Study of hepatic binding protein activity in jejuno-ileal by-passed rat hepatocytes.

abstract：:Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...

journal_title：FEBS letters

authors： Terziyska N,Lutz T,Kozany C,Mokranjac D,Mesecke N,Neupert W,Herrmann JM,Hell K

更新日期：2005-01-03 00:00:00

abstract：:The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C-terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: His-Ala-Asp-Gly-Val-...

journal_title：FEBS letters

authors： Tatemoto K,Carlquist M,McDonald TJ,Mutt V

更新日期：1983-03-21 00:00:00

abstract：:Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

journal_title：FEBS letters

authors： Hall M,Taylor SJ,Saggerson ED

更新日期：1985-01-07 00:00:00

abstract：:A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pr...

journal_title：FEBS letters

authors： Minakuchi K,Yabushita T,Masumura T,Ichihara K,Tanaka K

更新日期：1994-01-10 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:We have studied the nuclear magnetic resonance solution secondary structure of the N-terminal region in human erythroid alpha-spectrin using a recombinant model peptide of alpha-spectrin consisting of residues 1-156. Pulsed field gradient diffusion coefficient measurements show that the model peptide exists as a monom...

journal_title：FEBS letters

authors： Park S,Johnson ME,Fung LW

更新日期：2000-11-17 00:00:00

abstract：:Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(...

journal_title：FEBS letters

authors： Kiss AL,Szeltner Z,Fülöp V,Polgár L

更新日期：2004-07-30 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

journal_title：FEBS letters

authors： Feuerbach D,Feyen JH

更新日期：1997-01-27 00:00:00

abstract：:Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...

journal_title：FEBS letters

authors： Chemin J,Monteil A,Briquaire C,Richard S,Perez-Reyes E,Nargeot J,Lory P

更新日期：2000-07-28 00:00:00

abstract：:Epiregulin (EPR), a novel member of epidermal growth factor (EGF) family, is a ligand for ErbB-1 and ErbB-4 receptors. The binding affinity of EPR for the receptors is lower than those of other EGF-family ligands. The solution structure of EPR was determined using two-dimensional nuclear magnetic resonance spectroscop...

journal_title：FEBS letters

authors： Sato K,Nakamura T,Mizuguchi M,Miura K,Tada M,Aizawa T,Gomi T,Miyamoto K,Kawano K

更新日期：2003-10-23 00:00:00

abstract：:Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...

journal_title：FEBS letters

authors： Hofmann S,Düsterhöft S,Pette D

更新日期：1988-10-10 00:00:00

abstract：:We have investigated whether the expression in Arabidopsis thaliana seeds of a transgene (the Phaseolus vulgaris arcelin (arc)5-I gene) could be enhanced by the simultaneous introduction of an antisense gene for an endogenous seed storage protein (2S albumin). Seeds of plants transformed with both the arc5-I gene and ...

journal_title：FEBS letters

authors： Goossens A,Van Montagu M,Angenon G

更新日期：1999-07-30 00:00:00

abstract：:32P-Labelled tRNA was isolated from uninfected and phage phi 149-infected Vibrio cholerae cells. These tRNA preparations were then hybridised with DNA isolated from phage phi 149. Significant hybridisation was observed only with tRNA from phage phi 149-infected cells. This strongly suggests that infection of classical...

journal_title：FEBS letters

authors： Ghosh RK,Guhathakurta I

更新日期：1983-10-03 00:00:00

abstract：:The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

journal_title：FEBS letters

authors： Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

更新日期：2006-11-27 00:00:00

abstract：:We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...

journal_title：FEBS letters

authors： Sakai H,Suzuki T,Maeda M,Takahashi Y,Horikawa N,Minamimura T,Tsukada K,Takeguchi N

更新日期：2004-04-09 00:00:00

abstract：:Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...

journal_title：FEBS letters

authors： Fujisawa T,Kostyukova A,Maéda Y

更新日期：2001-06-01 00:00:00

abstract：:BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

journal_title：FEBS letters

authors： Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

更新日期：1996-12-09 00:00:00

abstract：:The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

journal_title：FEBS letters

authors： Bondy SC,Marwah S

更新日期：1995-11-13 00:00:00

abstract：:Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

journal_title：FEBS letters

authors： Brown EM,Redgrave J,Thatcher J

更新日期：1984-09-17 00:00:00

abstract：:Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

journal_title：FEBS letters

authors： Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

更新日期：2005-02-28 00:00:00

abstract：:The band 3 protein-mediated, valinomycin-induced KCl efflux continues to increase with increasing [KCl] when the Cl-/Cl- equilibrium exchange becomes saturated. This suggests the existence of a band 3-mediated component of Cl- flux that contributes to the electrical conductance without being related to slippage; i.e.,...

journal_title：FEBS letters

authors： Kaplan JH,Pring M,Passow H

更新日期：1983-05-30 00:00:00

abstract：:Using PCR-based techniques, we have isolated and sequenced the full-length cDNAs that encode the human neuronal nAChR subunits alpha3,4,5,7 and beta2,3,4 in the neuroblastoma cell lines SH-SY5Y and/or IMR-32. The predicted nAChR beta3- and beta4-subunit proteins contain 458 and 498 amino acids, respectively. Except fo...

journal_title：FEBS letters

authors： Groot Kormelink PJ,Luyten WH

更新日期：1997-01-06 00:00:00

abstract：:Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

journal_title：FEBS letters

authors： Sasaki K,Yoshida H

更新日期：2019-09-01 00:00:00

abstract：:Islet-activating protein (IAP) suppressed Ca2+-induced histamine release and phospholipase A2 activation in Gpp(NH)p-loaded mast cells. Since the guanine nucleotide-binding protein involved in adenylate cyclase inhibition is known to lose its function upon being ADP-ribosylated by IAP, the nucleotide-binding protein i...

journal_title：FEBS letters

authors： Nakamura T,Ui M

更新日期：1984-08-06 00:00:00

abstract：:The increase of hepatic apolipoprotein B (apoB) mRNA editing during rat development was not affected by hypothyroidism. Furthermore, the addition of 3,3',5'-triiodothyronine (T3) to cultured hepatocytes taken from fetal, neonatal and adult rats had no effect on apoB mRNA editing. In contrast, dexamethasone markedly st...

journal_title：FEBS letters

authors： Lorentz A,Plonné D,Schulze HP,Dargel R

更新日期：1996-08-05 00:00:00

abstract：:Like glutathione or dithiothreitol, metallothionein effects the formation of pancreatic ribonuclease A from its S-sulfonated derivative catalyzed by protein disulfide isomerase. EDTA increases the yield of ribonuclease A activity recovery with metallothionein but does not affect the reaction with glutathione or dithio...

journal_title：FEBS letters

authors： Zhang S,Li J,Wang CC,Tsou CL

更新日期：1999-12-03 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:Stimulation of platelets results in the liberation of arachidonic acid (AA) which is further metabolized via the cyclooxygenase or lipoxygenase (LPG) pathway. We have examined the effect of inhibition of LPG on (i) the ADP-induced increase of cytoplasmic Ca2+ concentration and (ii) platelet aggregation. Lipoxygenase i...

journal_title：FEBS letters

authors： Borin ML,Pinelis VG,Ivanova MA,Kudinov YuV,Azizova OA,Markov CM,Khodorov BI

更新日期：1989-11-06 00:00:00

abstract：:Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...

journal_title：FEBS letters

authors： Wooff J,Pastushok L,Hanna M,Fu Y,Xiao W

更新日期：2004-05-21 00:00:00