Close evolutionary relatedness among functionally distantly related members of the (alpha/beta)8-barrel glycosyl hydrolases suggested by the similarity of their fifth conserved sequence region.

abstract：:Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...

journal_title：FEBS letters

authors： Gebhardt R,Ebert A,Bauer G

更新日期：1988-12-05 00:00:00

abstract：:Signal transduction involving phosphatidylcholine hydrolysis has been investigated in human neutrophils (PMN) after in situ generation of [3H]alkylacyl-sn-glycero-3-phosphocholine ([3H]alkylacyl-GPC) by cell incubation with [3H]alkylacetyl-GPC. When PMN were stimulated with the chemotactic peptide N-formyl-Met-Leu-Phe...

journal_title：FEBS letters

authors： Gelas P,Ribbes G,Record M,Terce F,Chap H

更新日期：1989-07-17 00:00:00

abstract：:Recombinant expression of the large alternansucrase (2057 amino acids) was hindered in E. coli due to poor enzyme solubility and protein degradation. The effects of deletions of the alternansucrase C-terminal CW-like and APY repeated motifs on enzyme solubility and specificity were investigated. A truncated variant de...

journal_title：FEBS letters

authors： Joucla G,Pizzut S,Monsan P,Remaud-Simeon M

更新日期：2006-02-06 00:00:00

abstract：:We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly re...

journal_title：FEBS letters

authors： Chessell IP,Simon J,Hibell AD,Michel AD,Barnard EA,Humphrey PP

更新日期：1998-11-13 00:00:00

abstract：:In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...

journal_title：FEBS letters

authors： Kamachi F,Yanai M,Ban HS,Ishihara K,Hong J,Ohuchi K,Hirasawa N

更新日期：2007-10-02 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00

abstract：:The human DNA-dependent activator of IFN-regulatory factor (DAI) protein, which activates the innate immune response in response to DNA, contains two tandem Z-DNA binding domains (Zα and Zβ) at the NH(2) terminus. The hZβ(DAI) structure is similar to other Z-DNA binding proteins, although it demonstrates an unusual Z-...

journal_title：FEBS letters

authors： Kim HE,Ahn HC,Lee YM,Lee EH,Seo YJ,Kim YG,Kim KK,Choi BS,Lee JH

更新日期：2011-03-09 00:00:00

abstract：:Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...

journal_title：FEBS letters

authors： King IA,Magee AI,Rees DA,Buxton RS

更新日期：1991-07-29 00:00:00

abstract：:We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-alpha)- and receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly in...

journal_title：FEBS letters

authors： Yamaguchi N,Kukita T,Li YJ,Kamio N,Fukumoto S,Nonaka K,Ninomiya Y,Hanazawa S,Yamashita Y

更新日期：2008-02-06 00:00:00

abstract：:Metabolic control theory is extended to include channelled metabolism in general. A simple relationship between the flux control by the enzymes and the degree of metabolite channelling is derived. This relationship suggests experiments in which modulation of gene expression allows one to quantify channelling. ...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV

更新日期：1993-03-29 00:00:00

abstract：:In higher plants, two types of nitrate transporters, NRT1 and NRT2, have been identified. In Arabidopsis, there are 53 NRT1 genes and 7 NRT2 genes. NRT2 are high-affinity nitrate transporters, while most members of the NRT1 family are low-affinity nitrate transporters. The exception is CHL1 (AtNRT1.1), which is a dual...

journal_title：FEBS letters

authors： Tsay YF,Chiu CC,Tsai CB,Ho CH,Hsu PK

更新日期：2007-05-25 00:00:00

abstract：:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and sp...

journal_title：FEBS letters

authors： Durchschlag H,Zipper P

更新日期：1988-09-12 00:00:00

abstract：:Human uncoupling protein 3 (UCP3) has two RNA transcripts that arise from the differential processing of the same gene product. One encodes the full length protein (UCP3L) while the other encodes a truncated version (UCP3S) lacking the sixth membrane spanning domain. The roles of the two isoforms are not known, but a ...

journal_title：FEBS letters

authors： Renold A,Koehler CM,Murphy MP

更新日期：2000-01-14 00:00:00

abstract：:It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

journal_title：FEBS letters

authors： Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

更新日期：1991-06-03 00:00:00

abstract：:Interleukin-4 (IL-4) is a pleiotropic cytokine, which acts on both hematopoietic and non-hematopoietic cells, through different types of receptor complexes. In this study, we report that in human B cells, IL-4 caused rapid phosphorylation of Janus kinase (JAK) 1 and JAK3 tyrosine kinases. In keratinocytes, the hematop...

journal_title：FEBS letters

authors： Wery-Zennaro S,Letourneur M,David M,Bertoglio J,Pierre J

更新日期：1999-12-24 00:00:00

abstract：:Saccharomyces cerevisiae Vig4/Vrg4 protein is a Golgi membrane protein which has multiple transmembrane domains and is essential for transport of GDP-mannose across the Golgi membrane. By immunoprecipitation of detergent-solubilized tagged protein, we found that this protein exists as oligomer. Two mutants vig4-1 and ...

journal_title：FEBS letters

authors： Abe M,Hashimoto H,Yoda K

更新日期：1999-09-24 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:Golgin45 plays important roles in Golgi stack assembly and is known to bind both the Golgi stacking protein GRASP55 and Rab2 in the medial-Golgi cisternae. In this study, we sought to further characterize the cisternal adhesion complex using a proteomics approach. We report here that Acyl-CoA binding domain containing...

journal_title：FEBS letters

authors： Yue X,Bao M,Christiano R,Li S,Mei J,Zhu L,Mao F,Yue Q,Zhang P,Jing S,Rothman JE,Qian Y,Lee I

更新日期：2017-09-01 00:00:00

abstract：:Choroidal melanoma (CM) remains the most prevalent form of intraocular malignancy, and the prognosis of affected patients is poor. While the E3 ubiquitin ligase TRAF-interacting protein (TRIP) is known to play key regulatory roles in multiple diseases, its relevance in CM remains uncertain. In the present study, we fo...

journal_title：FEBS letters

authors： Wei C,Zhao X,Wang L,Zhang H

更新日期：2020-10-01 00:00:00

abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

journal_title：FEBS letters

authors： Shannon MF,Wigley PL,Wells JR

更新日期：1985-07-08 00:00:00

abstract：:Rabphilin is a synaptic vesicle-associated protein proposed to play a role in regulating neurotransmitter release. Here we report the isolation and identification of a novel protein complex containing rabphilin, annexin A4 and synaptotagmin 1. We show that the rabphilin C2B domain interacts directly with the N-terminu...

journal_title：FEBS letters

authors： Willshaw A,Grant K,Yan J,Rockliffe N,Ambavarapu S,Burdyga G,Varro A,Fukuoka S,Gawler D

更新日期：2004-02-13 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 hum...

journal_title：FEBS letters

authors： Ahmed S,Budai B,Herédi-Szabó K,Farkas J,Tóth G,Murphy RF,Lovas S

更新日期：2004-01-02 00:00:00

abstract：:Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...

journal_title：FEBS letters

authors： Guan SY,Chng CP,Ong LT,Tan HF,Alex Law SK,Tan SM

更新日期：2018-01-01 00:00:00

abstract：:Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...

journal_title：FEBS letters

authors： Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl R

更新日期：1999-10-29 00:00:00

abstract：:Respiration in all cells depends upon synthesis of ATP by the ATP synthase complex, a rotary motor enzyme. The structure of the catalytic moiety of ATP synthase, the so-called F(1) headpiece, is well established. F(1) is connected to the membrane-bound and ion translocating F(0) subcomplex by a central stalk. A periph...

journal_title：FEBS letters

authors： Dudkina NV,Heinemeyer J,Keegstra W,Boekema EJ,Braun HP

更新日期：2005-10-24 00:00:00

abstract：:The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

journal_title：FEBS letters

authors： Sitaramam V

更新日期：1989-04-10 00:00:00

abstract：:The effects of intracellular purine nucleotides on tolbutamide-induced block of ATP-dependent K+ channels from mouse pancreatic B-cells were studied using the patch-clamp technique. When applied to the inside of excised patches, tolbutamide alone blocked channel activity half-maximally at 55 microM and the concentrati...

journal_title：FEBS letters

authors： Zünkler BJ,Lins S,Ohno-Shosaku T,Trube G,Panten U

更新日期：1988-11-07 00:00:00

abstract：:Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro se...

journal_title：FEBS letters

authors： Oberholzer T,Mantei N,Semenza G

更新日期：1993-10-25 00:00:00

abstract：:Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...

journal_title：FEBS letters

authors： Moore GA,McConkey DJ,Kass GE,O'Brien PJ,Orrenius S

更新日期：1987-11-30 00:00:00