Abstract:
:Rabphilin is a synaptic vesicle-associated protein proposed to play a role in regulating neurotransmitter release. Here we report the isolation and identification of a novel protein complex containing rabphilin, annexin A4 and synaptotagmin 1. We show that the rabphilin C2B domain interacts directly with the N-terminus of annexin A4 and mediates the co-complexing of these two proteins in PC12 cells. Analyzing the cellular localisation of these co-complexing proteins we find that annexin A4 is located on synaptic membranes and co-localises with rabphilin at the plasma membrane in PC12 cells. Given that rabphilin and synaptotagmin are synaptic vesicle proteins involved in neurotransmitter release, the identification of this complex suggests that annexin A4 may play a role in synaptic exocytosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Willshaw A,Grant K,Yan J,Rockliffe N,Ambavarapu S,Burdyga G,Varro A,Fukuoka S,Gawler Ddoi
10.1016/S0014-5793(03)01513-8keywords:
subject
Has Abstractpub_date
2004-02-13 00:00:00pages
13-21issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303015138journal_volume
559pub_type
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