Neutralizing monoclonal antibody specific for alpha-bungarotoxin: preparation and characterization of the antibody, and localization of antigenic region of alpha-bungarotoxin.

abstract：:The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ter...

journal_title：FEBS letters

authors： Mercaldi GF,Dawson A,Hunter WN,Cordeiro AT

更新日期：2016-08-01 00:00:00

abstract：:Globular protein thermostability is characterized the cold denaturation, maximal stability (Tms) and heat denaturation temperatures. For mesophilic globular proteins, Tms typically ranges from -25 degrees C to +35 degrees C. We show that the indirect estimate of Tms from calorimetry and the direct estimate from chemic...

journal_title：FEBS letters

authors： Ganesh C,Eswar N,Srivastava S,Ramakrishnan C,Varadarajan R

更新日期：1999-07-02 00:00:00

abstract：:The metabolism of 20:4 (arachidonic acid) in alkenylacyl, alkylacyl and diacyl lipid classes in choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP) in rabbit alveolar macrophages was examined. [3H]20:4 was very rapidly incorporated into diacyl glycerophosphocholine (GPC). After the removal o...

journal_title：FEBS letters

authors： Sugiura T,Katayama O,Fukui J,Nakagawa Y,Waku K

更新日期：1984-01-09 00:00:00

abstract：:A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...

journal_title：FEBS letters

authors： Herrmann JL,Delahay R,Gallagher A,Robertson B,Young D

更新日期：2000-05-19 00:00:00

abstract：:Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...

journal_title：FEBS letters

authors： Bersani M,Johnsen AH,Holst JJ

更新日期：1991-02-25 00:00:00

abstract：:Chronic indirect stimulation of fast twitch rabbit muscle induced a rapid reduction in parvalbumin concentration. When compared to the unstimulated contralateral muscle, parvalbumins were reduced to 55% following 6 days of stimulation. Prolonged stimulation further reduced parvalbumins so that they were undetectable a...

journal_title：FEBS letters

authors： Klug G,Reichmann H,Pette D

更新日期：1983-02-21 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

journal_title：FEBS letters

authors： Li LQ,Pan D,Chen H,Zhang L,Xie WJ

更新日期：2016-02-01 00:00:00

abstract：:We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a...

journal_title：FEBS letters

authors： Hwang MG,Chung IK,Kang BG,Cho MH

更新日期：2001-08-10 00:00:00

abstract：:Under heat shock conditions translation of Xenopus laevis normal mRNAs in a rabbit reticulocyte cell-free system is blocked whereas hsp70 mRNA is translated. mRNA for E. coli beta-galactosidase containing the last four sense codons of Drosophila hsp70 at its 3'-end was constructed. This mRNA is efficiently translated ...

journal_title：FEBS letters

authors： Denisenko ON,Yarchuk OB

更新日期：1989-04-24 00:00:00

abstract：:A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...

journal_title：FEBS letters

authors： Janecek S

更新日期：1995-12-11 00:00:00

abstract：:Here, we investigated the molecular mechanism underlying the changes in the distribution of nucleolin. Our study identified PI3K/Akt signaling as an essential pathway regulating the distribution of nucleolin. Furthermore, nucleolin can interact with phospho-PI3K-p55, and changes in the distribution of nucleolin were r...

journal_title：FEBS letters

authors： Wu DM,Zhang P,Liu RY,Sang YX,Zhou C,Xu GC,Yang JL,Tong AP,Wang CT

更新日期：2014-05-21 00:00:00

abstract：:The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...

journal_title：FEBS letters

authors： Kotelnikova RA,Kotelnikov AI,Bogdanov GN,Romanova VS,Kuleshova EF,Parnes ZN,Vol'pin ME

更新日期：1996-07-01 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:Retention of intron 8 in alternative HER-2 mRNA generates an inhibitory secreted ligand, Herstatin, with a novel receptor-binding domain (RBD) encoded by the intron. This study examines binding interactions with several receptors and investigates sequence variations in intron 8. The RBD, expressed as a peptide, binds ...

journal_title：FEBS letters

authors： Shamieh LS,Evans AJ,Denton MC,Clinton GM

更新日期：2004-06-18 00:00:00

abstract：:A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...

journal_title：FEBS letters

authors： Mayer R,Lancelot G,Hélène C

更新日期：1983-03-21 00:00:00

abstract：:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...

journal_title：FEBS letters

authors： Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt W

更新日期：2015-07-08 00:00:00

abstract：:The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both puri...

journal_title：FEBS letters

authors： Biral D,Volpe P,Damiani E,Margreth A

更新日期：1992-03-09 00:00:00

abstract：:The yeast Saccharomyces cerevisiae contains two acetyl-CoA synthetase genes, ACS1 and ACS2. While ACS1 transcription is glucose repressible, ACS2 shows coregulation with structural genes of fatty acid biosynthesis. The ACS2 upstream region contains an ICRE (inositol/choline-responsive element) as an activating sequenc...

journal_title：FEBS letters

authors： Hiesinger M,Wagner C,Schüller HJ

更新日期：1997-09-22 00:00:00

abstract：:Biological systems, from macromolecules to whole organisms, are robust if they continue to function, survive, or reproduce when faced with mutations, environmental change, and internal noise. I focus here on biological systems that are robust to mutations and ask whether such systems are more or less evolvable, in the...

journal_title：FEBS letters

authors： Wagner A

更新日期：2005-03-21 00:00:00

abstract：:The fungal metabolite brefeldin A (BFA) is known to disrupt the Golgi apparatus resulting in redistribution of Golgi proteins to the endoplasmic reticulum and inhibition of protein secretion. BFA was found to inhibit protein synthesis in rat glioma C6 cells by up to 70% between 0.1 and 1 microgram/ml. Inhibition was b...

journal_title：FEBS letters

authors： Fishman PH,Curran PK

更新日期：1992-12-21 00:00:00

abstract：:Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

journal_title：FEBS letters

authors： Wu X,Zhou Y,Zhang K,Liu Q,Guo D

更新日期：2008-06-25 00:00:00

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:The gene trap methodology is a powerful tool to characterize novel genes and analyze their importance in biological phenomena. It is based on the use of mouse embryonic stem cells and reporter vectors designed to randomly integrate into the genome, tagging an insertion site and generating a mutation. Theoretically, al...

journal_title：FEBS letters

authors： Cecconi F,Meyer BI

更新日期：2000-08-25 00:00:00

abstract：:The potent inhibitors of protein kinase C (PKC), H7, staurosporine, and staurosporine derivatives, were examined for their inhibitory effects on novel PKC (nPKC) isozymes delta and epsilon. H7 and staurosporine, usually used as selective inhibitors of PKC, showed similar inhibitory effects on cPKC (a mixture of cPKC a...

journal_title：FEBS letters

authors： Mizuno K,Saido TC,Ohno S,Tamaoki T,Suzuki K

更新日期：1993-09-13 00:00:00

abstract：:A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group....

journal_title：FEBS letters

authors： Cox MC,Rogers MS,Cheesman M,Jones GD,Thomson AJ,Wilson MT,Moore GR

更新日期：1992-07-28 00:00:00

abstract：:Regulation of acetylcholine receptor (AChR) gene expression was analyzed in alpha-bungarotoxin (alpha-BTX) treated rats. A reduction in available 125I-alpha-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR alpha-, beta- epsilon- and delta-subunit mRNAs was simi...

journal_title：FEBS letters

authors： Asher O,Provenzano C,Fuchs S

更新日期：1991-05-06 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:Dosage compensation involves fine-tuning of gene expression at the level of entire chromosomes. The principles that assure selective targeting of the male X chromosome in Drosophila and the mechanism by which transcription levels are adjusted in a twofold range are still mysterious. We discuss the prevalent models in ...

journal_title：FEBS letters

authors： Straub T,Dahlsveen IK,Becker PB

更新日期：2005-06-13 00:00:00

abstract：:In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...

journal_title：FEBS letters

authors： Pedraza CE,Baltrons MA,García A

更新日期：2001-11-02 00:00:00