Abstract:
:Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 human colon cancer cell line. The goal of the current study was to further investigate the role of this receptor in mediating the growth-promoting effects of gastrin peptides. Binding of [Leu(15)]G17 and [Leu(15)]G17-Gly to DLD-1 cell membranes in competition with [(3)H]G17-Gly was examined. Binding of [(3)H]cholecystokinin-8 (CCK8) to DLD-1 cell membranes was also assessed. Whole cell binding experiments were carried out using [(125)I-Tyr(12),Leu(15)]G17-Gly. In addition, the ability of [Leu(15)]G17 and [Leu(15)]G17-Gly to stimulate cell growth, as determined by cell counting, was tested. [Leu(15)]G17 and [Leu(15)]G17-Gly competed with [(3)H]G17-Gly at both a high and a low affinity site on DLD-1 membranes. The IC(50) values for [Leu(15)]G17 were 6.0 x 10(-8) M and 6.9 x 10(-6) M while those for [Leu(15)]G17-Gly were 3.2 x 10(-9) M and 4.9 x 10(-6) M. [(3)H]CCK8 did not bind to either site. [Leu(15)]G17-Gly also competed with [(125)I-Tyr(12),Leu(15)]G17-Gly at both a high and a low affinity site on DLD-1 cells with similar affinities as observed with membranes. [Leu(15)]G17 and [Leu(15)]G17-Gly significantly stimulated the growth of DLD-1 cells in a dose-dependent and biphasic manner. The binding profiles of the peptides tested suggest that these sites are different from previously identified wild-type and mutant CCK(1) or CCK(2) receptors.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ahmed S,Budai B,Herédi-Szabó K,Farkas J,Tóth G,Murphy RF,Lovas Sdoi
10.1016/s0014-5793(03)01408-xkeywords:
subject
Has Abstractpub_date
2004-01-02 00:00:00pages
199-203issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930301408Xjournal_volume
556pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::1,2-Didecanoylglycerol (diC10) is taken up by human platelets and sequentially converted to 1,2-didecanoylphosphatidic acid (PA10) and 1,2-didecanoylphosphatidylinositol (PI10). Agents that increase cyclic AMP in platelets, such as prostacyclin and forskolin, sequentially convert diC10 to PA10 and PI10. They decrease ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80141-7
更新日期:1986-01-20 00:00:00
abstract::C2cd4c, encoded by a gene belonging to the C2cd4 family, contains a C2 domain conserved across species and is localized to the cytoplasm. To examine the role of C2cd4c in the pancreas, we studied its localization and generated C2cd4c knockout (KO) mice. C2cd4c was expressed in pancreatic endocrine progenitors at early...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12271
更新日期:2016-08-01 00:00:00
abstract::The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00257-a
更新日期:1995-04-03 00:00:00
abstract::The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13683
更新日期:2020-03-01 00:00:00
abstract::Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01051-5
更新日期:1998-09-18 00:00:00
abstract::Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K+); in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81058-v
更新日期:1990-10-29 00:00:00
abstract::The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81052-2
更新日期:1984-07-23 00:00:00
abstract::FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02915-5
更新日期:2001-10-19 00:00:00
abstract::Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00058-6
更新日期:1997-02-24 00:00:00
abstract::A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00856-1
更新日期:2003-09-25 00:00:00
abstract::Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.034
更新日期:2006-09-18 00:00:00
abstract::Resistance to antimalarial drugs has often threatened malaria elimination efforts and historically has led to the short-term resurgence of malaria incidences and deaths. With concentrated malaria eradication efforts currently underway, monitoring drug resistance in clinical settings complemented by in vitro drug susce...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.04.042
更新日期:2011-06-06 00:00:00
abstract::To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.090
更新日期:2004-09-24 00:00:00
abstract::A highly specific and sensitive radioimmunoassay has been established for measurement of human endothelin (hET) in human plasma. After extraction of plasma with an octyl-silica column, this assay allowed for detection of immunoreactive (IR) hET as low as 0.2 fmol/ml. In 16 healthy subjects, the mean concentration of p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80213-3
更新日期:1989-03-13 00:00:00
abstract::Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00403-1
更新日期:1997-05-12 00:00:00
abstract::Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.029
更新日期:2014-03-03 00:00:00
abstract::Equine platelet beta tropomyosin (247 residues), like rabbit skeletal muscle alpha tropomyosin (284 residues) has a repeating pattern of amino acid residues characteristic of a coiled-coil structure. When compared with the muscle protein, it is extended by 5 residues at the NH2-terminus and possesses two 21 residue de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80511-0
更新日期:1983-06-13 00:00:00
abstract::Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have deve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01250-3
更新日期:2000-03-03 00:00:00
abstract::The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.05.024
更新日期:2012-07-30 00:00:00
abstract::Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00177-4
更新日期:1997-03-24 00:00:00
abstract::The adult liver-specific IGF-II promoter P1 is activated by CCAAT/Enhancer Binding Proteins alpha and beta. Here we present evidence that promoter P1, in addition to positively regulating elements, contains two elements of 67 nucleotides that form an inverted repeat (IR) and suppress P1 activity. The two IR elements a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00926-x
更新日期:1996-09-23 00:00:00
abstract::Stimulated neutrophils exhibit coordinated sinusoidal oscillations in filamentous actin content and cellular shape. We investigated the effect of inhibition of the small G protein Rho on neutrophil actin polymerization, shape changes and oscillations using a genetically engineered toxin that enters cells and selective...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00880-i
更新日期:1995-09-25 00:00:00
abstract::Photoaffinity labelling with [alpha-32P]GTP allowed to detect a 54 kDa GTP-binding protein in rat pancreatic plasma membranes and in pancreatic AR 4-2J cell membranes. Like the 42 and 48 kDa Gs alpha subunits and the 41 kDa Gi alpha subunit, this protein was absent from zymogen granule membranes. Contrastingly, a new ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80362-m
更新日期:1990-10-01 00:00:00
abstract::We have used the polymerase chain reaction (PCR) to synthesise a cDNA encoding part of human cardiac troponin I. Amplification was achieved using fully degenerate sets of oligonucleotides corresponding to conserved regions of amino acid sequence identified in other troponin I isoforms. The cloned PCR fragment was subs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81234-f
更新日期:1990-09-17 00:00:00
abstract::The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an alpha- and a beta-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80958-w
更新日期:1993-08-09 00:00:00
abstract::To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02994-0
更新日期:2002-07-31 00:00:00
abstract::ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.03.040
更新日期:2012-05-07 00:00:00
abstract::We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00388-2
更新日期:1994-05-16 00:00:00
abstract::A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02253-5
更新日期:2000-12-08 00:00:00
abstract::1H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment 1 suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could poss...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80063-m
更新日期:1992-02-24 00:00:00