Two naturally-occurring isoforms and their expression of a glucocorticoid receptor gene from an androgen-dependent mouse tumor.

abstract：:A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated p...

journal_title：FEBS letters

authors： Tsuchihara K,Wazawa T,Ishii Y,Yanagida T,Nishida R,Zheng XG,Ishiguro M,Yoshihara K,Hisatomi O,Tokunaga F

更新日期：2009-01-22 00:00:00

abstract：:For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...

journal_title：FEBS letters

authors： Holzer T,Liffers K,Rahm K,Trageser B,Ozbek S,Gradl D

更新日期：2012-06-04 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit the effects of proinflammatory cytokines such as interleukin-1beta (IL-1beta). This cytokine plays a key role in articular pathophysiologies by inducing the production of inflammatory mediators such as nitric oxide (NO) and...

journal_title：FEBS letters

authors： Boyault S,Bianchi A,Moulin D,Morin S,Francois M,Netter P,Terlain B,Bordji K

更新日期：2004-08-13 00:00:00

abstract：:Ca4.calmodulin (Ca4.CaM) inhibits the glycolytic enzyme phosphofructokinase, by preventing formation of its active tetramer. Fluorescence titrations show that the affinity of complex formation of Ca4.CaM with the key 21-residue target peptide increases 1000-fold from pH 9.0 to 4.8, suggesting the involvement of histid...

journal_title：FEBS letters

authors： Martin SR,Biekofsky RR,Skinner MA,Guerrini R,Salvadori S,Feeney J,Bayley PM

更新日期：2004-11-05 00:00:00

abstract：:A cDNA clone of about 2300 base pairs was prepared from the human osteosarcoma cell line U-2 OS by hybridization with a 22-mer oligonucleotide complementary to the NH2-terminus of PDGF-A. Restriction and sequence analysis showed that this clone contains the entire coding region for PDGF-A and a long 3'-untranslated re...

journal_title：FEBS letters

authors： Hoppe J,Schumacher L,Eichner W,Weich HA

更新日期：1987-11-02 00:00:00

abstract：:A collagenase cleaving native type I [14C]collagen but inactive against the synthetic substrate Pz-Pro-Leu-Gly-Pro-D-Arg was extracted from mineralized human dental tissue. The enzyme specifically degrades native collagen into characteristic products (3/4) and (1/4). Its apparent molecular mass of 68 kDa is relatively...

journal_title：FEBS letters

authors： Dumas J,Hurion N,Weill R,Keil B

更新日期：1985-07-22 00:00:00

abstract：:Recombinant Plasmodium falciparum glyoxalase I (PfGlx I) was characterized as monomeric Zn(2+)-containing enzyme of 44 kDa. The K(M) value of the methylglyoxal-glutathione adduct is 77+/-15 microM, the k(cat) value being 4000 min(-1) at 25 degrees C and pH 7.0. PfGlx I consists of two halves, each of which is homologo...

journal_title：FEBS letters

authors： Iozef R,Rahlfs S,Chang T,Schirmer H,Becker K

更新日期：2003-11-20 00:00:00

abstract：:Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like pr...

journal_title：FEBS letters

authors： Jirik FR,Janzen NM,Melhado IG,Harder KW

更新日期：1990-10-29 00:00:00

abstract：:We have shown by electrophoretic mobility shift assays that the nucleotide sequence CE-LPH1, centred at position -49 with respect to the transcription start site of the rat gene encoding intestinal lactase-phlorizin hydrolase, interacts in vitro with nuclear proteins present in the jejunum of suckling animals. Protein...

journal_title：FEBS letters

authors： Boukamel R,Freund JN

更新日期：1994-10-10 00:00:00

abstract：:Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possi...

journal_title：FEBS letters

authors： Pejler G

更新日期：1996-04-01 00:00:00

abstract：:Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

journal_title：FEBS letters

authors： Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

更新日期：1987-02-23 00:00:00

abstract：:We found that miR-96 is overexpressed in glioma, and its level inversely correlates with the survival of patients. The reduction in miR-96 abundance suppresses the proliferation and colony formation of glioma cells. The tumorigenicity of U-87 MG cells is reduced by miR-96 silencing. miR-96 contributes to the activatio...

journal_title：FEBS letters

authors： Yan Z,Wang J,Wang C,Jiao Y,Qi W,Che S

更新日期：2014-08-25 00:00:00

abstract：:In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...

journal_title：FEBS letters

authors： Peraldi P,Frödin M,Barnier JV,Calleja V,Scimeca JC,Filloux C,Calothy G,Van Obberghen E

更新日期：1995-01-09 00:00:00

abstract：:We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...

journal_title：FEBS letters

authors： Ko HM,Jung HH,Seo KH,Kang YR,Kim HA,Park SJ,Lee HK,Im SY

更新日期：2006-05-29 00:00:00

abstract：:Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...

journal_title：FEBS letters

authors： McCarthy L,Bentley-DeSousa A,Denoncourt A,Tseng YC,Gabriel M,Downey M

更新日期：2020-01-01 00:00:00

abstract：:alpha2-Macroglobulin was derivatized with a sulfhydryl cross-linker and sandwiched between a mica substrate and a silicon nitride tip, both coated with gold, of an atomic force microscope and force curve measurement was carried out. An extensive downward deflection of the cantilever was observed in the retracting real...

journal_title：FEBS letters

authors： Mitsui K,Hara M,Ikai A

更新日期：1996-04-29 00:00:00

abstract：:The plant toxin ricin has proven valuable as a membrane marker in studies of endocytosis as well as studies of different intracellular transport steps. The toxin, which consists of two polypeptide chains, binds by one chain (the B-chain) to both glycolipids and glycoproteins with terminal galactose at the cell surface...

journal_title：FEBS letters

authors： Sandvig K,van Deurs B

更新日期：1999-06-04 00:00:00

abstract：:The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...

journal_title：FEBS letters

authors： Fayet G,Aouani A,Hovsépian S

更新日期：1986-01-06 00:00:00

abstract：:Chronic neuroleptic treatment induces an increase in the density of D2 dopamine receptors in the striatum. The effect of prolonged administration of haloperidol on mRNA levels of the short (D2S) and long (D2L) isoforms of the D2 dopamine receptor and the D3 dopamine receptor in different brain regions was examined. Ha...

journal_title：FEBS letters

authors： Fishburn CS,David C,Carmon S,Fuchs S

更新日期：1994-02-14 00:00:00

abstract：:The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

journal_title：FEBS letters

authors： Galietta LJ,Haggie PM,Verkman AS

更新日期：2001-06-22 00:00:00

abstract：:Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...

journal_title：FEBS letters

authors： Mikulecky P,Andreeva E,Amara P,Weissenhorn W,Nicolet Y,Macheboeuf P

更新日期：2018-01-01 00:00:00

abstract：:In contrast to the preponderance of affected males in families with X-linked mental retardation, Rett syndrome (RTT) is a neurological disorder occurring almost exclusively in females. The near complete absence of affected males in RTT families has been explained by the lethal effect of an X-linked gene mutation in he...

journal_title：FEBS letters

authors： Orrico A,Lam C,Galli L,Dotti MT,Hayek G,Tong SF,Poon PM,Zappella M,Federico A,Sorrentino V

更新日期：2000-09-22 00:00:00

abstract：:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...

journal_title：FEBS letters

authors： Touqui L,Jandrot-Perrus M,Vargaftig BB

更新日期：1989-09-25 00:00:00

abstract：:The nucleotide sequences of the dnaQ genes from Salmonella typhimurium and Buchnera aphidicola, encoding the epsilon-subunit of the DNA polymerase III holoenzyme, have been determined. The Salmonella typhimurium dnaQ protein consists of 243 amino acid residues with a calculated molecular weight of 27224. The Buchnera ...

journal_title：FEBS letters

authors： Huang Y,Braithwaite DK,Ito J

更新日期：1997-01-02 00:00:00

abstract：:IRF-8/ICSBP and IRF-1 are IRF family members whose expression is induced in response to IFN-gamma in macrophages. IL-12 is a cytokine produced in macrophages that plays a critical role in host defense. IFN-gamma and bacterial lipopolysaccharide (LPS) induce IL-12p40 transcription, which is necessary for the production...

journal_title：FEBS letters

authors： Masumi A,Tamaoki S,Wang IM,Ozato K,Komuro K

更新日期：2002-11-06 00:00:00

abstract：:The process of cell reprogramming has been characterized considerably since the successful generation of induced pluripotent stem cells. However, the importance of cell-cell communications for cellular reprogramming remains largely unknown. Secreted factors, which are expressed and secreted during reprogramming, may i...

journal_title：FEBS letters

authors： Bansho Y,Lee J,Nishida E,Nakajima-Koyama M

更新日期：2017-06-01 00:00:00

abstract：:The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....

journal_title：FEBS letters

authors： Ohtani H,Itoh H,Shinmura T

更新日期：1992-06-22 00:00:00

abstract：:Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...

journal_title：FEBS letters

authors： Kido H,Fukusen N,Katunuma N

更新日期：1987-11-02 00:00:00

abstract：:Human ECE-1 is expressed in four isoforms with different tissue distribution and its mRNA and protein levels are altered under certain pathophysiological conditions. To investigate the transcriptional regulation of ECE-1, we studied the regulatory region of ECE-1c, the major ECE-1 isoform. A genomic clone comprising t...

journal_title：FEBS letters

authors： Funke-Kaiser H,Bolbrinker J,Theis S,Lemmer J,Richter CM,Paul M,Orzechowski HD

更新日期：2000-01-28 00:00:00

abstract：:Mg2+ -dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on...

journal_title：FEBS letters

authors： Siegrist J,Netzer J,Mordhorst S,Karst L,Gerhardt S,Einsle O,Richter M,Andexer JN

更新日期：2017-01-01 00:00:00