Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence.

abstract：:Tissue factor pathway inhibitor (TFPI) inhibits the activity of coagulation factors VIIa and Xa through Kunitz domains, thereby inhibiting the activity of tissue factor. However, it has been shown that the C-terminal of this inhibitor is essential for the maximal anticoagulant activity of TFPI. We have investigated th...

journal_title：FEBS letters

authors： Ettelaie C,Adam JM,James NJ,Oke AO,Harrison JA,Bunce TD,Bruckdorfer KR

更新日期：1999-12-17 00:00:00

abstract：:Free radicals, including dopamine (DA)-oxidized metabolites, have long been implicated in pathogenesis of Parkinson's disease (PD). However, the relationships between such oxidative stresses and alpha-synuclein (alpha-S), a major constituent of Lewy bodies, remain unknown. In this study, we established neuronal cells ...

journal_title：FEBS letters

authors： Hasegawa T,Matsuzaki-Kobayashi M,Takeda A,Sugeno N,Kikuchi A,Furukawa K,Perry G,Smith MA,Itoyama Y

更新日期：2006-04-03 00:00:00

abstract：:The mongoose AChR alpha-subunit has been cloned and shown to be highly homologous to other AChR alpha-subunits, with only six differences in amino acid residues at positions that are conserved in animal species that bind alpha-bungarotoxin (alpha-BTX). Four of these six substitutions cluster in the ligand binding site...

journal_title：FEBS letters

authors： Asher O,Jensen BS,Lupu-Meiri M,Oron Y,Fuchs S

更新日期：1998-04-17 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

journal_title：FEBS letters

authors： Koteliansky VE,Gneushev GN,Belkin AM

更新日期：1985-03-11 00:00:00

abstract：:The present study was designed to investigate whether and how the purinergic stimulation of rat ventricular myocytes modulates the cAMP-dependent pathway. Stimulation of cardiomyocytes with ATPgammaS in the presence of the phosphodiesterase inhibitor IBMX increases by 3-fold intracellular cAMP content. In contrast to ...

journal_title：FEBS letters

authors： Pucéat M,Bony C,Jaconi M,Vassort G

更新日期：1998-07-17 00:00:00

abstract：:By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

journal_title：FEBS letters

authors： Kambe T,Murakami M,Kudo I

更新日期：1999-06-18 00:00:00

abstract：:Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function rel...

journal_title：FEBS letters

authors： Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia J

更新日期：1996-12-16 00:00:00

abstract：:Human parainfluenza virus type 3 (hPIV3) recognizes both α2,3- and α2,6-linked sialic acids, whereas human parainfluenza virus type 1 (hPIV1) recognizes only α2,3-linked sialic acids. To identify amino acid residues that confer α2,6-linked sialic acid recognition of hPIV3, amino acid residues in or neighboring the sia...

journal_title：FEBS letters

authors： Fukushima K,Takahashi T,Ueyama H,Takaguchi M,Ito S,Oishi K,Minami A,Ishitsubo E,Tokiwa H,Takimoto T,Suzuki T

更新日期：2015-05-08 00:00:00

abstract：:The contact-dependent intrinsic pathway of fibrinolysis involving factor XII, prekallikrein (PK) and pro-urokinase (pro-UK) remains poorly understood. Casein autography of washed, intact platelets revealed both PK and pro-UK. Accordingly, platelets may mediate physiological thrombolysis by this pathway since factor XI...

journal_title：FEBS letters

authors： Gurewich V,Johnstone M,Loza JP,Pannell R

更新日期：1993-03-08 00:00:00

abstract：:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...

journal_title：FEBS letters

authors： Kitagawa Y,Murakami A,Sugimoto E

更新日期：1984-01-02 00:00:00

abstract：:Many proteins located in the intermembrane space (IMS) of mitochondria are characterized by a low molecular mass, contain highly conserved cysteine residues and coordinate metal ions. Studies on one of these proteins, Tim13, revealed that net translocation across the outer membrane is driven by metal-dependent folding...

journal_title：FEBS letters

authors： Terziyska N,Lutz T,Kozany C,Mokranjac D,Mesecke N,Neupert W,Herrmann JM,Hell K

更新日期：2005-01-03 00:00:00

abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...

journal_title：FEBS letters

authors： Ledent P,Duez C,Vanhove M,Lejeune A,Fonzé E,Charlier P,Rhazi-Filali F,Thamm I,Guillaume G,Samyn B,Devreese B,Van Beeumen J,Lamotte-Brasseur J,Frère JM

更新日期：1997-08-18 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

journal_title：FEBS letters

authors： Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

更新日期：1990-06-04 00:00:00

abstract：:The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

journal_title：FEBS letters

authors： Oertle M,Winterhalter KH,Di Iorio EE

更新日期：1983-03-07 00:00:00

abstract：:Astrocytes (AC) induce blood-brain barrier (BBB) properties in brain endothelial cells (EC). As antioxidative activity (AOA) is assumed to be a BBB characteristic, we tested whether AC improve AOA of EC. Monocultivated AC showed higher AOA [manganese superoxide dismutase (SOD), catalase (Cat), glutathione peroxidase (...

journal_title：FEBS letters

authors： Schroeter ML,Mertsch K,Giese H,Müller S,Sporbert A,Hickel B,Blasig IE

更新日期：1999-04-23 00:00:00

abstract：:The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ter...

journal_title：FEBS letters

authors： Mercaldi GF,Dawson A,Hunter WN,Cordeiro AT

更新日期：2016-08-01 00:00:00

abstract：:While many antimicrobial peptides (AMPs) disrupt bacterial membranes, some translocate into bacteria and interfere with intracellular processes. Buforin II and DesHDAP1 are thought to kill bacteria by interacting with nucleic acids. Here, molecular modeling and experimental measurements are used to show that neither n...

journal_title：FEBS letters

authors： Sim S,Wang P,Beyer BN,Cutrona KJ,Radhakrishnan ML,Elmore DE

更新日期：2017-03-01 00:00:00

abstract：:T cells are stimulated by the engagement of antigen, cytokine, pathogen, and hormone receptors. While research performed over many years has focused on deciphering the molecular components of these pathways, recent data underscore the importance of the metabolic environment in conditioning responses to receptor engage...

journal_title：FEBS letters

authors： Yong CS,Abba Moussa D,Cretenet G,Kinet S,Dardalhon V,Taylor N

更新日期：2017-10-01 00:00:00

abstract：:X-linked inhibitor of apoptosis protein (XIAP) is a potent antagonist of caspases, and functions as a ubiquitin-E3 ligase by itself and for caspases. Recently, NEDD8, a ubiquitin-like modifier, has been suggested to be used for modification of caspase-7 mediated by XIAP. However, it is not clear whether caspase-7 is a...

journal_title：FEBS letters

authors： Nagano T,Hashimoto T,Nakashima A,Kikkawa U,Kamada S

更新日期：2012-06-04 00:00:00

abstract：:Michaelis-Menten kinetics of glycerol-3-phosphate dehydrogenase activity in proteoliposomes from brown adipose tissue mitochondria with exogenously added phospholipids or cholesterol was measured. It was shown that changes in membrane lipid composition affected the membrane lateral pressure and therefore modulated the...

journal_title：FEBS letters

authors： Amler E,Jasińska R,Drahota Z,Zborowski J

更新日期：1990-10-01 00:00:00

abstract：:A new inositol derivative could be isolated from the Archaeum Pyrococcus woesei and identified as di-myo-inositol-1,1'-phosphate by 1H, 31P NMR spectroscopy, mass spectrometry and thin layer chromatography. In P. woesei, this inositol phosphate represents the dominant counterion of K+ which ranges from 500 to 600 mM. ...

journal_title：FEBS letters

authors： Scholz S,Sonnenbichler J,Schäfer W,Hensel R

更新日期：1992-07-20 00:00:00

abstract：:The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C(4)=C(5) and C(5)-C(6) bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-R(A), but ...

journal_title：FEBS letters

authors： Seibeck S,Borucki B,Otto H,Inomata K,Khawn H,Kinoshita H,Michael N,Lamparter T,Heyn MP

更新日期：2007-11-27 00:00:00

abstract：:Using the polymerase chain reaction (PCR), we identified a gene family including more than 60 members which encoded similar G protein-coupled seven-transmembrane receptors. Sequence analyses of six representatives out of the 60 PCR clones showed that they had significant structural similarity to olfactory and optic re...

journal_title：FEBS letters

authors： Abe K,Kusakabe Y,Tanemura K,Emori Y,Arai S

更新日期：1993-02-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00

abstract：:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently,...

journal_title：FEBS letters

authors： Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt W

更新日期：2015-07-08 00:00:00

abstract：:Hypermethylated in cancer, a new candidate tumor suppressor gene located in 17p13.3, encodes a protein with five Krüppel-like C2H2 zinc finger motifs and a N-terminal protein/protein interaction domain called broad complex, tramtrack and bric à brac/poxviruses and zinc finger domain. Hypermethylated in cancer appears ...

journal_title：FEBS letters

authors： Guerardel C,Deltour S,Leprince D

更新日期：1999-05-28 00:00:00

abstract：:The enantiomers of U50,488, ligands highly selective for kappa-opioid receptors, have been prepared by a refined procedure and their optical purity demonstrated. The absolute configuration of (+)-trans-2-pyrrolidinyl-N-methylcyclohexylamine, a chemically versatile intermediate for synthesis of analogs of kappa-opioid ...

journal_title：FEBS letters

authors： DeCosta B,George C,Rothman RB,Jacobson AE,Rice KC

更新日期：1987-11-02 00:00:00

abstract：:The purinergic P2X(7) receptor is an ATP-receptor channel predominantly expressed in immune cells. P2X(7) has been cloned from human, rat and mouse. Here we report cloning of the Xenopus laevis P2X(7) receptor (xP2X(7)). xP2X(7) is only about 50% identical to the mammalian homologues, shows a broad tissue expression p...

journal_title：FEBS letters

authors： Paukert M,Hidayat S,Gründer S

更新日期：2002-02-27 00:00:00