Abstract:
:The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex lacking stroma side subunits was also present. PSI subunits in the dgd1 mutant were decreased to a similar level compared with that in the wild type (WT) Arabidopsis. Further experiments showed that PSI subunits in the stroma side, PsaD and PsaE, in the dgd1 mutant were more susceptible to removal by chaotropic agents than those in the WT plant, indicating that the stability of PsaD and PsaE is impaired in the dgd1 mutant. These results provide evidence that DGDG is important for the stability of the PSI complex.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Guo J,Zhang Z,Bi Y,Yang W,Xu Y,Zhang Ldoi
10.1016/j.febslet.2005.05.049keywords:
subject
Has Abstractpub_date
2005-07-04 00:00:00pages
3619-24issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00657-5journal_volume
579pub_type
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