Abstract:
:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a much higher elevation in intracellular calcium. This elevation together with [3H]AA release were markedly reduced by EDTA. However, addition of ionophore A23187 enhanced the release of [3H]AA by gamma-thrombin to the levels similar to those of alpha-thrombin. We conclude that gamma-thrombin is able to activate PLA2 and suggest that calcium influx may be a limiting factor for this activation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Touqui L,Jandrot-Perrus M,Vargaftig BBdoi
10.1016/0014-5793(89)81142-1subject
Has Abstractpub_date
1989-09-25 00:00:00pages
445-50issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81142-1journal_volume
255pub_type
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