15N and 1H NMR evidence for multiple conformations of the complex of dihydrofolate reductase with its substrate, folate.

abstract：:FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

journal_title：FEBS letters

authors： Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

更新日期：1990-02-12 00:00:00

abstract：:Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...

journal_title：FEBS letters

authors： Jones PM,Stutchfield J,Howell SL

更新日期：1985-10-21 00:00:00

abstract：:Copper(II) was substituted for manganese(III) in Bacillus stearothermophilus Mn-superoxide dismutase. The (EPR) spectrum of the Cu(II) is distinctly rhombic, overlapping that of Cu(II) replacing zinc in copper/zinc superoxide dismutase. The copper-cyanide complex of the bacterial enzyme gives an EPR spectrum nearly id...

journal_title：FEBS letters

authors： Bannister JV,Desideri A,Rotilio G

更新日期：1985-08-19 00:00:00

abstract：:LEA (late embryogenesis abundant) proteins are intrinsically disordered proteins that contribute to stress tolerance in plants and invertebrates. Here we show that, when both plant and animal LEA proteins are co-expressed in mammalian cells with self-aggregating polyglutamine (polyQ) proteins, they reduce aggregation ...

journal_title：FEBS letters

authors： Liu Y,Chakrabortee S,Li R,Zheng Y,Tunnacliffe A

更新日期：2011-02-18 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:We reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (G3DH) from marine bacterium Halomonas sp. alpha-15 cells. The product of G3DH enzymatic conversion was 3,3'-diketotrehalose (3,3'dkT), a novel trehalose derivative of which bo...

journal_title：FEBS letters

authors： Sode K,Akaike E,Sugiura H,Tsugawa W

更新日期：2001-01-26 00:00:00

abstract：:Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

journal_title：FEBS letters

authors： Gether U,Marray T,Schwartz TW,Johansen TE

更新日期：1992-01-27 00:00:00

abstract：:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...

journal_title：FEBS letters

authors： Wernet W,Flockerzi V,Hofmann F

更新日期：1989-07-17 00:00:00

abstract：:It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CB...

journal_title：FEBS letters

authors： Wolf-Watz M,Bäckström S,Grundström T,Sauer U,Härd T

更新日期：2001-01-12 00:00:00

abstract：:Here, we report the volume and enthalpy changes accompanying CO photodissociation from the mixed valence form of cytochrome bo3 oxidase from Escherichia coli. The results of photoacoustic calorimetry indicate two kinetic phases with distinct volume and enthalpy changes accompanying CO photodissociation from heme o3 an...

journal_title：FEBS letters

authors： Miksovská J,Gennis RB,Larsen RW

更新日期：2005-06-06 00:00:00

abstract：:Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...

journal_title：FEBS letters

authors： Ohsawa S,Miura M

更新日期：2006-10-30 00:00:00

abstract：:Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L-glutamate by a Na(+)-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-threo-3-hydroxyaspartate and L-trans-pyrrolidine-2...

journal_title：FEBS letters

authors： Balcar VJ,Shen J,Bao S,King NJ

更新日期：1994-02-14 00:00:00

abstract：:The placenta comprises a highly specialized trophoblast layer, which arises from the embryo and differentiates during embryonic development to perform specialized functions, e.g., synthesis of pregnancy-associated hormones, growth factors and cytokines. As there is no evidence of maternal acute-phase protein transplac...

journal_title：FEBS letters

authors： Kovacevic A,Hammer A,Sundl M,Pfister B,Hrzenjak A,Ray A,Ray BK,Sattler W,Malle E

更新日期：2006-01-09 00:00:00

abstract：:Plastoquinone-9 (PQ-9)-depleted PSII reaction center core complex, consisting of CP47/D1/D2/Cytb-559/I, was isolated from spinach PSII particles. PQ-9, lipids and several proteins were extracted from the original PSII particles and separated by several steps of chromatography to be reconstituted into the isolated comp...

journal_title：FEBS letters

authors： Kitamura K,Ozawa S,Shiina T,Toyoshima Y

更新日期：1994-10-31 00:00:00

abstract：:The kinetic constants of internalization of asialoorosomucoid were determined for normal and jejuno-ileal by-passed rat hepatocytes. In by-passed rats the maximum velocity of asialoorosomucoid internalization is decreased 3-fold, without any modification of apparant constant of internalization. Moreover, the rate cons...

journal_title：FEBS letters

authors： Serbource-Goguel N,Dodeur M,Borel B,Feger J,Agneray J,Durand G

更新日期：1985-04-22 00:00:00

abstract：:Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

journal_title：FEBS letters

authors： Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

更新日期：1991-06-24 00:00:00

abstract：:An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

journal_title：FEBS letters

authors： Alkhatib SG,Landry JW

更新日期：2011-10-20 00:00:00

abstract：:Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

journal_title：FEBS letters

authors： Kawade R,Kuroda D,Tsumoto K

更新日期：2020-03-01 00:00:00

abstract：:The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

journal_title：FEBS letters

authors： Olsen J,Laustsen L,Troelsen J

更新日期：1994-04-11 00:00:00

abstract：:Recent studies have revealed the presence of an atypical progesterone receptor on the plasma membrane of a relatively small subpopulation of human sperm cells. In this study, we show that the receptor is present in many cells in a latent form and that the receptor function in these cells is switched on asynchronously ...

journal_title：FEBS letters

authors： Mendoza C,Tesarik J

更新日期：1993-09-06 00:00:00

abstract：:The amino acid sequence of protein Z has been determined from sequence analysis performed on fragments obtained by chemical and enzymatic degradations. The polypeptide consists of a single chain containing 396 amino acid residues (Mr 43 677). Comparison with the vitamin K-dependent plasma proteins reveals an extensive...

journal_title：FEBS letters

authors： Højrup P,Jensen MS,Petersen TE

更新日期：1985-05-20 00:00:00

abstract：:A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

journal_title：FEBS letters

authors： Palomäki A,Malminiemi K,Metsä-Ketelä T

更新日期：1997-06-30 00:00:00

abstract：:Luminescence of Tb3+ ions bound to a calmodulin fragment has been studied. It is shown that during their lifetime excited ions dissociate from the peptide. If concentration of free peptide is high enough they can be coordinated again. As a consequence, observed terbium luminescence lifetime and intensity depends not o...

journal_title：FEBS letters

authors： Dadlez M,Góral J,Bierzyński A

更新日期：1991-04-22 00:00:00

abstract：:Two alpha-type CK2-activated PKAs (CK2-aPKAIalpha and CK2-aPKAIIalpha) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcor...

journal_title：FEBS letters

authors： Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

更新日期：2006-02-06 00:00:00

abstract：:Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

journal_title：FEBS letters

authors： Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

更新日期：2001-03-09 00:00:00

abstract：:1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...

journal_title：FEBS letters

authors： Hamada M,Nishio K,Doe M,Usuki Y,Tanaka T

更新日期：2002-03-13 00:00:00

abstract：:We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

journal_title：FEBS letters

authors： Fernández A

更新日期：1992-02-03 00:00:00

abstract：:We have isolated cDNAs encoding the glucocorticoid receptor from an androgen-dependent mouse tumor, Shionogi Carcinoma 115. The nucleotide sequence of the receptor revealed two different forms, designated as SC-GR I and SC-GR II. Both forms have a one-base substitution in the DNA binding domain of the wild-type mouse ...

journal_title：FEBS letters

authors： Kasai Y

更新日期：1990-11-12 00:00:00

abstract：:Nuclear pore complexes (NPC) form nuclear pores that cross the nuclear envelope and allow molecules to transport between the nucleus and the cytoplasm. We solved the crystal structure of human Nup43 (hNUP43), an important component in the Nup107 subcomplex of NPC. hNup43 adopts a seven-bladed β-propeller fold. We conf...

journal_title：FEBS letters

authors： Xu C,Li Z,He H,Wernimont A,Li Y,Loppnau P,Min J

更新日期：2015-10-24 00:00:00

abstract：:The number of proteins found in body fluids and at cell surfaces, which are known to display carbohydrate-binding properties, continues to increase rapidly. In these proteins, in addition to a domain associated with lectin properties, one or more, non-lectin domains are present. It is possible that binding of sugar re...

journal_title：FEBS letters

authors： Thiel S,Reid KB

更新日期：1989-06-19 00:00:00