Identification and characterization of secreted factors that are upregulated during somatic cell reprogramming.


:The process of cell reprogramming has been characterized considerably since the successful generation of induced pluripotent stem cells. However, the importance of cell-cell communications for cellular reprogramming remains largely unknown. Secreted factors, which are expressed and secreted during reprogramming, may influence the reprogramming efficiency. Here, we have identified Sostdc1, Glb1l2, Fetub, Dpp4, Gdf3, Trh, and Tdgf1 as prominently upregulated secreted factors during reprogramming. Our detailed analysis reveals that these seven factors may be categorized into four groups based on their expression patterns in relation to the reprogramming stages. Remarkably, knockdown of Sostdc1, which is the most prominently upregulated factor and which is expressed earlier than the other six factors, results in reduced reprogramming efficiency, suggesting its involvement in the reprogramming process.




FEBS letters


Bansho Y,Lee J,Nishida E,Nakajima-Koyama M




Has Abstract


2017-06-01 00:00:00












  • Anti-digoxin scFv fragments expressed in bacteria and in insect cells have different antigen binding properties.

    abstract::A gene encoding a single-chain antibody fragment directed against digoxin (named 1C10 scFv) was cloned in two expression systems. For this purpose, a new baculovirus transfer cassette fully compatible with the procaryotic pHEN vector was constructed. Baculovirus production led to higher yield than did Escherichia coli...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lemeulle C,Chardès T,Montavon C,Chaabihi H,Mani JC,Pugnière M,Cerutti M,Devauchelle G,Pau B,Biard-Piechaczyk M

    更新日期:1998-02-20 00:00:00

  • TP63 and TP73 in cancer, an unresolved "family" puzzle of complexity, redundancy and hierarchy.

    abstract::TP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and intera...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Costanzo A,Pediconi N,Narcisi A,Guerrieri F,Belloni L,Fausti F,Botti E,Levrero M

    更新日期:2014-08-19 00:00:00

  • Loops linking secondary structure elements affect the stability of the molten globule intermediate state of apomyoglobin.

    abstract::Apomyoglobin is a widely used model for studying the molecular mechanisms of globular protein folding. This work aimed to analyze the effects of rigidity and length of loops linking protein secondary structure elements on the stability of the molten globule intermediate state. For this purpose, we studied folding/unfo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Majorina MA,Balobanov VA,Uversky VN,Melnik BS

    更新日期:2020-10-01 00:00:00

  • NMR studies of fluorophenylalanine-containing carbonic anhydrase.

    abstract::Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of mos...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gamcsik MP,Gerig JT

    更新日期:1986-02-03 00:00:00

  • Metabolic profiling using combined GC-MS and LC-MS provides a systems understanding of aristolochic acid-induced nephrotoxicity in rat.

    abstract::We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ni Y,Su M,Qiu Y,Chen M,Liu Y,Zhao A,Jia W

    更新日期:2007-02-20 00:00:00

  • Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.

    abstract::The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

    更新日期:2007-04-03 00:00:00

  • In vivo glycosylation suppresses the aggregation of amyloidogenic hen egg white lysozymes expressed in yeast.

    abstract::The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Song Y,Azakami H,Hamasu M,Kato A

    更新日期:2001-02-23 00:00:00

  • Ca2+-dependent K+ channels from rat olfactory cilia characterized in planar lipid bilayers.

    abstract::Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Castillo K,Bacigalupo J,Wolff D

    更新日期:2005-03-14 00:00:00

  • Up- and downregulated genes in muscles that undergo developmentally programmed cell death in the insect Manduca sexta.

    abstract::This study was designed to investigate changes in gene expression associated with stage-specific programmed cell death (PCD) in intersegmental muscles (ISMs) of the moth, Manduca sexta. The technique of differential display reverse transcription PCR was applied to compare mRNA levels before and after the onset of PCD ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Löw P,Talián GC,Sass M

    更新日期:2005-09-12 00:00:00

  • Heme a induces assembly of rat liver cytochrome c oxidase subunits I--III in isolated mitochondria.

    abstract::The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wielburski A,Nelson BD

    更新日期:1984-11-19 00:00:00

  • TAK1 mediates an activation signal from toll-like receptor(s) to nuclear factor-kappaB in lipopolysaccharide-stimulated macrophages.

    abstract::Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Irie T,Muta T,Takeshige K

    更新日期:2000-02-11 00:00:00

  • Vitamin K-dependent carboxylase: synthesis of an inhibitor of the glutamyl binding site.

    abstract::Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

    更新日期:1983-02-07 00:00:00

  • A novel dimer of a C-type lectin-like heterodimer from the venom of Calloselasma rhodostoma (Malayan pit viper).

    abstract::We have isolated a potent platelet aggregation inducer from the crude venom of Calloselasma rhodostoma (Malayan pit viper), termed rhodoaggretin, with a novel oligomeric structure consisting of a dimer of C-type lectin-like heterodimers. On the basis of its native molecular mass of 66 kDa, and a M(r) of 30 kDa for its...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang R,Kong C,Kolatkar P,Chung MC

    更新日期:2001-11-23 00:00:00

  • Antithrombin Glasgow, 393 Arg to His: a P1 reactive site variant with increased heparin affinity but no thrombin inhibitory activity.

    abstract::Antithrombin Glasgow is a hereditary abnormal antithrombin that has lost thrombin inhibitory activity. It was isolated from the plasma of a 41-year-old male with a history of thrombotic events. Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Owen MC,Beresford CH,Carrell RW

    更新日期:1988-04-25 00:00:00

  • Homing markers for atherosclerosis: applications for drug delivery, gene delivery and vascular imaging.

    abstract::Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Houston P,Goodman J,Lewis A,Campbell CJ,Braddock M

    更新日期:2001-03-09 00:00:00

  • Substrate interactions in cytochrome P-450: correlation between carbon-13 nuclear magnetic resonance chemical shifts and C-O vibrational frequencies.

    abstract::13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

    更新日期:1995-05-08 00:00:00

  • Genomic exploration of the hemiascomycetous yeasts: 15. Pichia sorbitophila.

    abstract::This paper reports the genomic analysis of the strain CBS7064 of Pichia sorbitophila, a homothallic diploid yeast. We sequenced 4829 random sequence tags of about 1 kb and compared them to the Saccharomyces cerevisiae gene products. Approximately 1300 nuclear genes, 22 tRNAs, the rDNA locus, and six mitochondrial gene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: de Montigny J,Spehner C,Souciet J,Tekaia F,Dujon B,Wincker P,Artiguenave F,Potier S

    更新日期:2000-12-22 00:00:00

  • Complete amino acid sequence of fetal bovine serum acetylcholinesterase and its comparison in various regions with other cholinesterases.

    abstract::The complete amino acid sequence of a mammalian acetylcholinesterase from fetal bovine serum (FBS AChE) is presented. This enzyme has a high degree of sequence identity with other cholinesterases, liver carboxyesterases, esterase-6, lysophospholipase, and thyroglobulin. The locations of 191 amino acids in 10 regions o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Doctor BP,Chapman TC,Christner CE,Deal CD,De La Hoz DM,Gentry MK,Ogert RA,Rush RS,Smyth KK,Wolfe AD

    更新日期:1990-06-18 00:00:00

  • Is the acetylcholine releasing protein mediatophore present in rat brain?

    abstract::Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Israël M,Lesbats B,Morel N,Manaranche R,Le Gal la Salle G

    更新日期:1988-06-20 00:00:00

  • A genomic perspective on human proteases.

    abstract::Over 400 human proteases documented in secondary databases can already be delineated in genomic sequence. A Genome Ontology annotation of 30585 sequences in the provisional human proteome set recognises 498 proteases, i.e. 1.6%. Homology searches against finished sequence and comparisons between mouse and zebrafish ar...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Southan C

    更新日期:2001-06-08 00:00:00

  • Metabolic activation of 1,2-dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes.

    abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tomasi A,Albano E,Dianzani MU,Slater TF,Vannini V

    更新日期:1983-08-22 00:00:00

  • A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells.

    abstract::A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

    更新日期:1990-07-30 00:00:00

  • Biosynthesis and turnover of anandamide and other N-acylethanolamines in peritoneal macrophages.

    abstract::Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kuwae T,Shiota Y,Schmid PC,Krebsbach R,Schmid HH

    更新日期:1999-10-01 00:00:00

  • A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP).

    abstract::Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikawa M,Kominami K,Yoshimura Y,Tanaka K,Nishimune Y,Okabe M

    更新日期:1995-11-13 00:00:00

  • In vitro reconstitution of messenger ribonucleoprotein particles from globin messenger RNA and cytosol proteins.

    abstract::Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gaedigk R,Oehler S,Köhler K,Setyono B

    更新日期:1985-01-07 00:00:00

  • Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation.

    abstract::Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Demoulin JB,Grasso L,Atkins JM,Stevens M,Louahed J,Levitt RC,Nicolaides NC,Renauld JC

    更新日期:2000-10-06 00:00:00

  • Multisite phosphorylation of the glycogen-binding subunit of protein phosphatase-1G by cyclic AMP-dependent protein kinase and glycogen synthase kinase-3.

    abstract::The glycogen-binding (G) subunit of protein phosphatase-1G is phosphorylated stoichiometrically by glycogen synthase kinase-3 (GSK3), and with a greater catalytic efficiency than glycogen synthase, but only after prior phosphorylation by cyclic AMP-dependent protein kinase (A-kinase) at site 1. The residues phosphoryl...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Dent P,Campbell DG,Hubbard MJ,Cohen P

    更新日期:1989-05-08 00:00:00

  • Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis.

    abstract::Nine of the cbi genes from the 17.5 kb cob operon of Salmonella typhimurium previously shown by genetic studies to be involved in the biosynthesis of cobinamide from precorrin-2, have been subcloned and expressed in Escherichia coli. Seven of the gene products were found in the soluble fraction of cell lysates and hav...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roessner CA,Warren MJ,Santander PJ,Atshaves BP,Ozaki S,Stolowich NJ,Iida K,Scott AI

    更新日期:1992-04-13 00:00:00

  • Efficient CD4 binding and immunosuppressive properties of the 13B8.2 monoclonal antibody are displayed by its CDR-H1-derived peptide CB1.

    abstract::A systematic exploration of the V(H)2/V(kappa)12-13 variable domains of the anti-CD4 monoclonal antibody (mAb) 13B8.2 was performed by the Spot method to screen for paratope-derived peptides (PDPs) demonstrating CD4 binding ability. Nine peptides, named CB1 to CB9, were identified, synthesized in a cyclic and soluble ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bès C,Briant-Longuet L,Cerruti M,De Berardinis P,Devauchelle G,Devaux C,Granier C,Chardès T

    更新日期:2001-11-09 00:00:00

  • Ground state isomerization of a model green fluorescent protein chromophore.

    abstract::The relationship between ground state cis-trans isomerization and protonation state is explored for a model green fluorescent protein chromophore, 4-hydroxybenzylidene-1,2-dimethylimidazolinone (HBDI). We find that the protonation state has only a modest effect on the free energy differences between cis and trans isom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: He X,Bell AF,Tonge PJ

    更新日期:2003-08-14 00:00:00