HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

abstract：:The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...

journal_title：FEBS letters

authors： Fryer LG,Parbu-Patel A,Carling D

更新日期：2002-11-06 00:00:00

abstract：:Phosphorylation of fibronectin was studied in quiescent vs growing cells from several species. Fibronectin secreted by actively growing cells exhibits a significantly higher level of phosphorylation than does the fibronectin secreted by quiescent cells of the same species. ...

journal_title：FEBS letters

authors： Ali IU

更新日期：1983-01-10 00:00:00

abstract：:In vertebrate photoreceptors the soluble protein arrestin (45 kDa) is involved in controlling the light dependent activity of receptor proteins such as transducin or the cGMP-phosphodiesterase. Arrestin has further been identified as the retinal-S-antigen which is assumed to cause the autoimmune disease uveitis. In a ...

journal_title：FEBS letters

authors： Glitscher W,Rüppel H

更新日期：1991-05-06 00:00:00

abstract：:Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...

journal_title：FEBS letters

authors： Engels F,Willems H,Nijkamp FP

更新日期：1986-12-15 00:00:00

abstract：:Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed tha...

journal_title：FEBS letters

authors： Jian D,Aili Z,Xiaojia B,Huansheng Z,Yun H

更新日期：2010-12-01 00:00:00

abstract：:The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when co...

journal_title：FEBS letters

authors： Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T

更新日期：1996-03-04 00:00:00

abstract：:Calpastatin molecule contains four repeated inhibition domains, each having highly conserved internal regions A, B and C. The synthetic oligopeptides of regions A and C had no calpain inhibition activity while region B oligopeptide showed weak inhibition activity. Real-time biomolecular interaction analysis using a BI...

journal_title：FEBS letters

authors： Takano E,Ma H,Yang HQ,Maki M,Hatanaka M

更新日期：1995-03-27 00:00:00

abstract：:We previously described that NO. leads to destruction of ZnS clusters and release of Zn2+ from various proteins including zinc finger transcription factors. To assess the relevance in living cells, we investigated, whether exogenous NO. leads to an increase of cytoplasmic and nuclear free Zn2+. L929 cells, mouse splen...

journal_title：FEBS letters

authors： Berendji D,Kolb-Bachofen V,Meyer KL,Grapenthin O,Weber H,Wahn V,Kröncke KD

更新日期：1997-03-17 00:00:00

abstract：:The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. litt...

journal_title：FEBS letters

authors： Sanchis V,Chaufaux J,Pauron D

更新日期：1994-10-24 00:00:00

abstract：:The multiline signal of the S(2) state in Photosystem II was measured both in frozen-solution and single-crystal preparations from the cyanobacterium Thermosynechococcus elongatus. The frozen-solution EPR spectrum shows a gaussian-like line shape without any resolution of Mn hyperfine couplings. This line shape can be...

journal_title：FEBS letters

authors： Teutloff C,Kessen S,Kern J,Zouni A,Bittl R

更新日期：2006-06-26 00:00:00

abstract：:Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

journal_title：FEBS letters

authors： Kleuser B,Maceyka M,Milstien S,Spiegel S

更新日期：2001-08-10 00:00:00

abstract：:Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...

journal_title：FEBS letters

authors： Huang CH,Chen YC,Chen CC

更新日期：2013-06-19 00:00:00

abstract：:Melittin, deuteromethylated on each of the four amino groups (Gly-1 N alpha and Lys-7, 21, and 23 N epsilon), was prepared by reductive methylation using deuteroformaldehyde and NaBD3CN. Deuterium NMR spectra were obtained for the modified peptide (D-melittin) bound to phospholipid bilayers and erythrocyte ghosts. D-M...

journal_title：FEBS letters

authors： Dempsey CE,Cryer GD,Watts A

更新日期：1987-06-22 00:00:00

abstract：:FabH (beta-ketoacyl-acyl carrier protein synthase III) is unique in that it initiates fatty acid biosynthesis, is inhibited by long-chain fatty acids providing means for feedback control of the process, and dictates the fatty acid profile of the organism by virtue of its substrate specificity. We report the crystal st...

journal_title：FEBS letters

authors： Gajiwala KS,Margosiak S,Lu J,Cortez J,Su Y,Nie Z,Appelt K

更新日期：2009-09-03 00:00:00

abstract：:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks ...

journal_title：FEBS letters

authors： Loukin SH,Su Z,Kung C

更新日期：2009-02-18 00:00:00

abstract：:Activated p38gamma MAP kinase exhibited significant basal ATPase activity in the absence of a kinase substrate, and addition of a phosphoacceptor substrate increased k(cat)/K(m)20-fold. AMP-PCP was competitive with ATP binding and non-competitive with phosphoacceptor substrate binding. The nucleotide binding site affi...

journal_title：FEBS letters

authors： Fox T,Fitzgibbon MJ,Fleming MA,Hsiao HM,Brummel CL,Su MS

更新日期：1999-11-19 00:00:00

abstract：:The synthetic cRNA encoding for the major T lymphocyte K+ channel (Kv1.3) was injected into Xenopus fertilized eggs. Somites from embryos of stage 20-22 (about 40 h post-fertilization at 19 degrees C) were dissociated and myotomal muscle cells were cultured in vitro for 2 days. The whole cell configuration of the tigh...

journal_title：FEBS letters

authors： Honoré E,Guillemare E,Lesage F,Barhanin J,Lazdunski M

更新日期：1994-07-18 00:00:00

abstract：:Two different isoforms of subunit VIIa have been found in cytochrome c oxidase isolated from human skeletal muscle. The first 22 residues of the N-terminal amino acid sequences showed 5 differences. Our results provide the first conclusive evidence for the existence of cytochrome c oxidase isoenzymes in man. Since the...

journal_title：FEBS letters

authors： Van Beeumen JJ,Van Kuilenburg AB,Van Bun S,Van den Bogert C,Tager JM,Muijsers AO

更新日期：1990-04-24 00:00:00

abstract：:Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ ...

journal_title：FEBS letters

authors： Webster GT,Tilley L,Deed S,McNaughton D,Wood BR

更新日期：2008-04-02 00:00:00

abstract：:The gene for human tetranectin was isolated from a genomic library with a mixture of degenerate oligonucleotide probes. The gene is about 12 kbp and contains two intervening sequences. The gene encodes a protein of 202 amino acid residues, with a signal peptide of 21 amino acid residues, followed by the tetranectin se...

journal_title：FEBS letters

authors： Berglund L,Petersen TE

更新日期：1992-08-31 00:00:00

abstract：:A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...

journal_title：FEBS letters

authors： Murakami R,Ifuku K,Takabayashi A,Shikanai T,Endo T,Sato F

更新日期：2002-07-17 00:00:00

abstract：:Comparative allelotyping of the short arm of human chromosome 3 (3p) in four types of epithelial carcinomas was performed using an identical set of polymorphic markers. In total, 117 samples of non-papillary renal cell carcinoma (RCC), non-small cell lung carcinoma (NSCLC), carcinoma of uterine cervix (CC), and breast...

journal_title：FEBS letters

authors： Braga E,Pugacheva E,Bazov I,Ermilova V,Kazubskaya T,Mazurenko N,Kisseljov F,Liu J,Garkavtseva R,Zabarovsky E,Kisselev L

更新日期：1999-07-09 00:00:00

abstract：:Aquaporin-4, a predominant water channel in the central nervous system, has two isoforms, M1 and M23, whose transcripts are driven by distinct promoters. Using a reporter assay, we found that a fragment located between exons 0 and 1 of the mouse aquaporin-4 gene, which had been thought to be the promoter for M23, lack...

journal_title：FEBS letters

authors： Abe Y,Goda W,Ikeshima-Kataoka H,Yasui M

更新日期：2017-12-01 00:00:00

abstract：:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)...

journal_title：FEBS letters

authors： Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y

更新日期：1996-04-15 00:00:00

abstract：:The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

journal_title：FEBS letters

authors： Norris MD,Stewart BW

更新日期：1988-02-15 00:00:00

abstract：:Muscarinic K+ channels (IK(ACh)) in native atrial myocytes are activated by betagamma subunits of pertussis toxin (Ptx)-sensitive heterotrimeric G proteins coupled to different receptors. betagamma subunits of Ptx-insensitive Gs, coupled to beta-adrenergic receptors, do not activate native IK(ACh). In atrial myocytes ...

journal_title：FEBS letters

authors： Bender K,Wellner-Kienitz MC,Meyer T,Pott L

更新日期：1998-11-13 00:00:00

abstract：:Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

journal_title：FEBS letters

authors： Döbbeling U,Berchtold MW

更新日期：1996-08-05 00:00:00

abstract：:We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

journal_title：FEBS letters

authors： Sakaguchi M,Honda S,Ozawa M,Nishimura O

更新日期：1988-03-28 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00

abstract：:The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...

journal_title：FEBS letters

authors： Brzezinski P,Thörnström PE,Malmström BG

更新日期：1986-01-01 00:00:00