Characterization of type II thioesterases involved in natamycin biosynthesis in Streptomyces chattanoogensis L10.

abstract：:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

journal_title：FEBS letters

authors： Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

更新日期：1986-09-01 00:00:00

abstract：:We have recently cloned a cDNA encoding sucrose:fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the beta-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652-11656]. Here we report functional expression of 6-SFT...

journal_title：FEBS letters

authors： Sprenger N,Schellenbaum L,van Dun K,Boller T,Wiemken A

更新日期：1997-01-06 00:00:00

abstract：:Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...

journal_title：FEBS letters

authors： Schwabe A,Maarleveld TR,Bruggeman FJ

更新日期：2013-09-02 00:00:00

abstract：:We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...

journal_title：FEBS letters

authors： Stolz A,Sugiyama Y,Kuhlenkamp J,Kaplowitz N

更新日期：1984-11-05 00:00:00

abstract：:A novel rapid polymerase chain reaction (PCR)-based technique for N-terminal attachment of green fluorescent protein (GFP) to a yeast protein is described. Genomic integration of a PCR-generated loxPkanMX4loxP-yEGFP fusion cassette immediately upstream of the open reading frame (ORF) allows for selection of G418 resis...

journal_title：FEBS letters

authors： Prein B,Natter K,Kohlwein SD

更新日期：2000-11-17 00:00:00

abstract：:This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This...

journal_title：FEBS letters

authors： Senapathy P,Ali MA,Jacob MT

更新日期：1985-10-14 00:00:00

abstract：:Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...

journal_title：FEBS letters

authors： Li M,Zhou W,Yuan R,Chen L,Liu T,Huang D,Hao L,Xie Y,Shao J

更新日期：2015-04-13 00:00:00

abstract：:A variety of plant tissues contain an uncoupling mitochondrial protein (PUMP), recently described and characterized by our group. In this study we show that the inhibition of PUMP activity in potato tuber mitochondria significantly increases mitochondrial H2O2 generation, while PUMP substrates, such as linoleic acid, ...

journal_title：FEBS letters

authors： Kowaltowski AJ,Costa AD,Vercesi AE

更新日期：1998-03-27 00:00:00

abstract：:The trk family of tyrosine protein kinase genes serves crucial roles for the development of the nervous system and the survival of neurons. The members of this gene family, trk, trkB and trkC, bind a distinct neurotrophin of the nerve growth factor (NGF) gene family, and trigger the intracellular signals which elicit ...

journal_title：FEBS letters

authors： Okazawa H,Kamei M,Kanazawa I

更新日期：1993-08-23 00:00:00

abstract：:A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...

journal_title：FEBS letters

authors： Mitchell DM,Gennis RB

更新日期：1995-07-10 00:00:00

abstract：:Cathepsin L is a ubiquitously expressed papain-like cysteine protease involved in the endosomal degradation of proteins and has numerous roles in physiological and pathological processes, such as arthritis, osteoporosis, and cancer. Insight into the specificity of cathepsin L is important for elucidating its physiolog...

journal_title：FEBS letters

authors： Sosnowski P,Turk D

更新日期：2016-04-01 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit the effects of proinflammatory cytokines such as interleukin-1beta (IL-1beta). This cytokine plays a key role in articular pathophysiologies by inducing the production of inflammatory mediators such as nitric oxide (NO) and...

journal_title：FEBS letters

authors： Boyault S,Bianchi A,Moulin D,Morin S,Francois M,Netter P,Terlain B,Bordji K

更新日期：2004-08-13 00:00:00

abstract：:We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-b...

journal_title：FEBS letters

authors： Kase R,Kitagawa H,Hayashi K,Tanoue K,Inagaki F

更新日期：1989-08-28 00:00:00

abstract：:Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...

journal_title：FEBS letters

authors： Olins GM,Mehta PP,Blehm DJ,Patton DR,Zupec ME,Whipple DE,Tjoeng FS,Adams SP,Olins PO,Gierse JK

更新日期：1987-11-30 00:00:00

abstract：:Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-L-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted ...

journal_title：FEBS letters

authors： Sengupta R,Billiar TR,Atkins JL,Kagan VE,Stoyanovsky DA

更新日期：2009-11-03 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...

journal_title：FEBS letters

authors： Stavri GT,Hong Y,Zachary IC,Breier G,Baskerville PA,Ylä-Herttuala S,Risau W,Martin JF,Erusalimsky JD

更新日期：1995-01-30 00:00:00

abstract：:A simple model is put forward to explain the long-known three-base periodicity in coding DNA. We propose the concept of same-phase triplet clustering, i.e. a condition wherein a triplet appears several times in one phase without interruption by the two other possible phases. For instance, in the sequence (i): NTT_GNN_...

journal_title：FEBS letters

authors： Sánchez J,López-Villaseñor I

更新日期：2006-11-27 00:00:00

abstract：:A major house dust mite allergen Der f 1 belongs to the papain-like cysteine protease family. This study investigated whether Der f 1 can cleave the latency-associated peptide (LAP) of transforming growth factor (TGF)-beta via its proteolytic activity and activate latent TGF-beta. We found that Der f 1 can cleave LAP ...

journal_title：FEBS letters

authors： Nakamura Y,Miyata M,Shimokawa N,Ohnuma Y,Katoh R,Ogawa H,Okumura K,Nakao A

更新日期：2009-06-18 00:00:00

abstract：:We studied the effects of fibroblast growth factor (FGF-10) on alveolar epithelial cell (AEC) Na,K-ATPase regulation. Within 30 min FGF-10 increased Na,K-ATPase activity and alpha1 protein abundance by 2.5-fold at the AEC plasma membrane. Pretreatment of AEC with the mitogen-activated protein kinase (MAPK) inhibitor U...

journal_title：FEBS letters

authors： Upadhyay D,Lecuona E,Comellas A,Kamp DW,Sznajder JI

更新日期：2003-06-19 00:00:00

abstract：:The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptak...

journal_title：FEBS letters

authors： Sagi-Eisenberg R,Geller-Bernstein C,Ben-Neriah Z,Pecht I

更新日期：1983-09-05 00:00:00

abstract：:A UV irradiation-induced mutant of Bacillus sphaericus 2362 whose sporulation was inhibited neither by natural amino acids nor by L-ethionine was selected. The mutant (A61) grew slowly in rich amino acid medium and contained increased concentrations of heat-resistant spores throughout the growth. Slow growth of A61 wa...

journal_title：FEBS letters

authors： Andreev J,Dibrov PA,Braun S

更新日期：1994-06-27 00:00:00

abstract：:Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of mos...

journal_title：FEBS letters

authors： Gamcsik MP,Gerig JT

更新日期：1986-02-03 00:00:00

abstract：:We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...

journal_title：FEBS letters

authors： Epand RM,Stafford A,Wang J,Epand RF

更新日期：1992-06-15 00:00:00

abstract：:ATM (ataxia telangiectasia mutated), the gene mutated in ataxia telangiectasia, is related to a family of large phosphatidylinositol 3-kinase domain-containing proteins involved in cell cycle control and DNA repair. We found that ATM(-/-) DT40 cells were more susceptible than wild-type cells to apoptosis induced not o...

journal_title：FEBS letters

authors： Takao N,Li Y,Yamamoto K

更新日期：2000-04-21 00:00:00

abstract：:The ARG-11 gene in Saccharomyces cerevisiae encodes a protein with the characteristic features of a family of 35 related membrane proteins that are encoded in the fungal genome. Some of them are known to transport various substrates and products across the inner membranes of mitochondria, but the functions of 29 membe...

journal_title：FEBS letters

authors： Palmieri L,De Marco V,Iacobazzi V,Palmieri F,Runswick MJ,Walker JE

更新日期：1997-06-30 00:00:00

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:The study of the three-dimensional organization of chromatin has recently gained much focus in the context of novel techniques for detecting genome-wide contacts using next-generation sequencing. These chromosome conformation capture-based methods give a deep topological insight into the architecture of the genome ins...

journal_title：FEBS letters

authors： Hofmann A,Heermann DW

更新日期：2015-10-07 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

journal_title：FEBS letters

authors： Mayer B,Schmid M,Klatt P,Schmidt K

更新日期：1993-10-25 00:00:00

abstract：:The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in t...

journal_title：FEBS letters

authors： Hirose T,Kusumegi T,Sugiura M

更新日期：1998-07-03 00:00:00

abstract：:The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...

journal_title：FEBS letters

authors： Ouzounis C,Kyrpides N

更新日期：1996-07-22 00:00:00