Specific activation of adenylyl cyclase V by a purinergic agonist.

abstract：:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

journal_title：FEBS letters

authors： Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

更新日期：1989-04-10 00:00:00

abstract：:Isolated rat islets of Langerhans permeabilised by high-voltage discharge secreted insulin in response to elevations in Ca2+ over the range 100 nM to 10 microM Ca2+. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), had no effects on insulin secretion in the absence of Ca2+. In the presence of Ca2+ concen...

journal_title：FEBS letters

authors： Jones PM,Stutchfield J,Howell SL

更新日期：1985-10-21 00:00:00

abstract：:The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact w...

journal_title：FEBS letters

authors： Bier M,Fath S,Tschochner H

更新日期：2004-04-23 00:00:00

abstract：:The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...

journal_title：FEBS letters

authors： Dornmair K

更新日期：1988-08-01 00:00:00

abstract：:Apart from the 8 kDa subunit, which is of chloroplast origin, most of the small polypeptides of the PS I reaction center from higher plants are encoded in nuclear genes. We describe here the first nucleotide sequence of a nuclear component of this photosystem, the precursor of the 20 kDa protein. The deduced sequence ...

journal_title：FEBS letters

authors： Lagoutte B

更新日期：1988-05-23 00:00:00

abstract：:It has been hypothesized that hyperthermia promotes oxygen-centered free radical formation in cells; however, to date there is no direct evidence of this heat-induced increase in oxygen free radical flux. Using electron paramagnetic resonance (EPR) spin trapping, we sought direct evidence for free radical generation d...

journal_title：FEBS letters

authors： Flanagan SW,Moseley PL,Buettner GR

更新日期：1998-07-17 00:00:00

abstract：:In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by inte...

journal_title：FEBS letters

authors： Pedraza CE,Baltrons MA,García A

更新日期：2001-11-02 00:00:00

abstract：:Hepatitis C virus (HCV) often establishes a persistent infection that leads to chronic liver diseases. The viral core protein modulates various cellular activities involved in this process. We found two mutations, K23E and V31A, in the core gene of the transfected HCV JFH-1 genome, which had been replicated for a prol...

journal_title：FEBS letters

authors： Kang JI,Kwon YC,Ahn BY

更新日期：2012-05-07 00:00:00

abstract：:Cardiac toxicity is an uncommon but potentially serious complication of cancer therapy, especially with anthracyclines. One of the most effective anticancer drugs is doxorubicin, but its value is limited by the risk of developing cardiomyopathy and ventricular arrhythmia. When applied to a network of periodically cont...

journal_title：FEBS letters

authors： Gorelik J,Vodyanoy I,Shevchuk AI,Diakonov IA,Lab MJ,Korchev YE

更新日期：2003-07-31 00:00:00

abstract：:Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

journal_title：FEBS letters

authors： Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

更新日期：2012-01-20 00:00:00

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00

abstract：:The toxicity of the beta-amyloid (Abeta) peptide of Alzheimer's disease may relate to its polymerisation state (i.e. fibril content). We have shown previously that plasma lipoproteins, particularly when oxidised, greatly enhance Abeta polymerisation. In the present study the nature of the interactions between both nat...

journal_title：FEBS letters

authors： Stanyer L,Betteridge DJ,Smith CC

更新日期：2002-05-08 00:00:00

abstract：:The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecu...

journal_title：FEBS letters

authors： De Rossi MC,De Rossi ME,Sued M,Rodríguez D,Bruno L,Levi V

更新日期：2015-09-14 00:00:00

abstract：:HERC proteins are characterized by having one or more RCC1-like domains as well as a C-terminal HECT domain in their amino acid sequences. This has led researchers to suggest that they may act as both guanine nucleotide exchange factors and E3 ubiquitin ligases. Here we describe a physical interaction between the HECT...

journal_title：FEBS letters

authors： Garcia-Gonzalo FR,Cruz C,Muñoz P,Mazurek S,Eigenbrodt E,Ventura F,Bartrons R,Rosa JL

更新日期：2003-03-27 00:00:00

abstract：:Microdissected, beta-cell-rich pancreatic islets from ob/ob mice were used in studies of 86Rb+ transport. D-Glucose (20 mM) induced a biphasic reduction in 86Rb+ efflux. The reduction stabilized within 10 min at 34% of the efflux rate at zero glucose. The initial 86Rb+ uptake (5 min) was dose-dependently reduced by ou...

journal_title：FEBS letters

authors： Lindström P,Norlund L,Sehlin J

更新日期：1986-05-05 00:00:00

abstract：:To investigate the cis-acting DNA elements that are involved in the regulation of class I major histocompatibility complex genes by interferon, several promoter fragments of the H-2Kk gene were linked to the reporter chloramphenicol acetyl transferase (CAT) gene, and the CAT expression was analyzed in stable transfect...

journal_title：FEBS letters

authors： Pascucci A,Pannuti A,La Mantia G,Lania L

更新日期：1988-01-04 00:00:00

abstract：:We demonstrated that unmodified and modified (phosphorothioate) oligonucleotides prevent cDNA synthesis by AMV or HIV reverse transcriptases. Antisense oligonucleotide/RNA hybrids specifically arrest primer extension. The blockage involves the degradation of the RNA fragment bound to the antisense oligonucleotide by t...

journal_title：FEBS letters

authors： Hatta T,Kim SG,Nakashima H,Yamamoto N,Sakamoto K,Yokoyama S,Takaku H

更新日期：1993-09-13 00:00:00

abstract：:Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...

journal_title：FEBS letters

authors： Bathurst IC,Errington DM,Foreman RC,Judah JD,Carrell RW

更新日期：1985-04-22 00:00:00

abstract：:The cellular uptake of a model antisense oligonucleotide complementary to 21 bases of the bovine GLUT-1 glucose transporter mRNA and a model vasopressin peptide that were biotinylated, was markedly stimulated by the presence of avidin, a cationic protein. Conversely, the bacteria homologue of avidin, streptavidin, whi...

journal_title：FEBS letters

authors： Pardridge WM,Boado RJ

更新日期：1991-08-19 00:00:00

abstract：:Apoptosis is a physiological process for active cell removal. One of its hallmarks is an increased cytosolic Ca2+ content. Several genes involved in apoptosis control have been identified, but their mode of action is not understood in detail. Apoptosis may relate to oncogenesis, in that some malignant tumors may grow ...

journal_title：FEBS letters

authors： Richter C

更新日期：1993-06-28 00:00:00

abstract：:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

journal_title：FEBS letters

authors： Kalipatnapu S,Chattopadhyay A

更新日期：2004-10-22 00:00:00

abstract：:This paper reports the genomic analysis of the strain CBS7064 of Pichia sorbitophila, a homothallic diploid yeast. We sequenced 4829 random sequence tags of about 1 kb and compared them to the Saccharomyces cerevisiae gene products. Approximately 1300 nuclear genes, 22 tRNAs, the rDNA locus, and six mitochondrial gene...

journal_title：FEBS letters

authors： de Montigny J,Spehner C,Souciet J,Tekaia F,Dujon B,Wincker P,Artiguenave F,Potier S

更新日期：2000-12-22 00:00:00

abstract：:50 S ribosomal subunit derivatives without the 5 S RNA-protein complex obtained either by splitting with EDTA or by reconstitution from the 23 S RNA and proteins have been studied by electron microscopy. Removal of the 5 S RNA-protein complex is shown to affect neither the overall morphology of the larger ribosomal su...

journal_title：FEBS letters

authors： Selivanova OM,Gongadze GM,Gudkov AT,Vasiliev VD

更新日期：1986-03-03 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic different...

journal_title：FEBS letters

authors： Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Z

更新日期：2013-09-17 00:00:00

abstract：:Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

journal_title：FEBS letters

authors： Ishida Y,Wyss M,Hemmer W,Wallimann T

更新日期：1991-05-20 00:00:00

abstract：:The mutation of a single leucine residue (L38) to methionine (M) is known experimentally to significantly increase the affinity of the synthetic four-alpha-helix bundle (Aalpha(2))(2) for the anesthetic halothane. We present a molecular dynamics study of the mutant (Aalpha(2)-L38M)(2) peptide, which consists of a dime...

journal_title：FEBS letters

authors： Davies LA,Zhong Q,Klein ML,Scharf D

更新日期：2000-07-28 00:00:00

abstract：:The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...

journal_title：FEBS letters

authors： Sakai W,Sugasawa K

更新日期：2014-10-16 00:00:00

abstract：:To determine the regulatory mechanism for human tyrosine hydroxylase, we examined modulations of the activity of the enzyme from human pheochromocytoma by cyclic AMP-dependent protein kinase, calmodulin-dependent protein kinase II and polyanion. The most remarkable activation was observed when the enzyme was assayed a...

journal_title：FEBS letters

authors： Okuno S,Kanayama Y,Fujisawa H

更新日期：1989-08-14 00:00:00

abstract：:Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

journal_title：FEBS letters

authors： Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

更新日期：1987-02-23 00:00:00