or accelerate ageing in mice, we constructed the gene networks previously related to ageing...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.006
更新日期:2004-07-30 00:00:00
abstract::At present, little is known about signal transduction mechanisms in schistosomes, which cause the disease of schistosomiasis. The mitogen-activated protein kinase (MAPK) signaling pathways, which are evolutionarily conserved from yeast to Homo sapiens, play key roles in multiple cellular processes. Here, we reconstruc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.055
更新日期:2006-06-26 00:00:00
abstract::Tau proteins are abnormally phosphorylated in Alzheimer's disease. Pathological Tau proteins named PHF-Tau 55, PHF-Tau 64, and PHF-Tau 69, are the main constituents of the paired helical filaments (PHF). When treating SKNSH-SY 5Y cells with okadaic acid (OA), Tau 55 protein was clearly induced whereas Tau 64 protein w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01361-4
更新日期:1995-01-03 00:00:00
abstract::Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00144-6
更新日期:1998-03-06 00:00:00
abstract::The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00617-2
更新日期:1994-07-25 00:00:00
abstract::To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13124
更新日期:2018-07-01 00:00:00
abstract::The 77 kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3' end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.03.007
更新日期:2010-04-16 00:00:00
abstract::We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80867-t
更新日期:1993-12-28 00:00:00
abstract::Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.025
更新日期:2012-10-19 00:00:00
abstract::Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80822-9
更新日期:1983-11-14 00:00:00
abstract::Chronic inflammation associated with tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS) is the hallmark of tuberculosis. Mycobacterium tuberculosis (MTB) directly stimulates human monocytes to secrete TNF-alpha. We show the augmented expression of TNF-alpha mRNA in MTB-infected monocytes by cellular a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.071
更新日期:2006-05-01 00:00:00
abstract::During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80917-6
更新日期:1992-11-09 00:00:00
abstract::Analyzing the primary structure we predicted that calponin may interact with phospholipids. In order to check this suggestion we investigated the interaction of calponin with phospholipids by ultracentrifugation, light scattering, vesicles leakage and differential scanning calorimetry. In agreement with our prediction...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00868-a
更新日期:1995-09-04 00:00:00
abstract::The KDEL receptor is a seven-transmembrane-domain protein that was first described about 20 years ago. Its well-known function is to retrotransport chaperones from the Golgi complex to the endoplasmic reticulum. Recent studies, however, have suggested that the KDEL receptor has additional functions. Indeed, we have de...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.10.053
更新日期:2009-12-03 00:00:00
abstract::The carbohydrate binding properties of jacalin lectin were examined using RAF9 cell-derived D-[6-3H]glucosamine-radiolabeled total glycopeptides containing N-linked and O-linked oligosaccharides. The binding of N-linked glycopeptides to jacalin was abolished by treatment of alpha-galactosidase whereas O-linked glycope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01539-1
更新日期:1998-01-09 00:00:00
abstract::Equine platelet beta tropomyosin (247 residues), like rabbit skeletal muscle alpha tropomyosin (284 residues) has a repeating pattern of amino acid residues characteristic of a coiled-coil structure. When compared with the muscle protein, it is extended by 5 residues at the NH2-terminus and possesses two 21 residue de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80511-0
更新日期:1983-06-13 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80472-0
更新日期:1986-04-21 00:00:00
abstract::Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01663-4
更新日期:1999-12-17 00:00:00
abstract::Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.04.030
更新日期:2009-05-19 00:00:00
abstract::The known functions of type II thioesterases (TEIIs) in type I polyketide synthases (PKSs) include selecting of starter acyl units, removal of aberrant extender acyl units, releasing of final products, and dehydration of polyketide intermediates. In this study, we characterized two TEIIs (ScnI and PKSIaTEII) from Stre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.07.010
更新日期:2014-08-25 00:00:00
abstract::Antibodies were made to a thirteen amino acid synthetic peptide corresponding to the C-terminal portion of the glutamate (glu) receptor, GluR-A. The immunoprecipitation of kainic acid (KA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) binding sites by the anti-peptide antibodies was studied using...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80529-r
更新日期:1990-12-10 00:00:00
abstract::Tom1 (Target of Myb1) is suggested to be involved in the transport of ubiquitinated proteins, through the interaction of its GAT (GGA and Tom1) domain with ubiquitin. Here, we demonstrate that the three-helix bundle of Tom1-GAT has two ubiquitin-binding sites recognizing the hydrophobic Ile44 surface of ubiquitin. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.076
更新日期:2005-10-10 00:00:00
abstract::Amyloid-beta peptide (Abeta), the major constituent of senile plaques in the Alzheimer's disease (AD) brain, is the main source of oxidative stress leading to neurodegeneration. The methionine residue in this peptide is reported to be responsible for neurotoxicity. Structurally similar substitution with methionine 35 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.038
更新日期:2007-04-03 00:00:00