Abstract:
:The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Denoel PA,Zygmunt MS,Weynants V,Tibor A,Lichtfouse B,Briffeuil P,Limet JN,Letesson JJdoi
10.1016/0014-5793(95)00189-gsubject
Has Abstractpub_date
1995-03-20 00:00:00pages
238-42issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00189-Gjournal_volume
361pub_type
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