Search for a dextransucrase minimal motif involved in dextran binding.

Abstract:

:Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino acids N1397 and A1527 of the C-terminal domain (GBD-7) and consists of six YG repeats. With a dissociation constant K(d) of 2.8x10(-9)M, this motif shows a very high affinity for isomaltohexaose and longer dextrans, supporting the proposed role of GBD in polymer formation. The potential application of GBD-7 as an affinity tag onto cheap resins like Sephacryl S300HR for rapid purification was evaluated and is discussed.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Suwannarangsee S,Moulis C,Potocki-Veronese G,Monsan P,Remaud-Simeon M,Chulalaksananukul W

doi

10.1016/j.febslet.2007.08.062

subject

Has Abstract

pub_date

2007-10-02 00:00:00

pages

4675-80

issue

24

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(07)00944-1

journal_volume

581

pub_type

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