Abstract:
:G protein-coupled receptor kinase 2 (GRK2) plays a key role in determining the rate and extent of G protein-coupled receptor (GPCR) desensitization and resensitization. Recent data indicate that GRK2 activity, subcellular distribution and expression are tightly regulated. The important physiological function of GRK2 as a modulator of the efficacy of GPCR signal transduction systems is exemplified by its relevance in cardiovascular physiopathology as well as by its emerging role in the regulation of chemokine receptors.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Aragay AM,Ruiz-Gómez A,Penela P,Sarnago S,Elorza A,Jiménez-Sainz MC,Mayor F Jrdoi
10.1016/s0014-5793(98)00495-5subject
Has Abstractpub_date
1998-06-23 00:00:00pages
37-40issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579398004955journal_volume
430pub_type
杂志文章,评审相关文献
FEBS LETTERS文献大全abstract::S100, a subfamily of the EF-hand type calcium sensing proteins, is implicated in many cellular functions including muscle contractility. Two isoforms, S100A1 and S100B, at 2-10 microM significantly inhibit active tension, stiffness and ATPase of skinned single rabbit psoas muscle fibers at sub-maximal (pCa approximate...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02444-9
更新日期:2001-05-25 00:00:00
abstract::The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.036
更新日期:2007-02-06 00:00:00
abstract::A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80189-8
更新日期:1987-09-28 00:00:00
abstract::Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.04.040
更新日期:2013-06-19 00:00:00
abstract::We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01004-1
更新日期:1997-09-08 00:00:00
abstract::Platinum electrodes can be coated with cytochrome b5-phospholipid monolayers by the Langmuir-Blodgett technique. Cyclic voltammetry of a series of dyes shows that the coated electrodes become selective for certain electroactive species. The electron transfer reactions of negatively charged species are inhibited at the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81463-1
更新日期:1987-03-09 00:00:00
abstract::Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01125-x
更新日期:1995-10-30 00:00:00
abstract::2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81395-2
更新日期:1984-05-07 00:00:00
abstract::Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.02.038
更新日期:2011-05-20 00:00:00
abstract::The O2-dependent formation of side products during the oxidation of veratryl alcohol (VA) by lignin peroxidase has previously been proposed to start with the attack of H2O on the VA radical cation (VA*+). This initial reaction is unlikely since it would also lead to side product formation in the absence of O2, which i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02379-6
更新日期:2001-01-05 00:00:00
abstract::The protein kinase C activator, phorbol-12-myristate-13-acetate (PMA), augments the cyclic AMP accumulation induced by forskolin in pheochromocytoma (PC 12) cells with an EC50 value of 14 nM, while having no effect on basal values. At a concentration of 100 nM PMA markedly augmented the magnitude of the forskolin resp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80227-7
更新日期:1986-02-03 00:00:00
abstract::Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00178-4
更新日期:2004-03-26 00:00:00
abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.004
更新日期:2007-06-12 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the leading cause of lower respiratory tract disease in infants. The HRSV small hydrophobic (SH) protein plays an important role in HRSV pathogenesis, although its mode of action is unclear. Analysis of the ability of SH protein to induce membrane permeability and form homo-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.006
更新日期:2010-07-02 00:00:00
abstract::Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00916-3
更新日期:1994-09-19 00:00:00
abstract::The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80931-1
更新日期:1988-11-07 00:00:00
abstract::The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80137-1
更新日期:1989-01-30 00:00:00
abstract::Stresses that impair the function of the endoplasmic reticulum (ER) lead to an accumulation of unfolded protein in the ER. Under these conditions, the expression of a variety of genes involved in preventing the accumulation of the unfolded proteins is induced. Yeast Hrd1p is an ER stress-inducible ER membrane protein ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03660-8
更新日期:2002-12-04 00:00:00
abstract::The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.054
更新日期:2004-06-04 00:00:00
abstract::Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/S0014-5793(04)00209-1
更新日期:2004-04-30 00:00:00
abstract::The structure of rabbit liver Hsp90 was reevaluated by limited trypsinolysis, N-terminal sequencing and determination of the site that is phosphorylated by casein kinase II. Limited proteolysis results in formation of four groups of large peptides with M(r) in the range of 26-41 kDa. Peptides with M(r) 39-41 kDa were ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01056-x
更新日期:1999-09-03 00:00:00
abstract::Casein kinase Ialpha (CKIalpha) belongs to a family of serine/threonine protein kinases involved in membrane trafficking, RNA processing, mitotic spindle formation and cell cycle progression. In this report, we identified several CKIalpha interacting proteins including RCC1, high mobility group proteins 1 and 2 (HMG1,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02614-5
更新日期:2002-04-24 00:00:00
abstract::Using a cDNA library from bovine adrenal medulla, and, subsequently, a bovine genomic library, we have isolated the gene coding for a non inactivating potassium channel. This gene encodes a 597-amino acid protein which we have called BAK5 as its sequence is very similar to members of Kv1.5 potassium channel family. Ne...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01117-6
更新日期:1994-11-07 00:00:00
abstract::Despite significant increases in circulating concentrations of lipid fuels (triacylglycerol, non-esterified fatty acids (NEFA) and ketone bodies) in late-pregnant rats sampled in the fed (absorptive) state, cardiac and skeletal muscle active pyruvate dehydrogenase (PDHa) activities remained comparable with those obser...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80091-8
更新日期:1993-04-26 00:00:00
abstract::To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81474-3
更新日期:1990-11-26 00:00:00
abstract::Granulocytes from human eosinophilic donors were incubated with arachidonic acid or 15-hydroxyeicosatetraenoic acid (15-HETE) and stimulated with the ionophore A23187. The eicosanoids were extracted with reversed-phase cartridges and subjected to RP-HPLC analysis. When extracts from eosinophil-enriched populations wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81078-6
更新日期:1989-09-11 00:00:00
abstract::The requirement of divalent cations for binding of the 46 kDa mannose 6-phosphate receptors to phosphomannan and pentamannose 6-phosphate-substituted bovine serum albumin was examined. Receptors from human liver and human brain bound to both affinity ligands in the absence or presence of divalent cations with similar ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80176-5
更新日期:1988-09-12 00:00:00
abstract::Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00636-9
更新日期:1994-07-25 00:00:00
abstract::The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03501-9
更新日期:2002-11-06 00:00:00
abstract::In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81388-2
更新日期:1989-04-24 00:00:00