The mongoose acetylcholine receptor alpha-subunit: analysis of glycosylation and alpha-bungarotoxin binding.

abstract：:Anthrax is a severe bacterial infection that occurs when Bacillus anthracis spores gain access into the body and germinate in macrophages, causing septicemia and toxemia. Anthrax toxin is a binary A-B toxin composed of protective antigen (PA), lethal factor (LF), and edema factor (EF). PA mediates the entry of either ...

journal_title：FEBS letters

authors： Ascenzi P,Visca P,Ippolito G,Spallarossa A,Bolognesi M,Montecucco C

更新日期：2002-11-20 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:The cytosolic peptidyl-prolyl cis/trans isomerase cyclophilin from pig kidney can accelerate catalytically the cis/trans isomerization of prolyl peptide bonds. One- and two-dimensional 1H NMR spectroscopy was used to prove that the polypeptide hormone calcitonin is a substrate for cyclophilin. Isomerization of only on...

journal_title：FEBS letters

authors： Kern D,Drakenberg T,Wikström M,Forsén S,Bang H,Fischer G

更新日期：1993-06-01 00:00:00

abstract：:In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

journal_title：FEBS letters

authors： Seebahn A,Rose M,Enz R

更新日期：2008-07-09 00:00:00

abstract：:The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

journal_title：FEBS letters

authors： Hobohm U,Sander C

更新日期：1993-11-01 00:00:00

abstract：:The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...

journal_title：FEBS letters

authors： Berman HM,Coimbatore Narayanan B,Di Costanzo L,Dutta S,Ghosh S,Hudson BP,Lawson CL,Peisach E,Prlić A,Rose PW,Shao C,Yang H,Young J,Zardecki C

更新日期：2013-04-17 00:00:00

abstract：:The macromolecular diversity and concentrations in the fluid phase of cytoplasm constitute conditions necessary and sufficient for aqueous phase separation. Consequences of phase separation in cytoplasm, including its 'compartmentation', are inferred from analogies with the physicochemical properties of aqueous two-ph...

journal_title：FEBS letters

authors： Walter H,Brooks DE

更新日期：1995-03-20 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:Amino acid sequences coded for by 13 different genes of mammalian mitochondrial DNA (mtDNA) including 8 unassigned open reading frames (URFs) were compared in pairs. It was found that significant homologies exist among the amino acid sequences of the three URFs (URF2, URF4 and URF5) with a probability of occurrence of...

journal_title：FEBS letters

authors： Kikuno R,Miyata T

更新日期：1985-09-09 00:00:00

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00

abstract：:To clarify the function of osteopontin in osteoblast differentiation, we have examined the signal transduction pathway in an osteoblastic cell line (UMR106-6) bound to osteopontin, fibronectin, vitronectin and collagen type I surfaces. This was done by investigating the production and autophosphorylation of focal adhe...

journal_title：FEBS letters

authors： Liu YK,Uemura T,Nemoto A,Yabe T,Fujii N,Ushida T,Tateishi T

更新日期：1997-12-22 00:00:00

abstract：:Culturing human fibroblasts in a three-dimensional collagen matrix leads to a reduction of collagen I by more than 90%, both on the level of mRNA steady-state as well as protein. In order to differentiate changes in de novo transcription and posttranscriptional control, nuclear run on assays and pulse/chase experiment...

journal_title：FEBS letters

authors： Eckes B,Mauch C,Hüppe G,Krieg T

更新日期：1993-03-01 00:00:00

abstract：:Mice are widely used for exploring obesity physiology and treatment. However, thermal biology is different between small and large mammals. In this Review, we discuss how the effect of environmental temperature must be understood to ensure applicability of mouse experiments to human obesity. At ambient environmental t...

journal_title：FEBS letters

authors： Reitman ML

更新日期：2018-06-01 00:00:00

abstract：:Nitric oxide (.NO) synthase (NOS) activity in subcellular fractions from cultured endothelial cells (EC) and lipopolysaccharide-activated J774.2 monocyte/macrophages was investigated by monitoring the .NO-mediated increase in intracellular cyclic GMP in LLC-PK1 pig kidney epithelial cells. The constitutive NOS in EC (...

journal_title：FEBS letters

authors： Hecker M,Walsh DT,Vane JR

更新日期：1991-12-09 00:00:00

abstract：:DNA polymerase alpha-primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C-terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the int...

journal_title：FEBS letters

authors： Smith RW,Nasheuer HP

更新日期：2002-09-11 00:00:00

abstract：:Farnesoic acid is a signaling molecule that inhibits the transition from budding yeast to filament formation in Candida albicans, but the molecular mechanism regulated by this substance is unknown. In this study, we analyzed the function of CaPHO81, which is induced by farnesoic acid. The pho81Δ mutant cells existed e...

journal_title：FEBS letters

authors： Chung SC,Kim TI,Ahn CH,Shin J,Oh KB

更新日期：2010-11-19 00:00:00

abstract：:We previously found a brain-specific glycoprotein in the rat brain. It postnatally increases and is rich in the mature brain. We cloned cDNA of this protein. It is composed of a signal peptide, a V-type immunoglobulin domain, two C1-type immunoglobulin domains, a transmembrane segment and a cytoplasmic region containi...

journal_title：FEBS letters

authors： Sano S,Ohnishi H,Omori A,Hasegawa J,Kubota M

更新日期：1997-07-14 00:00:00

abstract：:Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes ER stress. The ER stress sensor inositol requiring enzyme-1beta (IRE1β), which is specifically expressed in intestinal epithelial cells, is thought to be involved in translational repression. However, its mechanism of action is not fully unders...

journal_title：FEBS letters

authors： Nakamura D,Tsuru A,Ikegami K,Imagawa Y,Fujimoto N,Kohno K

更新日期：2011-01-03 00:00:00

abstract：:The mutation of a single leucine residue (L38) to methionine (M) is known experimentally to significantly increase the affinity of the synthetic four-alpha-helix bundle (Aalpha(2))(2) for the anesthetic halothane. We present a molecular dynamics study of the mutant (Aalpha(2)-L38M)(2) peptide, which consists of a dime...

journal_title：FEBS letters

authors： Davies LA,Zhong Q,Klein ML,Scharf D

更新日期：2000-07-28 00:00:00

abstract：:Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...

journal_title：FEBS letters

authors： Oe S,Yoneda Y

更新日期：2010-08-04 00:00:00

abstract：:Muscarinic K+ channels (IK(ACh)) in native atrial myocytes are activated by betagamma subunits of pertussis toxin (Ptx)-sensitive heterotrimeric G proteins coupled to different receptors. betagamma subunits of Ptx-insensitive Gs, coupled to beta-adrenergic receptors, do not activate native IK(ACh). In atrial myocytes ...

journal_title：FEBS letters

authors： Bender K,Wellner-Kienitz MC,Meyer T,Pott L

更新日期：1998-11-13 00:00:00

abstract：:The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...

journal_title：FEBS letters

authors： Rodríguez-Torres AM,Lamas-Maceiras M,García-Díaz R,Freire-Picos MA

更新日期：2013-08-19 00:00:00

abstract：:To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

journal_title：FEBS letters

authors： Tomasselli AG,Frank R,Schiltz E

更新日期：1986-07-07 00:00:00

abstract：:The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

journal_title：FEBS letters

authors： Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

更新日期：1998-06-12 00:00:00

abstract：:Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

journal_title：FEBS letters

authors： Zifarelli G,Pusch M

更新日期：2010-05-17 00:00:00

abstract：:Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...

journal_title：FEBS letters

authors： Colston JT,de la Rosa SD,Strader JR,Anderson MA,Freeman GL

更新日期：2005-04-25 00:00:00

abstract：:The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...

journal_title：FEBS letters

authors： Miteva M,Andersson M,Karshikoff A,Otting G

更新日期：1999-11-26 00:00:00

abstract：:The geometries are reported for interacting arginine-carboxyl pairs obtained from 37 high resolution protein structures solved to a resolution of 2.0 A or better. The closest interatomic distance between the guanidinium and carboxyl is less than 4.2 A for 74 arginine and carboxyl groups, with the majority of these lyi...

journal_title：FEBS letters

authors： Singh J,Thornton JM,Snarey M,Campbell SF

更新日期：1987-11-16 00:00:00

abstract：:Pathway analysis methods are frequently applied to cancer gene expression data to identify dysregulated pathways. These methods often infer pathway activity based on the expression of genes belonging to a given pathway, even though the proteins ultimately determine the activity of a given pathway. Furthermore, the ass...

journal_title：FEBS letters

authors： Szalai B,Saez-Rodriguez J

更新日期：2020-12-01 00:00:00

abstract：:G protein-coupled receptor kinase 2 (GRK2) plays a key role in determining the rate and extent of G protein-coupled receptor (GPCR) desensitization and resensitization. Recent data indicate that GRK2 activity, subcellular distribution and expression are tightly regulated. The important physiological function of GRK2 a...

journal_title：FEBS letters

authors： Aragay AM,Ruiz-Gómez A,Penela P,Sarnago S,Elorza A,Jiménez-Sainz MC,Mayor F Jr

更新日期：1998-06-23 00:00:00