Abstract:
:Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecting the channel characteristics. (ii) Substitution of the first glycine (G77) residue within the GYG motif by an alanine or substitution of the tyrosine (Y) residue 78 by a phenylalanine (F) led to mutant proteins which form tetramers of reduced stability. In contrast to the AYG mutant protein, GFG functions as an active K(+) channel whose characteristics correspond to those of the wild-type KcsA channel. (iii) The investigated mutant proteins, which carry different mutations (T72A, T72C, V76A, V76E, G77E, Y78A, G79A, G79D, G79E) within the signature sequence of the pore region, do not at all or only to a very small degree assemble as tetramers and lack channel activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Splitt H,Meuser D,Borovok I,Betzler M,Schrempf Hdoi
10.1016/s0014-5793(00)01429-0keywords:
subject
Has Abstractpub_date
2000-04-21 00:00:00pages
83-7issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01429-0journal_volume
472pub_type
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