Abstract:
:Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations of light and heavy lysosomes, fairly homogenous, carried sialidase activity, assayed on MU-NeuAc. The optimal pH was 4.0 and 4.2, the apparent Km value 2.8 x 10(-5) M and 4.2 x 10(-5) M and the apparent Vmax value 0.11 and 0.47 mU.mg-1 protein, for the light and heavy lysosome sialidase, respectively. From 4 days to adulthood the specific activity of the light and heavy lysosome sialidase increased 3-fold and 1.7-fold, respectively.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fiorilli A,Siniscalco C,Chiarini A,Di Francesco L,Venerando B,Tettamanti Gdoi
10.1016/0014-5793(91)80485-lsubject
Has Abstractpub_date
1991-05-06 00:00:00pages
235-8issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(91)80485-Ljournal_volume
282pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We present evidence that gastrin, binding to a G protein-coupled receptor, activates the p38-mitogen-activated protein kinase (MAPK) pathway. Blockage of protein kinase C (PKC) by GF109203X, depletion of intracellular calcium by thapsigargin or inhibition of Src family kinases by PP2 prevented p38-MAPK activation and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02396-1
更新日期:2001-05-04 00:00:00
abstract::A new type of mitogenic factor (protein) was purified from the culture supernatant of a strain of Streptococcus pyogenes by SP-Sephadex C-25 column chromatography, preparative isoelectric focusing and reversed-phase high-performance liquid chromatography. The purified factor, showing marked mitogenic activity in rabbi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81043-l
更新日期:1992-08-10 00:00:00
abstract::Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important prote...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.07.026
更新日期:2014-09-17 00:00:00
abstract::The transcriptional start site of the human cholecystokinin (CCK)-A receptor gene was determined by the Capsite Hunting method. Two sequence changes were detected, a G to T change in nucleotide -128, and an A to G change in nucleotide -81. The homozygote (T/T, G/G) was detected in 25 of 1296 individuals (1.9%) in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01080-2
更新日期:2000-01-28 00:00:00
abstract::Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02490-5
更新日期:2001-06-08 00:00:00
abstract::Elongation factors EF-G and EF-Tu are structural homologues and share near-identical binding sites on the ribosome, which encompass the GTPase-associated centre (GAC) and the sarcin-ricin loop (SRL). The SRL is fixed structure in the ribosome and contacts elongation factors in the vicinity of their GTP-binding site. I...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.09.010
更新日期:2005-10-24 00:00:00
abstract::We introduced rat ornithine transcarbamylase (OTC) gene into OTC-deficient spf-ash mice by mating spf-ash heterozygotes with transgenic mice which carried recombinant DNA composed of 1.3 kb of the 5' flanking region of the gene fused onto rat OTC cDNA. The liver OTC activity of hemizygous spf-ash mice which carried th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80148-v
更新日期:1991-02-25 00:00:00
abstract::The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02508-5
更新日期:2002-04-10 00:00:00
abstract::The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02641-8
更新日期:2002-04-24 00:00:00
abstract::The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following eit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80927-b
更新日期:1990-07-16 00:00:00
abstract::Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81242-z
更新日期:1991-09-23 00:00:00
abstract::VirG, PhoB, and OmpR are positive regulators of the virulence, phosphate, and osmolarity regulons, respectively. Their target genes are attended with one or more significant blocks of similar 6-bp sequences in their regulatory regions. The distance between the block(s) and transcriptional start site varies with the ta...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(90)80691-b
更新日期:1990-04-09 00:00:00
abstract::The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.067
更新日期:2006-01-09 00:00:00
abstract::MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:
更新日期:2013-11-29 00:00:00
abstract::The astroglial growth factor (AGF), which induces a characteristic morphological change in cultured rat astroglial cells and stimulates their proliferation, was purified to homogeneity from bovine brain. Two different methods were used, the second one including heparin-Sepharose affinity chromatography. AGF is actuall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80851-6
更新日期:1985-09-09 00:00:00
abstract::When rat pancreatic islets were incubated with 10(-8) M arginine vasopressin in the presence of 15 mM glucose there was a pronounced inhibition of insulin release in comparison with controls. This inhibitory effect appeared to be specific for vasopressin since it was antagonised by vasopressin antibody. Moreover, pert...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80720-8
更新日期:1988-04-11 00:00:00
abstract::This paper reviews our present knowledge of sphingomyelinases as enzymes, and as enzymes acting on a membrane constituent lipid, sphingomyelin. Six types of sphingomyelinases are considered, namely acidic, secretory, Mg(2+)-dependent neutral, Mg(2+)-independent neutral, alkaline, and bacterial enzymes with both phosph...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03482-8
更新日期:2002-10-30 00:00:00
abstract::Redox homeostasis is a prerequisite for survival of the pathogen Mycobacterium tuberculosis (Mtb) which employs the low molecular weight thiol mycothiol (MSH). The Mycobacterial NADPH-dependent mycothione reductase (MtMtr), composed of an NADPH-, FAD-, and a dimerization-domain connected by linkers, regulates the bala...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12984
更新日期:2018-02-01 00:00:00
abstract::Regulatory divergence is likely a major driving force in evolution. Comparative genomics is being increasingly used to infer the evolution of gene regulation. Ascomycota fungi are uniquely suited among eukaryotes for regulatory evolution studies, due to broad phylogenetic scope, many sequenced genomes, and tractabilit...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.032
更新日期:2009-12-17 00:00:00
abstract::The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00320-8
更新日期:1999-04-01 00:00:00
abstract::Exposing a macrophage-like murine cell line to copper-oxidised low-density lipoprotein led to DNA fragmentation which was inhibited by the putative Ca2+/Mg2+ endonuclease inhibitor, zinc sulphate. DNA fragmentation preceded loss of membrane impermeability. These results suggest that apoptosis may be a mechanism of mac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80635-8
更新日期:1993-10-18 00:00:00
abstract::Human hepatocellular carcinoma (HCC) is one of the most common tumors worldwide, in which the genetic mechanisms of oncogenesis are still unclear. To investigate whether the genomic DNA copy number alterations may contribute to primary HCC, the cDNA microarray-based comparative genomic hybridization (CGH) analysis was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.032
更新日期:2006-06-26 00:00:00
abstract::In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81808-d
更新日期:1993-07-12 00:00:00
abstract::An experimental procedure is described for the reproducible production of hybridoma cells using the electrofusion technique. High yields can be obtained when fusion is performed in isotonic inositol solutions containing Ca2+ and Mg2+ in a ratio of 1:5 in the millimolar range. The hybridoma cells are transferred 10 min...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80315-x
更新日期:1985-03-25 00:00:00
abstract::Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01463-5
更新日期:1999-11-19 00:00:00
abstract::Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81055-x
更新日期:1988-12-05 00:00:00
abstract::We show that the calcium-mimic dye, Stains-all, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP). These peptides bind the dye in their calcium-binding sites, and induce a circular dichroism (CD) band in the bo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81761-n
更新日期:1993-07-12 00:00:00
abstract::Photosynthetic machinery requires balanced distribution of excitation energy from the light-harvesting complexes to photosystems. The efficiency of light-harvesting is regulated by thermal dissipation of excess energy, while the distribution of energy between photosystems is dependent on STN7 kinase and phosphorylatio...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.07.035
更新日期:2012-08-31 00:00:00
abstract::The structure of GE82832, a translocation inhibitor produced by a soil microorganism, is shown to be highly related to that of dityromycin, a bicyclodecadepsipeptide antibiotic discovered long ago whose characterization had never been pursued beyond its structural elucidation. GE82832 and dityromycin were shown to int...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.040
更新日期:2012-09-21 00:00:00
abstract::Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.11.029
更新日期:2014-01-21 00:00:00