Fungal regulatory evolution: cis and trans in the balance.

abstract：:DNA-cytochrome c complexes adsorbed on carbon-coated mica surfaces were directly imaged by atomic force microscopy in air using commercially available cantilevers, with a routine resolution of 6 nm. Images of M13 phage DNA and M13-DNA polymerase complex are also shown. ...

journal_title：FEBS letters

authors： Yang J,Takeyasu K,Shao Z

更新日期：1992-04-20 00:00:00

abstract：:A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting ...

journal_title：FEBS letters

authors： Carissimi C,Baccon J,Straccia M,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

更新日期：2005-04-25 00:00:00

abstract：:The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...

journal_title：FEBS letters

authors： Evans LD,Roszak AW,Noble LJ,Robinson DA,Chalk PA,Matthews JL,Coggins JR,Price NC,Lapthorn AJ

更新日期：2002-10-23 00:00:00

abstract：:Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

journal_title：FEBS letters

authors： Austin RC,Rachubinski RA,Blajchman MA

更新日期：1991-03-25 00:00:00

abstract：:Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...

journal_title：FEBS letters

authors： Tachibanaki S,Imai H,Terakita A,Shichida Y

更新日期：1998-03-20 00:00:00

abstract：:Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

journal_title：FEBS letters

authors： Afanas'ev IB,Ostrachovich EA,Korkina LG

更新日期：1998-03-27 00:00:00

abstract：:Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

journal_title：FEBS letters

authors： Lovrić J,Bischof O,Moelling K

更新日期：1994-04-18 00:00:00

abstract：:Vacuolar tandem-pore channels could not be analysed in Xenopus oocytes so far, due to misguided translocation. Owing to the conservation of their pore regions, we were able to prepare functional pore-chimeras between the plasma membrane localised TPK4 and vacuolar TPKs. Thereby, we found evidence that TPK2, TPK3 and T...

journal_title：FEBS letters

authors： Marcel D,Müller T,Hedrich R,Geiger D

更新日期：2010-06-03 00:00:00

abstract：:Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...

journal_title：FEBS letters

authors： Cuenda A,Centeno F,Gutierrez-Merino C

更新日期：1991-06-03 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-regulated nuclear hormone receptor, plays critical roles in metabolism and adipogenesis. PPARγ ligands such as thiazolidinediones (TZDs) exert insulin sensitizing and anti-inflammatory effects primarily through action on adipocytes, and are thus widely...

journal_title：FEBS letters

authors： Sugii S,Evans RM

更新日期：2011-07-07 00:00:00

abstract：:The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physi...

journal_title：FEBS letters

authors： Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen P

更新日期：1994-03-14 00:00:00

abstract：:The alpha-subunit of the TRAP complex (TRAP alpha) is a single-spanning membrane protein of the endoplasmic reticulum (ER) which is found in proximity of nascent polypeptide chains translocating across the membrane. Here, we demonstrate the widespread occurrence of TRAP alpha in eukaryotes as indicated by its existenc...

journal_title：FEBS letters

authors： Hartmann E,Prehn S

更新日期：1994-08-08 00:00:00

abstract：:Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

journal_title：FEBS letters

authors： Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

更新日期：1987-06-29 00:00:00

abstract：:Chimeric proteins composed of ricin toxin A chain (RTA) and staphylococcal protein A (PA) have been produced in E. coli. Constructs consisting of N-terminal RTA and C-terminal PA (RTA-PA) or N-terminal PA and C-terminal (PA-RTA) were capable of binding to immunoglobulin G (via PA) and of specifically depurinating 28 S...

journal_title：FEBS letters

authors： O'Hare M,Brown AN,Hussain K,Gebhardt A,Watson G,Roberts LM,Vitetta ES,Thorpe PE,Lord JM

更新日期：1990-10-29 00:00:00

abstract：:Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...

journal_title：FEBS letters

authors： Steiner H,Pesold B,Haass C

更新日期：1999-12-17 00:00:00

abstract：:The conformation of the glycine-rich loop of adenylate kinase is described in detail. It forms a giant anion hole for a sulfate ion, which presumably mimicks a nucleotide phosphoryl group. This loop had been called flexible, because at pH values of 6 or below it is displaced in the crystal. In the region of this loop ...

journal_title：FEBS letters

authors： Dreusicke D,Schulz GE

更新日期：1986-11-24 00:00:00

abstract：:The amino acid sequence of ubiquitin from Entamoeba histolytica, as deduced from a cDNA nucleotide sequence, deviated at six positions from the consensus of all other known ubiquitins (ranging from Trypanosoma cruzi to Homo sapiens). The corresponding residues were scattered over the primary sequence, but came close t...

journal_title：FEBS letters

authors： Wöstmann C,Tannich E,Bakker-Grunwald T

更新日期：1992-08-10 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:Pro-apoptotic functions of the BH3-only protein BAD are negatively regulated by survival signal-mediated phosphorylation at several serine residues. Recently, we found that the mutant BAD (BADD119G) with an amino acid substitution of Asp (Asp119 to Gly) within the BH3 domain displays strong pro-apoptotic activity in s...

journal_title：FEBS letters

authors： Adachi M,Zhang YB,Imai K

更新日期：2003-09-11 00:00:00

abstract：:FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

journal_title：FEBS letters

authors： Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

更新日期：1990-02-12 00:00:00

abstract：:The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

journal_title：FEBS letters

authors： Wei AZ,Mayr I,Bode W

更新日期：1988-07-18 00:00:00

abstract：:Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains w...

journal_title：FEBS letters

authors： Reynolds PE,Brown DF

更新日期：1985-11-11 00:00:00

abstract：:H2O2 addition to the oxidized cytochrome c oxidase reconstituted in liposomes brings about a red shift of the Soret band of the enzyme and an increased absorption in the visible region with two distinct peaks at approximately 570 and 605 nm. Throughout pH range 6-8.5, the spectral changes at 570 nm and in the Soret ba...

journal_title：FEBS letters

authors： Vygodina T,Konstantinov AA

更新日期：1987-07-27 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii were used to determine the role of two energy-dissipating systems, i.e., a free fatty acid (FFA)-activated, purine nucleotide-inhibited uncoupling protein (AcUCP) and a FFA-insensitive, purine nucleotide-activated ubiquinol alternative oxidase (AcAOX), in decreasing reac...

journal_title：FEBS letters

authors： Czarna M,Jarmuszkiewicz W

更新日期：2005-06-06 00:00:00

abstract：:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...

journal_title：FEBS letters

authors： Jornot L,Petersen H,Junod AF

更新日期：1997-10-27 00:00:00

abstract：:A protein factor which inhibited adenylate cyclase was purified to apparent homogeneity from rat brain and identified as the beta gamma-subunits of the GTP-binding regulatory proteins of adenylate cyclase. (i) The beta gamma-subunits (protein factor) inhibited the partially purified catalytic unit of adenylate cyclase...

journal_title：FEBS letters

authors： Enomoto K,Asakawa T

更新日期：1986-06-23 00:00:00

abstract：:A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...

journal_title：FEBS letters

authors： Chang SF,Netter HJ,Will H

更新日期：1991-09-02 00:00:00

abstract：:The formation of cytoplasmic inclusion bodies by Escherichia coli overproducing Clostridium thermocellum endoglucanase D (EGD) was investigated. EGD was found in inclusion bodies as a 68 kDa form, whereas the size of the cytoplasmic form was 65 kDa. Upon solubilization with urea followed by dialysis, the 68 kDa form w...

journal_title：FEBS letters

authors： Tokatlidis K,Dhurjati P,Millet J,Béguin P,Aubert JP

更新日期：1991-04-22 00:00:00

abstract：:Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC-conditioned medium significantly attenuated proliferation of CFB compared with CFB-conditioned medium. MSC-conditioned me...

journal_title：FEBS letters

authors： Ohnishi S,Sumiyoshi H,Kitamura S,Nagaya N

更新日期：2007-08-21 00:00:00

abstract：:Treatment of human neutrophils with triphenyltin chloride (TPTCl)-inhibited superoxide (O-2) production stimulated with phorbol myristate acetate (PMA). TPTCl was more potent as inhibitor of O-2 production than other phenyltin compounds. The O-2 production by the xanthine oxidase-acetaldehyde system was not inhibited ...

journal_title：FEBS letters

authors： Matsui H,Wada O,Ushijima Y,Akuzawa T

更新日期：1983-12-12 00:00:00