Abstract:
:Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with reduced electrophoretic mobility, p102Gag-Mil. Appearance of p102Gag-Mil leads to a reduced association with p89 indicating that p102 is not associated with p89. Microsequencing of p89 isolated from immunoprecipitates of Gag-Mil identified the protein as the quail homologue of chicken hsp90. Our results show that p100Gag-Mil is associated with hsp90 with a high stoichiometry and that upshifted p102Gag-Mil is released from the complex with hsp90.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lovrić J,Bischof O,Moelling Kdoi
10.1016/0014-5793(94)80598-9subject
Has Abstractpub_date
1994-04-18 00:00:00pages
15-21issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)80598-9journal_volume
343pub_type
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