Abstract:
:The role of low M(r) phosphotyrosine protein phosphatase (PTPase) in the control of cell proliferation was studied. A synthetic gene coding for PTPase was transfected and expressed in NIH/3T3 fibroblasts. The effects of the enzyme were particularly evident when cells were stimulated by platelet-derived growth factor (PDGF). The mitogenic response to PDGF was decreased and the inhibition reached 90%. This effect was more pronounced with respect to fetal calf serum stimulation. Hormone-dependent autophosphorylation of the PDGF receptor was significantly reduced. These results demonstrate that low M(r) PTPase, a cytosolic enzyme, not only affects cellular response to PDGF but also reduces the membrane receptor autophosphorylation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Berti A,Rigacci S,Raugei G,Degl'Innocenti D,Ramponi Gdoi
10.1016/0014-5793(94)00620-2subject
Has Abstractpub_date
1994-07-25 00:00:00pages
7-12issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00620-2journal_volume
349pub_type
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